Iranian Journal of Field Crops Research

Iranian Journal of Field Crops Research

Effect of Foliar Application of Glycine and Methanol on Yield and Some Morphological and Physiological Traits of Forage Maize (Zea mays L.) at Three Planting Dates

Document Type : Research Article

Authors
Department of Agronomy and Plant Breeding, Ka. C., Islamic Azad University, Karaj, Iran
Abstract
Introduction
Maize (Zea mays L.) is considered as one of the best forage crops due to its adaptability to various climatic conditions, as well as its production of suitable forage per unit area, high quality of silage, and high absorbability in livestock. Suitable planting date is one of the important factors in determining the yield potential of crops especially in maize, and also the use of methanol and glycine spraying increased plant growth, especially in delayed crops. For this purpose, this study was conducted to investigate the effect of methanol, glycine, and their interaction on three different planting dates on maize in Simorgh city, Mazandaran, Iran. The purpose of this study was to investigate the performance of methanol and glycine on delayed planting dates and whether or not foliar spraying of these compounds can reduce the negative effects of late planting in maize.
Materials and Methods
This study was carried out as a factorial in the form of randomized complete block design with three replications at three separate fields in the promotional research farm of Jihad Agricultural Center, Simorgh city, Mazandaran province, on fodder maize as the second crop after rice harvest during summer and autumn of 2020. The maize hybrid used was Single Cross 201. The treatments were composed of glycine at three levels: [0 (control), 1 and 2 mg l-1], methanol at three levels: [0 (control), 10 and 20% v/v], and three planting dates: [August 6, 16 and 27]. Foliar spraying of maize plants with methanol and glycine solutions was performed in three times during the growing season, including the 4-6 leaf stage, 8-10 leaf stage and before tassel emergence stage, at time of 9-11 am.
Results and Discussion
The results of this study showed that the highest values of dry matter yield of maize fodder was 1944.59 kg ha-1 that related to the interaction treatments of methanol 20 % spraying and glycine 2 ppm spraying in planting date of 6.8.2020. Use of methanol and glycine spraying and also the increasing of their concentration increased the yield and physiological and morphological characteristics of fodder maize. Delay in maize cultivation (27/8/2020) reduced its yield and physiological and morphological characteristics. The highest amount of dry fodder yield 19415.59 kg ha-1 was related to the interaction effects of methanol 20% v/v and glycine 2 mg l-1 on the planting date of August 6 and the lowest dry fodder yield was related to the control treatment 2568.76 kg ha-1 on August 27. The use of methanol and glycine partially reduced the negative effects of the delayed maize planting. Determining the appropriate planting date for crops is of particular importance because it affects the traits and various stages of growth and development and optimizes the efficiency of using environmental factors affecting yield. Delaying maize planting reduces the yield, which is due to a shortening of the vegetative growth period and a decrease in the amount of carbohydrates and minerals transferred to the grain. Methanol increases plant performance by affecting various metabolic pathways such as growth and development and activating genes involved in jasmonic acid biosynthesis and plant defense mechanisms. The use of glycine has increased ear yield due to the production of amino acids during nitrogen metabolism.
Conclusion
Methanol and glycine application reduced the negative effects of delayed planting of forage maize on August 27. The application of methanol 20% v/v and glycine 2 mg L-1 on August 27 was able to increase the dry yield of forage by 168% compared to the control on the same planting date, which can be attributed to the increase in plant growth rate by methanol and glycine. Therefore, to increase the yield of forage corn and reduce losses due to late planting, application of 20% by volume methanol and 2 mg L-1 glycine can be recommended in humid climate areas similar to Mazandaran. However, for a more detailed study, higher concentrations of methanol and glycine can also be recommended.
Keywords
Subjects

Authors retain the copyright. This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0)

