بهمنظور بررسی اثر محلولپاشی اسیدهای آمینه و همزیستی قارچ مایکوریزا بر صفات کمی و کیفی گیاه دارویی همیشه بهار (Calendula officinalis L.) آزمایشی بهصورت فاکتوریل در قالب طرح پایه بلوک کامل تصادفی با سه تکرار، طی سالهای زراعی 1399-1398 و 1398- 1397 در مزرعه تحقیقاتی دانشگاه آزاد اسلامی واحد تاکستان اجرا شد. عوامل آزمایشی شامل سه گونه قارچ مایکوریزا (شاهد، Glomus mosseae، G.etunicatum و G. intraradices) و سطوح اسید آمینه سورن (این ترکیب تجاری حاوی انواع اسید آمینههای ضروری برای گیاه است) شامل بدون محلولپاشی و محلولپاشی در دو مرحله ساقهدهی و گلدهی بود. نتایج نشان داد که کاربرد اسید آمینه و قارچ مایکوریزا گونه G. mosseaeنسبت به تیمار عدم استفاده از قارچ مایکوریزا و کاربرد اسید آمینه افزایش بسیار معنیداری در درصد کلونیزاسیون ریشه داشت. کاربرد G. mosseae در همیشه بهار باعث افزایش 33% شاخص کلروفیل شد. همچنین نتایج نشان دادند درصد و عملکرد روغن در اثر کاربرد برگی اسید آمینه و استفاده از G. mosseaeبهترتیب به میزان 25% و 70% نسبت به تیمار عدم استفاده از اسید آمینه و مایکوریزا افزایش یافت. بیشترین عملکرد اسانس نیز از تیمار G. mosseae با مقدار 74/7 کیلوگرم در هکتار بهدست آمد. بهطور کلی، نتایج نشان داد که تلقیح گیاه دارویی همیشه بهار با قارچ مایکوریزا بهویژه گونه mosseae میتواند در جذب عناصر غذایی در جهت رشد بهتر این گیاه استفاده شود و کاربرد برگی اسید آمینه با در اختیار قرار دادن نیتروژن برای گیاه، روند رشد و توسعه گیاه را بهبود بخشد.
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