In Vitro Optimization of Rooting in Two Genotype of Lentil (Lens culinaris Medik.)

Ghasemi Omran, V; Bagheri, A

doi:10.22067/gsc.v8i1.7397

In Vitro Optimization of Rooting in Two Genotype of Lentil (Lens culinaris Medik.)

Document Type : Research Article

Authors

V Ghasemi Omran

A Bagheri

Ferdowsi University of Mashhad

10.22067/gsc.v8i1.7397

Abstract

Abstract
Establishing of an efficient and repeatable regeneration protocol is one of the basic prerequirements for gene transformation and plant breeding. Lentil, because of rooting problems, is a recalcitrant legume in respect of whole regeneration. For this, we examined two methods in vitro and in vivo flowing in vitro method to induce root on regenerated shoots. In vitro-in vivo method was better than in vitro for rooting of regenerated shoots. The shoots regenerated from medium containing 2 mg/lit BAP produced higher frequency of rooting (70%) than medium containing 3, 4 mg/lit BAP that represents the inhibitory effect of cytokenins on root induction. IBA (10 mg /lit) as a overnight pretreatment, induced higher frequency of rooting than 3 days pretreatment while, NAA hormone with similar concentration as a 3 days pretreatment produced highest frequency of rooting (80%). Genotypes had no effect on percent, number and length of produced roots.

Keywords: Lentil, Rooting, Auxin, explant, in vitro