Effect of Salinity and Drought Stresses on Germination Stage and Growth of Black Cumin (Bunium Persicum Boiss)

Document Type : Research Article

Authors

• H. R. Saeedi Goraghani ¹
• A Ranjbar Fordoei ²
• M Soleimani Sardo ³
• M. J Mahdavi ⁴

¹ University of Tehran

² University of Kashan

³ University of Jiroft

⁴ Payame Noor University

10.22067/gsc.v15i1.20002

Abstract

Introduction
Range plants have important and crucial roles in medicinal industry andtogether with scarcity and low quality of the water and soil resources, prevent a quick recovery of the soil plant covering. Because of these restrictions, it is important to consider the use of salt and drought tolerant species for plantation and to preserve plant cover. In this sense, the use of native species such as black cumin (Bunium persicum Boiss) may be of interest due to their medicinal characteristics and potential ability to adapt to adverse conditions (dry and saline conditions). Black cumin (B. persicum) as a medicinal plant plays a vital role in Iranian medicine so there is a need to know about the factors affecting their growth and propagation.

Materials and Methods
To investigate the effects of drought and salt stresses on germination and growth in black cumin two separate experiments were conducted. Drought stress was applied through incubation in four different concentrations of PEG 6000 that provide solutions with water potentials ranging from -0.2 to -0.8 MPa (including control and four levels of dryness). Salinity treatments (including control and four levels of salinity) were prepared by adding molar concentrations of NaCl to provide a range of salinity from 50 to 300 mM. Germination percentage and speed was calculated by computation of germinated seeds every day. Growth parameters (rootlet, shoot and seedling length total), allometric index and seed vigority were obtained accordingly.

Results and Discussion
Seeds under both drought and salt stress showed significant reduction in germination percentage, germination rate, radicle length, plumule length, and alometric and seed vigor indices. This trend was much pronounced under high levels of NaCl and low levels of water potentials, so that germination at Ψs = -0.6 MP was completely stopped.

Conclusions
Assessment of drought and salt stresses on germination and growth in black cumin is very important in the management of its planting. With increasing salinity and drought levels, the germination reduced significantly. Although due to higher germination of seeds in different levels of salinity rather than drought, this species seems to be more sensitive to drought stress.

Keywords

• Bunium persicum
• Drought stress
• Germination
• Salt stress

20.1001.1.20081472.1396.15.1.1.5

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