اثر همزیستی قارچ مایکوریزا و کاربرد برگی اسیدهای آمینه بر برخی صفات رشدی و روغن گیاه دارویی همیشه بهار (Calendula officinallis L.)

نوع مقاله : مقاله پژوهشی

نویسنده

گروه کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

به‌منظور بررسی اثر محلول‌پاشی اسیدهای آمینه و همزیستی قارچ مایکوریزا بر صفات کمی و کیفی گیاه دارویی همیشه بهار (Calendula officinalis L.) آزمایشی به‌صورت فاکتوریل در قالب طرح پایه بلوک کامل تصادفی با سه تکرار، طی سال‌های زراعی 1399-1398 و 1398- 1397 در مزرعه تحقیقاتی دانشگاه آزاد اسلامی واحد تاکستان اجرا شد. عوامل آزمایشی شامل سه گونه قارچ مایکوریزا (شاهد، Glomus mosseae، G.etunicatum و G. intraradices) و سطوح اسید آمینه سورن (این ترکیب تجاری حاوی انواع اسید آمینه‌های ضروری برای گیاه است) شامل بدون محلول‌پاشی و محلول‌پاشی در دو مرحله ساقه‌دهی و گل‌دهی بود. نتایج نشان داد که کاربرد اسید آمینه و قارچ مایکوریزا گونه G. mosseae نسبت به تیمار عدم استفاده از قارچ مایکوریزا و کاربرد اسید آمینه افزایش بسیار معنی‌داری در درصد کلونیزاسیون ریشه داشت. کاربرد G. mosseae  در همیشه بهار باعث افزایش 33% شاخص کلروفیل شد. همچنین نتایج نشان دادند درصد و عملکرد روغن در اثر کاربرد برگی اسید آمینه و استفاده از G. mosseae به‌ترتیب به میزان 25% و 70%  نسبت به تیمار عدم استفاده از اسید آمینه و مایکوریزا افزایش یافت. بیشترین عملکرد اسانس نیز از تیمار G. mosseae با مقدار 74/7 کیلوگرم در هکتار به‌دست آمد. به‌طور کلی، نتایج نشان داد که تلقیح گیاه دارویی همیشه بهار با قارچ مایکوریزا به‌ویژه گونه mosseae می‌تواند در جذب عناصر غذایی در جهت رشد بهتر این گیاه استفاده شود و کاربرد برگی اسید آمینه با در اختیار قرار دادن نیتروژن برای گیاه، روند رشد و توسعه گیاه را بهبود بخشد.

