Iranian Journal of Field Crops Research

Iranian Journal of Field Crops Research

The Response of Some Growth Indices, Yield and Forage Quality of Two Varieties of Quinoa (Chenopodium quinoa Willd) to Different Levels of Plant Density in the ‌Bavi region, Khuzestan Province

Document Type : Research Article

Authors
Department of Plant Production and Genetics, Faculty of Agriculture, University of Agricultural Sciences and Natural Resources of Khuzestan, Mollasani, Iran
Abstract
Introduction
Quinoa (Chenopodium quinoa Willd) is an annual plant of the legume family, native to Latin America. In addition to its high nutritional value, this plant has a remarkable ability to tolerate a variety of abiotic stresses, including drought, salinity, and cold, as well as to grow on marginal lands. Due to its relative tolerance to drought and salinity stresses, extensive genetic diversity, and ability to adapt to different climatic conditions and high water use efficiency, it is considered a suitable plant for the exploitation of limited water resources and saline soils. In terms of nutritional value, quinoa seeds, the main product of this plant, contain between 14 and 20% protein and are rich sources of essential amino acids such as lysine and methionine, substances that are found in low amounts in most cereals.

Materials and Methods
In order to investigate the effect of different plant densities and cultivars of quinoa on growth, physiology and forage yield, a field experiment was conducted as a factorial experiment in a randomized complete block design with four replications in the fall and winter of the 2023-2024 crop year at the research farm of Agricultural Sciences and Natural Resources University of Khuzestan. Experimental factors included plant density at three levels (30, 50 and 70 plants m-2) and quinoa cultivars (Rahmat and Sadooq). The evaluated traits were chlorophyll a, b, carotenoid, spad number, leaf area index, forage protein percentage, forage protein yield and forage yield.

Results and Discussion
In this study, neither planting density nor cultivar had a significant effect on chlorophyll a, chlorophyll b, carotenoid content, or SPAD values. However, analysis of variance showed that planting density significantly affected leaf area index, forage protein concentration, and forage protein yield, whereas cultivar significantly affected forage protein yield and dry forage yield. In addition, the interaction between planting density and cultivar had a significant effect on forage protein concentration and forage protein yield. The results showed that the highest and lowest of leaf area index with an average of (3.87) and (3.11) were observed in the density treatment of 70 and 30 plants per square meter, respectively. Also, the highest percentage of forage protein (6.35 and 6.03 percent, respectively) was observed in the density of 30 plants per square meter and the lowest were (4.10 and 4.88 percent, respectively) in the density of 70 plants per square meter in two varieties of Sadooq and Rahmat. The highest forage protein yield (1163 kg ha-1) was observed at densities of 30 plants per square meter, in Sadooq cultivar and the lowest (540 kg ha-1) was obtained at densities of 70 plants per square meter, in Rahmat cultivar. Also, the highest dry forage yield with an average of 18259 kg ha-1 was observed in the Sadooq cultivar and the lowest dry forage yield with an average of 10633 kg ha-1 was observed in the Rahmat cultivar. Therefore, the significant difference between the two studied cultivars were present.

Conclusion
Increasing plant density to the optimum level, without creating intense intraspecific competition, by making optimal use of environmental resources such as light, water, and nutrients, improved leaf area index and increased forage yield in quinoa. The results showed that choosing the right cultivar plays an important role in forage yield, especially through its effect on maturity time and weed resistance. Significant differences in forage yield were observed among the evaluated cultivars, with Sadooq producing the highest forage yield. These findings indicate that selecting an appropriate cultivar in combination with an optimal planting density can be an effective strategy for improving quinoa forage production.
Keywords
Subjects