  1. Ahmed, N., Zhang, Y., Li, K., Zhou, Y., Zhang, M., & Li, Z. (2019). Exogenous application of glycine betaine improved water use efficiency in winter wheat (Triticum aestivum) via modulating photosynthetic efficiency and antioxidative capacity under conventional and limited irrigation conditions. The Crop Journal, 7(5), 635-650. https://doi.org/10.1016/j.cj.2019.03.004.
  2. Ali, S., Abbas, Z., Seleiman, M. F., Rizwan, M., Yavas, I., Alhammad, B. A., Shami, A., Hasanuzzaman, M., & Kalderis, D. (2020). Glycine betaine accumulation, significance and interests for heavy metal tolerance in plants. Plants, 9(7), 896-911. https://doi.org/10.3390/plants9070896.
  3. Bai, M., Zeng, W., Chen, F., Ji, X., Zhuang, Z., Jin, B., Wang, J., Jia, L., & Peng, Y. (2022). Transcriptase expression profiles reveal response mechanisms to drought and drought-stress mitigation mechanisms by exogenous glycine betaine in maize. Biotechnology Letters Journal, 44, 367-386. https://doi.org/10.1007/s10529-022-03221-6.
  4. Bartlett, M. S. (1937). Properties of sufficiency and statistical tests. Mathematical, Physical and Engineering Sciences, 160, 268-282.
  5. Bortiri, E., & Hake, S. (2007). Flowering and determinacy in maize. Journal of Experimental Botany, 58(5), 909-916. https://doi.org/10.1093/jxb/erm015
  6. Cao, Q., Li, G., Yang, F., Jiang, X., Diallo, L., Zhang, E., & Kong, F. (2019). Maize yield, biomass and grain quality traits responses to delayed sowing date and genotypes in rain-fed condition. Emirates Journal of Food and Agriculture, 31(6), 415-425. https://doi.org/10.9755/ejfa.2019.v31.i6.1969
  7. Dorokhov, Y. L., Sheshukova, E. V., & Komarova, T. V. (2018). Methanol in plant life. Frontiers in Plant Science, 9, 1623-1641. https://doi.org/10.3389/fpls.2018.01623
  8. Downie, A., Miyazaki, S., Bohnert, H., John, P., Coleman, J., Parry, M., & Haslam, R. (2004). Expression profiling of the response of Arabidopsis thaliana to methanol stimulation. Phytochemistry, 65, 2305-2316. https://doi.org/10.1016/j.phytochem.2004.07.006
  9. Gout, E., Aubert, S., Bligny, R., Rebeille, F., Nonomura, A. R., Benson, A. A., & Douce, R. (2000). Metabolism of methanol in plant cells. Carbon-13 nuclear magnetic resonance studies. Plant Physiology, 123(1), 87-96. https://doi.org/10.1104/pp.123.1.287
  10. Gupta, N., & Thind, S. (2017). Grain yield response of drought stressed wheat to foliar application of glycine betaine. Indian Journal of Agricultural Research, 51(3), 287-291. https://doi.org/10.18805/ijare.v51i03.7920
  11. Hamani, A. K. M., Chen, J., Soothar, M. K., Wang, G., Shen, X., Gao, Y., & Qiu, R. (2021). Application of exogenous protectants mitigates salt-induced Na+ toxicity and sustains cotton (Gossypium hirsutum) seedling growth: Comparison of glycine betaine and salicylic acid. Plants, 10(2), 380-395. https://doi.org/10.3390/plants10020380
  12. Hanson, A. D., & Roje, S. (2001). One-carbon metabolism in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology, 52, 119-37. https://doi.org/10.1146/annurev.arplant.52.1.119
  13. Haghighi, P., Habibi, D., Mozafari, H., Thani, B., & Sadeghi Shua, M. (2020). Study of the effect of methanol and amino acid glycine spraying on yield and some physiological traits in different fodder beet cultivars. Sugar Beet Journal, 36(2), 185-201. (in Persian)
  14. Hussain, N., Hussain, A., Anwar Bhat, M., Aliwani, O., Ahmad, T., Mir, A. H., Jelani, F., Kauser, S., Sheikh, T., & Fatima, N. (2022). Effect of establishment methods, planting dates and hybrids on phenology and yield of sweet corn (Zea mays saccharata L) under temperate conditions. The Pharma Innovation Journal, 11(4), 626-629.