کلیدواژه‌ها

موضوعات


  1. Amouii, A. M., Mojahed, M., and Mojahed, M. 2017. Pot marigold Production Entrepreneurship Package. Publishing Asrar ghalam. 93 pages.
  2. Aqhawani Shajari, M., Rezvani Moghadam, P., Ghorbani, R., and Nasiri Mahallati, M. 2015. Effects of application of organic, biological and chemical fertilizers on quantitative and qualitative yield of (Coriandrum sativum ). Horticultural Sciences 29 (4): 500-486.
  3. Arjmand Alavi, M., Hatamzadeh, A., and Ehteshami, S. M. 2014. Effect of bulb inoculation with four species mycorrhizal fungi on quantitative and qualitative yield of two lily species. Iranian Journal of Seed Science and Research 1 (2): 57-65.
  4. Auge, R. M. 2001. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11(1): 3-42. https://doi.org/10.1007/s005720100097.
  5. Chaudhary, V., Kapoor, R., and Bhatnagar, A. K. 2007. Effect of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua Mycorrhiza 17: 581-587. https://doi.org/10.1007/s00572-007-0135-4.
  6. Diagne, N., Ngom, M., Ibrahima Djighaly, P., Fall, D., Hocher, V., and Svistoonof, S. 2020. Roles of Arbuscular Mycorrhizal Fungi on Plant Growth and Performance: Importance in Biotic and Abiotic Stressed Regulation. Diversity 12: 370. DOI:10.3390/d12100370.
  7. Faten, S., Abd El-Aal, F. S., Shaheen, A. M., Ahmed, A. A., and Mahmoud, A. R. 2010. Effect of foliar application of urea and amino acids mixtures as antioxidants on growth, yield and characteristics of squash. Agriculture and Biological Sciences 6: 583-588.
  8. Fathi, Sh., Azarpira, E., Sharafi, Y., and Najafian, S. 2020. Effect of some amino acids based biostimulants on Medicinal Mint (Mentha spicat) under salinity stress. Scientific Journal of Horticultural Nutrition 2 (2): 154-173.
  9. Ghazi Manas, M., Banj Shafiee, S., Haj Seyed Hadi, M. R., and Darzi, M. T. 2013. Effects of vermicompost and nitrogen on quantitative and qualitative yeild of chamomile (Matricaria chamomilla). Iranian Journal of Medicinal and Aromatic Plants 29 (2): 269-280. (in Persian).
  10. Gogoi, P., and Singh, R. K. 2011. Different effect of some arbuscular mycorrhizal fungi on growth of Piper longum (Piperaceae). Indian Journal of Sciences and Technology 4(2): 119-125.
  11. Golubkina, N., Logvinenko, L., Novitsky, M., Zamana, S., Sokolov, S., Molchanova, A., Shevchuk, O., Sekara, A., Tallarita, A., and Caruso, G. 2020. Yield, essential oil and quality performances of Artemisia dracunculus, Hyssopus officinalis and Lavandula angustifolia as affected by arbuscular mycorrhizal fungi under organic management. Plants 9: 375. DOI: 10.3390/plants9030375. https://doi.org/10.3390/plants9030375.
  12. Golzadeh, H., Mehrafarin, A., Naghdi Badi, H., Fazeli, F., Ghaderi, A., and Zarincheh, N. 2011. Effects of biostimulants on quantitative and qualitative yield of German chamomile. Journal of Medicinal Plants 11(41): 195-207. (in Persian).
  13. Gunes, A. K., Zengin, G., Ibrahime Sinan, K., Fawzi Mahomoodally, M., Picot-Allain, M. C. N., Cakýr, O., Bensari, S., Abdullah Yýlmaz, M., Gallo, M., and Montesano, D. 2020. A Comparative Bio-Evaluation and Chemical Profiles of Calendula officinalis L. Extracts Prepared via Different Extraction Techniques. Applied Sciences 10: 5920. DOI: 10.3390/app10175920.
  14. Haghir Ebrahimabadi, A., Hatami, M., Karimzadeh Asl, K., and Ghorbanpour, M. 2017. Effect of Mycorrhizal Fungi and Biophosphor Fertilizer on Growth Features, Yield and Yield Components, and Essntial Oil Constituents in Cuminum cyminum Journal of Medicinal Plants 17 (2): 174-184. (in Persian).
  15. Hamzei, J., and Salimi, F. 2014. Root Colonization, Yield and Yield Components of milk thistle (Silybum marianum) Affected by Mycorhizal Fungi and Phosphorus Fertilizer. Journal of Agricultural Knowledge and Sustainable Production 24 (4): 85-96. (in Persian).
