Study the Effect of Cadmium Stress on Morphological Traits and Cadmium Accumulation in Barley

Document Type : Research Article

Authors

• M Sadlou ¹
• A Asghari ¹
• A Akhgar ²

¹ University of Mohaghegh Ardabili

² Valieasr University of Rafsangan

10.22067/gsc.v12i4.24913

Abstract

Cadmium is a toxic metal that affect human and other organism’s life. In other to study the effect of Cd stress on morphological traits in 40 barley genotypes, a split plot experiment was conducted based on completely randomized design with three replications at the University of Mohaghegh Ardabili in 2011. CdCl2 was used in two levels (control and 50 μmol). Seeds were cultivated in perlite and Pit moss medium, and Hogland nutrient solutions (with and without Cd) were used for irrigation. After maturity stage morphological traits were measured. Analysis of variance showed considerable variation for Cd accumulation between the genotypes. At control level, the genotypes had not accumulation of Cd. At Cd stress level, the grains of Abidar, Dasht, Sahra, GhareArpa, Yensivi, Daytonrani and Sahand varietis and some of lines had not accumulation of Cd and the line number of 18 (F-A2-11) and the Bahman varietis had highest Cd accumulation. Also, the highest accumulation of Cd in the shoot and leaves was observed in the line number of 18. Then, the lines 24, 25, 29 and Bahman variety had higher Cd accumulation in the shoot and leaves. The lines 3, 6, 7, 12, 13, 16 and 20 and Yensivi and Makoei varieties had the lowest Cd accumulation in the shoot and leaves. In total, Cd stress reduced measured traits mean in most genotypes. Cluster analysis of the genotypes based on measured traits categorized the genotypes into five groups. At control and Cd stress levels. The Gharearpa variety had higher mean for most traits and at control and stress levels located in groups that had higher traits average than other groups.

Keywords

• barley
• Genetic diversity
• Stress
• Tolerance of Cultivars
• Toxic metals

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References

1- ثواقبی، غ. ر. و م. ج. ملکوتی. 1379. اثرات روی و کادمیم بر غلظت عناصر و ترکیب شیمیایی دانه گندم. مجله آب و خاک، ویژه نامه کشاورزی پایدار، مؤسسه تحقیقات آب و خاک 9(12): 54-65.

2- Arduini, I., A. Masoni, M. Mariotti, and L. Ercoli. 2004. Low cadmium application increase miscanthus growth and cadmium translocation. Environmental and Experimental Botany, 52: 89-100.

3- Beyersmann D. 2002. Effect of carcinogenic metal on gene expression. Toxical Letter, 127: 63-68.

4- Ci D., D. Jiang, B. Wollenweber, T. Dai, Q. Jing and W. Cao. 2010. Genetic variance in cadmium tolerance and accumulation in wheat materials differing in ploidy and genome at seedling stage. Journal of Agronomy andCrop Science, 196: 302-310.

5- Dudjak J., J. Lachman, D. Miholova, D. Kolihova and V. Pivec. 2004. Effect of cadmium on polyphenol content in young barley plants (Hordeum vulgare L.). Plant Soil and Environment, 11: 471-477.

6- Eriksson J., I. Oborn, G. Jansson and A. Andersson. 1996. Factors influencing Cd-content in crops. Results from Swedish field Investigations. Swedish Journal of Agricultural Research, 26: 125–133.

7- Esfandiari E., M. Shokrpour and S. Alavi-Kia. 2010. Effect of Mg deficiency on antioxidant enzymes activities and lipid peroxidation. Journal of Agricultural Science, 3: 131-136.

8- Graham R.D., R.M. Welch, D.A.Saunders, I. Ortiz-Monasterio, H.E. Bouis and M. Bonierbale. 2007. Nutritious subsistence food systems. Advanced Agriculture, 92: 69–74.

9- Greger M. and M. Lofstedt. 2004. Comparison of Uptake and Distribution of Cadmium in Different Cultivars of Bread and Durum Wheat. Crop Science, 44: 501–507.

10- Hall J.L. 2002. Cellular mechanism for heavy metal detoxification and tolerance. Journal of experimental botany, 53: 1-11.

11- Golldack D. and V.J.K. Dietz. 2003. Expression of subtilisin-like serin proteases in Arabidopsis thaliana is cell-specific and responds to jasmonic acid and heavy metals with developmental differences. Physiologia Plantarum, 118: 64-73.

12- Hassan M.J., G. Zhang, F. Wu, K. Wei and Z. Chen. 2005. Zinc alleviates growth inhibition and oxidative stress caused by cadmium in rice. Journal of Plant Nutrition and Soil Science, 168: 255-261.

13- Hassan M.J., Z. Zhu, B. Ahmad and Q. Mahmood. 2006. Influence of cadmium toxicity on rice genotypes as affected by zinc, sulfur and nitrogen fertilizers. Caspian Journal of Environmental Science, 4(1): 1-8.

14- Howden R., C.R. Andersen, P.B. Goldsbrough and C.S. Cobbet. 1995. Cadmium-sensitive, glutathione mutant of Arabidopsis thaliana. Plant Physiology, 107: 1067-1073.

15- Jianguo L., Q. Min, C. Guoliang, Y. Jianchang and Z. Qingsen. 2006. Uptake and translocation of Cd in different rice cultivars and the relation with Cd accumulation in rice grain. Journal of Hazardous Materials, 143: 443–447.