  15. Kalantar Ahmadi, S. A., & Daneshian, J. (2021). The effect of foliar spraying with ascorbic acid, salicylic acid and methanol on the physiological characteristics and the reduction of late planting damage of rapeseed. Journal of Crops Improvement, 23(2), 392-377. (in Persian)
  16. Khalily, M., Moghaddam, M., Kanouni, H., & Asheri, E. (2010). Dissection of drought stress as a grain production constraint of maize in Iran. Asian Journal of Crop Science, 2(2), 60-69. https://doi.org/10.3923/ajcs.2010.60.69
  17. Khamer, M., Mobasr, H. R., & Kikhani, S. (2018). Investigating the effect of time and different levels of methanol spraying on the yield and yield components of mung bean plant (Vigna radiate). Agricultural Research in the Desert Margin, 15(2), 83-91.
  18. Korkmaz, A., Şirikçi, R., Kocaçınar, F., Değer, O., & Rıza Demirkırıan, (2012). Alleviation of salt-induced adverse effects in pepper seedlings by seed application of glycinebetaine. Scientia Horticulturae, 148, 197-205. https://doi.org/10.1016/j.scienta.2012.09.029.
  19. Liaqat, W., Akmal, M., & Ali, J. (2018). Sowing date effect on production of high yielding maize varieties Sarhad. Journal of Agriculture, 34(1), 102-113.
  20. Lixin, Z., ShengXiu, L., & ZongSuo, L. (2009). Differential plant growth and osmotic effects of two maize (Zea mays) cultivars to exogenous glycine betaine application under drought stress. Plant Growth Regular, 58, 297-305. https://doi.org/10.1007/s10725-009-9379-7
  21. Long, N. V., Assefa, Y., Schwalbert, R., & Ciampitti, I. A. (2017). Maize yield and planting date relationship: A synthesis-analysis for US high-yielding contest-winner and field research data. Frontiers in Plant Science, 8, 16-21. https://doi.org/10.3389/fpls.2017.02106
  22. Mahmoud Soltani, S., Hosseini Chaleshtori, M., Tajaddodi Talab Rashti, K., Shukri Vahad, H., & Shakouri Katigeri, M. (2022). Effect of glycine amino acid chelated zinc and iron on yield, and macro and micronutrient contents of rice aerial tissues. Iranian Journal of Soil and Water Research, 53(4), 763-776. (in Persian). https://doi.org/10.22059/ijswr.2022.337158.669181
  23. Nazeri, P., Shirani Rad, A. H., Valad Abadi, S. A. Mirakhori, M., & Hadidi Masoule, E. (2018). Effect of sowing dates and late season water deficit stress on quantitative and qualitative traits of canola cultivars. Outlook Agriculture, 47(4), 291-297. https://doi.org/10.1177/0030727018793658
  24. Paknejad, F., Mirakhori, M., Al-Ahmadi, M. J., Tookalo, M. R., Pazoki, A. R., & Nazeri, P. (2009). Physiological response of soybean (Glycine max) to foliar application of methanol under different soil moistures. American Journal of Agricultural & Biological Science, 4, 311-318. https://doi.org/10.3844/ajabssp.2009.311.318
  25. Plett, S. (1992). Comparison of seasonal thermal indices for measurement for measurement of corn maturity in a Prairie environment. Canadian Journal of Plant Science, 72, 1157-1162. https://doi.org/10.4141/cjps92-141
  26. Shemi, R., Wang, R., Gheith, M. S., Athar Hussain, H., Hussain, S., Irfan, M., Cholidah, L., Zhang, K., Zhang, S., & Wang. L. (2021). Effects of salicylic acid, zinc and glycine betaine on morpho-physiological growth and yield of maize under drought stress. Scientific Reports, 28(3), 1-12. https://doi.org/10.1038/s41598-021-82264-7
  27. Zhou, B., Yue, Y., Sun, X., Ding, Z., Ma, W., & Zhao, M. (2017). Maize kernel weight responses to sowing date-associated variation in weather conditions. The Crop Journal, 5(1), 43-51. https://doi.org/10.1016/j.cj.2016.07.002
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  • Receive Date 03 April 2025
  • Revise Date 16 May 2025
  • Accept Date 01 June 2025
  • First Publish Date 29 June 2025