تأثیر کاربرد اسید سالیسیلیک و سدیم نیتروپروساید بر ویژگی‌‌های فیزیولوژیک گیاه حنا (.Lowsonia inermis L) تحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران

چکیده

جهت ارزیابی تأثیرپذیری برخی از صفات فیزیولوژیک و عملکرد گیاه دارویی حنا از محلول‌پاشی با اسید سالیسیلیک و سدیم نیتروپروساید تحت شرایط تنش آزمایشی به‌صورت کرت‌های خرد‌شده بر پایه طرح بلوک‌های کامل تصادفی با چهار تکرار در شهرستان قلعه گنج استان کرمان در سال زراعی 1400 اجرا گردید. در این آزمایش، عامل اصلی شامل سه سطح آبیاری (50 درصد، 75 درصد و 95 درصد از ظرفیت زراعی) و عامل فرعی شامل محلول‌پاشی تنظیم‌کننده رشد (شاهد، اسید سالیسیلیک، سدیم نیتروپروساید، و ترکیب اسید سالیسیلیک و سدیم نیتروپروساید) بود. در این آزمایش، صفاتی از قبیل ارتفاع بوته، شاخص سطح برگ، محتوای پرولین، محتوای پروتئین، غلظت مالون دی‌آلدئید، فعالیت آنزیم آسکوربات پراکسیداز، فعالیت آنزیم کاتالاز، فعالیت آنزیم فنیل آلانین آمونیالیاز، و در نهایت، عملکرد برگ گیاه حنا ارزیابی شدند. نتایج نشان داد که تنش خشکی باعث کاهش صفاتی مانند ارتفاع بوته (37.04 درصد) و شاخص سطح برگ (50.14 درصد) گیاه حنا ‌شد. همچنین، تنش خشکی سبب افزایش صفاتی نظیر غلظت مالون دی‌آلدئید (45.94 درصد)، فعالیت آنزیم کاتالاز (57.14 درصد) و فعالیت آنزیم فنیل آلانین آمونیالیاز (57.14 درصد) گردید. استفاده از ترکیب اسید سالیسیلیک و سدیم نیتروپروساید منجر به افزایش صفاتی نظیر شاخص سطح برگ (13.44 درصد)، فعالیت آنزیم آسکوربات پراکسیداز (13.63 درصد)، فعالیت آنزیم کاتالاز (18.75 درصد)، و فعالیت آنزیم فنیل آلانین آمونیالیاز (25 درصد) شد. بیشترین عملکرد برگ در تیمار محلول‌پاشی ترکیب اسید سالیسیلیک و سدیم نیتروپروساید به‌میزان 2984.44 کیلوگرم در هکتار به‌دست آمد.

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