  16. Hazzoumi, Z., Moustakime, Y., Elharchli, E., and Amrani Joutei, Kh. 2015. Effect of arbuscular mycorrhizal fungi and water stress on ultrastructural change of glandular hairs and ssential oil compositions in Ocimum gratissimum. Chemical and Biological Technologies in Agriculture 2: 1-10. https://doi.org/10.1186/s40538-017-0102-z.
  17. Khaosaad, T., Vierheilig, H., Nell, M., ZitterlEglseer, K., and Novak, J. 2006. Arbuscular mycorrhiza alters the concentration of essential oils in oregano (Origanum sp., Lamiaceae). Mycorrhiza 16 (6): 443-446. https://doi.org/10.1007/s00572-006-0062-9.
  18. Kheiri, Z., Moghadam, M., and Moradi, M. 2020. Investigation of the effect of different species of mycorrhizal fungi on some growth indices, photosynthetic pigments, flavonoid and carotenoid content of pot marigold flower. Scientific Journal of Garden Nutrition 3 (1): 37-50 . (in Persian).
  19. Khoramdel, S., Koocheki, A., Nassiri Mahalati, M., and Ghorbani, R. 2008. Application effects of biofertilizers on the growth indices of black cumin (Nigella sativa). Iranian Journal of Crop Research 6 (2): 285-294. DOI: 10.22067/GSC.V6I2.2435. (in Persian).
  20. Kormanik, P. P., and McGraw, A. C. 1982. Quantification of vesicular-arbuscular mycorrhizae in plant roots. In Methods and Principles of Mycorrhizal Research (N. C. Schenck, Ed.). pp. 37-45. American Phytopathological Society, st Paul.
  21. Krishnasree, R. K., Raj, S. K., and Chacko, S. R. 2021. Foliar nutrition in vegetables: A review. Journal of Pharmacognosy and Phytochemistry 10(1): 2393-2398. DOI: https://doi.org/10.22271/phyto.2021.v10.i1ah.13716.
  22. Maleki, S., Aghayari, F., Ardakani, M. R., and Rajali, F. 2014. Investigation of the possibility of improving root growth of two lentil cultivars (Lens culinaris) using mycorrhizal and azospirillum symbiosis under dryland conditions. Journal of Agricultural Ecology 6 (4): 779-787. (in Persian).
  23. Moghadasan, Sh., Safipour Afshar, A., and Saeid Nematpour, F. 2016. The Role of Mycorrhiza in Drought Tolerance of pot Marigold (Calendula officinalis). Journal of Crop Ecophysiology 9 (4): 521-532. (in Persian).
  24. Mohammadi, S., Tabrizi, L., Delshad, M., and Moteshare Zadeh, B. 2013. Investigation of growth and yield of pot marigold (Calendula officinalis) under arbuscular mycorrhizal fungi symbiosis and heavy metal stress conditions. Iranian Journal of Agroecology 3 (2): 48-59. (in Persian).
  25. Moradi Marjaneh, E., Golavi, M., Ramroudi, M., and Salouki, M. 2018. Investigation of some qualitative characteristics of rosemary plant under the influence of foliar application of various nutrients at different harvest times. Journal of Crop Production 11 (4): 119-134. DOI: 10.22069/ejcp.2019.13979.2064.
  26. Nasholm, T., Kielland, K., and Ganeteg, U. 2009. Uptake of organic nitrogen by plants. New Phytology 182: 31-48. https://doi.org/10.1111/j.1469-8137.2008.02751.x.
  27. Omidbeigi, R. 2000. Production and processing of medicinal plants. Volume I, Second Edition, Tarahan Publication. 424 pp. (in Persian).
  28. Pouryousef Miyandoaf, M., and Shahrvan, N. 2014. The effect of foliar application of amino acids at different times on yield and yield components of maize. Journal of Crop Physiology - Islamic Azad University, Ahvaz Branch 23: 21-32. (in Persian).
  29. Raeisi, M., Farahani, L., and Palashi, M. 2014. Changes of qualitative and quantitative properties of radish (Raphanus sativus) under foliar spraying through amino acid. International Journal of Biosciences 4 (1): 463-468. DOI: http://dx.doi.org/10.12692/ijb/4.1.463-468.
  30. Rahimi, A., Jahanbin, Sh., salehi, A., and Faraji, H. 2016. Effect of mycorrhiza on morphological characteristics, phenolic compounds and chlorophyll fluorescence of (Borago officinails) under drought stress. Journal of Plant Environmental Physiology 11 (42): 46-55. (in Persian).