16- Kennedy C.D. and F.A.N. Gonsalves. 1987. The action of divalent zinc, cadmium, mercury, copper and lead on the transroot potential and H+ efflux of excised roots. Journal of Experimental Botany, 38: 800-817.

17- Khan N.A., S. Singh and R. Nazar. 2007. Activities of antioxidative enzymes, sulphur assimilation, photosynthetic activity and growth of wheat cultivars differing in yield potential under cadmium stress. Journal of Agriculture and Crop Science, 193: 435-444.

18- Kinraide T.B., P.R. Ryan and L.V. Kochian. 1992. Interactive effects of Al3+, H+ and other actions on root elongation considered in terms of cell-surface electrical potential. Plant Physiology, 99: 1461-1468.

19- Lee S.H., S.S. Jew, P.S. Chang, I.J. Hong, E.S. Hwang, K.S. Kim and K.T. Kim. 2003. Free radical scavenging effect and antioxidant activities of barley leaf blades. Food Science and Biotechnology, 12: 268–273.

20- Mane A.V., R. Sankpal and S. Ambawade. 2010. Cadmium chloride induced alteration in growth and cadmium accumulation in Triticum aestivum. International Journal of Pharmaceutical Chemistry, 5: 206-215.

21- Prince W.S., P. Senthil Kumar, K.D. Doberschutz and V. Subburam. 2002. Cadmium toxicity in mulberry plants with special reference to the nutritional quality of leaves. Journal of Plant Nutrient, 25: 689 –700.

22- Perriguey J., T. Sterckeman and J.L. Morel. 2007. Effect of rhizosphere and plant related factors on the cadmium uptake by maize (Zea mays L.). Environmental and Experimental Botany, 10: 201-215.

23- Qian H., J. Li, L. Sun, W. Chen, G.D. Sheng, W. Liu and Z. Fu. 2009. Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription. Aquatic Toxicology, 94: 56–61.

24- Romero-Puertas M.C., M. Rodriguez-Serrano, F.J. Corpas, M. Gomez, L.A. delRio and L.M. Sandalio. 2004. Cadmium-induced subcellular accumulation of O2- and H2O2 in pea leaves. Plant Cell and Environment, 27: 1122-1134.

25- Sandalio L.M., H.C. Dalurzo, M. Gomes, M. Romero-Puertas and L.A. delRio. 2001. Cadmium-induced changes in the growth and oxidative metabolism of pea plants. Journal of Experimental Botany, 52: 2115–2126.

26- Shanker A.K., C. Cervantes, H. Loza-Tavera and S. Avudainayagam. 2005. Cadmium toxicity in plants. Environment International, 31: 63-68.

27- Shi G.R. and Q.S. Cai. 2008. Photosynthetic and anatomic responses of peanut leaves to cadmium stress. Photosynthetica, 46(4): 627–630.

28- Tamas L., J. Huttova and I. Mistrik. 2003. Inhibition of Al-induced root elongation and enhancement of Al induced peroxidase in Al-sensitive and Al-resistant barley cultivars are positively correlated. Plant Soil, 250: 193–200.

29- Vassilev A. and I. Yordanov. 1997. Reductive analysis of factors limiting growth of cadmium–treated plants: A review. Plant Physiology, 23(3-4): 114-133.

30- Vassilev A., I. Yordanov and T. Tsonev. 1998. Physiological responses of two barley cultivars to soil pollution with cadmium. Environmental Pollution, 100: 1–7.

31- Vassilev A. and P. Manolov. 1999. Chlorophyll fluorescence of barley (Hordeum vulgare L.) seedlings grown in excess of Cd. Bulg. Journal of Plant Physiology, 25: 67-76.

32- Vassilev A. 2003. Barley seedlings as bio-indicators for water contamination by cadmium. Journal of Environmental Protection and Ecology, 4(2): 354-360.

33- Vassilev A. 2003. Physiological and agro ecological aspects of cadmium interactions with barley plants: An overview. Journal of Central Europian Agriculture, 4(1): 65-76.

34- Vecera Z. 1999. Additional comments about trace elements in crop plants. Academy of Science, Czech Republic, Brno,veveri, 97: 61-142.

35- Wang H., S.C. Zhao, R.L. Liu, W. Zhou and J.Y. Jin. 2009. Changes of photosynthetic activities of maize (Zea mays L.) seedlings in response to cadmium stress. Photosynthetica, 47(2): 277-283.

36- Woodies T.C., G.B. Hunter and F.J. Johnson. 1977. Statistical studies of matrix effects on the determination of cadmium and lead in fertilizer and material and plant tissue by flame atomic absorption spectrophotometry. Analytica Chemica Acta, 90:127-136.

37- Wong S.C., X.D. Li, G. Zhang, S. Qi and Y.S. Min. 2002. Heavy metals in agriculture soils of the Pear River Delta, South China. Environmental Pollution, 119: 33-44.

38- Wu F.B. and G.P. Zhang. 2002. Alleviation of cadmium-toxicity by application of zinc and ascorbic acid in barley. Journal of Plant Nutrients, 25: 2745-2761.

39- Wu F.B., G.P. Zhang and P. Dominy. 2003. Four barley genotypes respond differently to cadmium. Lipid peroxidation and activities of antioxidant capacity. Environmental and Experimental Botany, 50: 67–78.