  31. Rahimi, A., Jalalian, Sh., Salehi, A., and Farajee, H. 2017. The effect of mycorrhiza on yield, oil content and water use efficiency of medicinal plant of Borage (Borago officinails) under water stress. Iranian Journal of Horticultural Science 49 (2): 407-415. DOI: 10.22059/ijhs.2017.220274.1127.
  32. Rahimi, N., Jalalian, J., Pirzad, A., and Gholinezhad, E. 2019. Evaluation of grain and oil yield changes of linseed (Linum usitatissimum) as affected by bio-fertilizers and supplementary irrigation. Scientific Journal of Plant Ecophysiology 12 (41): 41-52. (in Persian).
  33. Rahmani, N., Valadabadi, S. A., Daneshian, J., and Bigdeli, M. 2008. The effect of different levels of drought stress and nitrogen on oil yield in pot marigold. Iranian Journal of Medicinal and Aromatic Plants Research 24 (1): 101-108. (in Persian).
  34. Rekha, B., Shruti, C., Rashmi, S., Sharma, A. K., and Johri, B. 2009. Effect of arbuscular mycorrhizal fungi on the growth and nutrient status of Dalbergia sissoo. Tropical Ecology Journal 50: 231-242.
  35. Rezakhani, A., and Haj Seyed Hadi, M. R. 2017. Effect of manure and foliar application of amino acids on growth characteristics, seed yield and essential oil of coriander (Coriandrum sativum). Iranian Journal of Field Crop Science 48 (3): 777-786. DOI: 10.22059/ijfcs.2017.210745.654144. (in Persian).
  36. Rafiee, H., Mehrafarin, A., Qaderi, A., Kalate Jari, S., and Naghdi Badi, H. 2013. Phytochemical, Agronomical and Morphological Responses of Pot Marigold (Calendula officinalis) to Foliar Application of Bio-stimulators (Bioactive Amino Acid Compounds). Journal of Medicinal Plants 12 (47): 48-61.
  37. Saburi, M., Haj Seyed Hadi, M. R., and Darzi, M. T. 2014. The effect of foliar application of amino acids and application of nitrogen-fixing bacteria on the growth characteristics of green basil. The First International Congress and the Thirteenth National Congress of Agronomy and Plant Breeding. Iran
  38. Safari Sinegani, A. A., and Elyasi Yeganeh, M. 2017. The occurrence of arbuscular mycorrhizal fungi in soil and root of medicinal plants in Bu-Ali Sina garden in Hamadan, Iran. Biological Journal of Microorganism 5 (20): 43-59. DOI: 22108/bjm.2017.21145. (in Persian).
  39. Slavov, A., Ognyanov, M., and Vasileva, I. 2020. Pectic polysaccharides extracted from pot marigold (Calendula officinalis) industrial waste. Food Hydrocolloids 101: 105545. DOI: 10.1016/j.foodhyd.2019.105545.
  40. Soleimani, A. 2015. Effect of plant density on light absorption in canopy and growth indices of sunflower cultivars. Journal of Crop Physiology 28: 107-123. (in Persian).
  41. Souri, M. K., and Yarahmadi, B. 2015. Effect of amino chelates foliar application on growth and development of Marigold (Calendula officinalis) plant. Crop Production Technology 15 (2): 109-119. (in Persian).
  42. Torabahmadi, S., Abedy, B., and Saberali, S. F. 2019. Evaluation of some quantitative and qualitative characteristics of Pistachio plant in Response to amino acid compounds and seaweed extract. Journal of Agricultural Knowledge and Sustainable Production 29 (4): 189-204. (in Persian).
  43. Wahba, H. E., Motawe, H. M., and Ibrahim, A. Y. 2015. Growth and chemical compositionof Urtica pilulifera plant as influenced by foliar application of some aminoacids. Journal of Materials and Environmental Science 6 (2): 499-509.
  44. Zhang, X. F., Hu, Z. H., Yan, T. X., Lu, R. R., Peng, C. L., Li, S. S., and Jing, Y. X. 2019. Arbuscular mycorrhizal fungi alleviate Cd phytotoxicity by altering Cd subcellular distribution and chemical forms in Zea mays. Ecotoxicol Environ Saf 171: 352-360. DOI: 10.1016/j.ecoenv.2018.12.097.
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