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1.          Alizadeh, F., Zaefarian, F., Torabi, B., & Abbasi, R. (2021). Investigation of the effect of plant density on growth indices of different cultivars of rapeseed (Brassica napus L.) in Mazandaran climatic conditions. Journal of Crop Production14(3), 107-124. (in Persian). https://doi.org/10.22069/ejcp.2022.18883.2409
2.          Antonietta, M., Fanello, D. D., Acciaresi, H. A., & Guiamet, J. J. (2014). Senescence and yield responses to plant density in stay green and earlier-senescing maize hybrids from Argentina. Field Crops Research, 155(1), 111-119. https://doi.org/10.1016/j.fcr.2013.09.016
3.          Arnon, A. N. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23(1), 112-121.
4.          Ashraf, M. Y., Azmi, A. R., Khan, A. H., & Ala, S. A. (1994). Effect of water stress on total phenols, peroxidase activity and chlorophyll content in wheat (Triticum aestivum L.). Acta Physiologiae Plantarum, 16(3), 185-191.
5.          Baskota, S., & Islam, A. (2017). Evaluation of forage nutritive value of quinoa cultivars. Field Days Bulletin, LREC Long Reports.
6.          Bertero, H. D., & Ruiz, R. A. (2008). Determination of seed number in sea level quinoa (Chenopodium quinoa Willd.) cultivars. European Journal of Agronomy, 28(3), 186-194. https://doi.org/10.1016/j.eja.2007.07.002
7.          Bhargava, A., Shukla, S., & Ohri, D. (2007). Effect of sowing dates and row spacings on yield and quality components of quinoa (Chenopodium quinoa) leaves. Indian Journal of Agricultural Sciences, 77(11), 748-751.
8.          Eisa, S. S., Abd El Samad, E. H., Hussin, S. A., Ali, E. A., Ebrahim, M., González, J. A., & Abdel-Ati, A. A. (2018). Quinoa in Egypt-plant density effects on seed yield and nutritional quality in marginal regions. Journal of Applied Sciences, 8(2), 515-522.
9.          Erazzú, L. E., González, J. A., Buedo, S. E., & Prado, F. E. (2016). Effects of sowing density on Chenopodium quinoa (quinoa). Incidence on morphological aspects and grain yield in Var. CICA growing in Amaicha del Valle (Tucumán, Argentina). Journal Lilloa Facultad de Agronomia Y Zootecnia, 53(1), 12-22.
10.       Eslami, M., Salek Mearaji, H., Jamshidi, D., Taghavi, A., & Adinevand, I. (2024). Investigation the effect of planting distance on morphological traits, yield and yield components and quality of grain quinoa (Chenopodium quinoa Willd.). Crop Science Research in Arid Regions6(2), 381-394. (In Persian). https://doi.org/10.22034/csrar.2024.403211.1349
11.       FAO. )2023(. FAO Statistic Service, [Online]. Available at: www.FAO.org/crop/statistics.
12.       Forghani, A., Khoda Bandeh, N., Habibi, D., & Bankehsaz, A. (2010). Reaction of chlorophylls a and b, proline and yield of corn (SC704) to light stress and different density levels. Journal of Crop Production Research (Environmental Stresses in Plant Sciences), 2(1), 29-37. (in Persian).
13.       García, M., Condori, B., & Del Castillo, C. D. (2015). Agroecological and agronomic cultural practices of quinoa in South America. Quinoa: Improvement and Sustainable Production, 3(2), 25-46. https://doi.org/10.1002/9781118628041.ch3
14.       Iqbal, S., Basra, S. M., Afzal, I., Wahid, A., Saddiq, M. S., Hafeez, M. B., & Jacobsen, S. E. (2019). Yield potential and salt tolerance of quinoa on salt‐degraded soils of Pakistan. Journal of Agronomy and Crop Science205(1), 13-21. https://doi.org/10.1111/jac.12290
15.       Jacobsen, S. E. (2011). The situation for quinoa and its production in southern Bolivia: From economic success to environmental disaster. Journal of Agronomy and Crop Science, 197(5), 390-399. https://doi.org/10.1111/j.1439-037X.2011.00475.x
16.       Karami, R., Farajee, H., Movahedi Dehnavi, M., & Khoshroo, A. (2020). Interaction of nitrogen and plant density on growth and yield of quinoa (Chenopodium quinoa Willd.). Journal of Crop Production13(1), 111-124. (in Persian). https://doi.org/10.22069/ejcp.2020.17603.2296
17.       Nabipour, Z., Zamani, G., & Gheisari, Y. (2023). Effect of different levels of nitrogen application on yield and yield components of quinoa (Chenopodium quinoa L.) under different planting densities. Applied Soil Research10(4), 61-75. (In Persian).
18.       Najafinezhad, H., Tahmasebi Sarvestani, Z. A., & Modarres Sanavy, S. A. M. (2014). Evaluation of forage yield and quality of maize and sorghum under water stress and application of barley residue, zeolite and super absorbent polymer in terms of minimum tillage. 1st Intemational and 13th Iranian Crop Science Congress 3rd Iranian Seed Science and Technology Conference. Seed and Plant Improvement Institute, Karaj, Iran (in Persian).
19.       Najafinezhad, H., Shakeri, P., & Amirpour Robat, M. (2021). Effect of planting date and plant density on forage yield and quality of quinoa (Chenopodium quinoa willd.) varieties in cold temperate region of Kerman province in Iran. Seed and Plant Journal36(4), 439-460. (in Persian). https://doi.org/10.22092/sppi.2021.123894
20.       Najafinezhad, H., Koohi, N., & Darvishi, D. (2022). Evaluation of grain yield and quality of quinoa cultivars as affected by planting date and plant density in Jupar region of Kerman. Iranian Journal of Field Crop Science53(1), 113-129. (In Persian). https://doi.org/10.22059/IJFCS.2021.311672.654761
21.       Papastylianou, P., Kakabouki, I., Tsiplakou, E., Travlos, I., Bilalis, D., Hela, D., Chachalis, D., Anogiatis, G., & Zervas, G. (2014). Effect of fertilization on yield and quality of biomass of quinoa (Chenopodium quinoa Willd.) and green amaranth (Amaranthus retroflexus L.). Bulletin Uasvm Horticulture, 71(2), 288-292. https://doi.org/10.15835/buasvmcn-hort:10411
22.       Phillips, K. M., Haytowitz, D. B., & Pehrsson, P. R. (2019). Implications of two different methods for analyzing total dietary fiber in foods for food composition databases. Journal of Food Composition and Analysis, 84(3), 1-70. https://doi.org/10.1016/j.jfca.2019.103253
23.       Präger, A., Munz, S., Nkebiwe, P. M., Mast, B., & Graeff-Hönninger, S. (2018). Yield and quality characteristics of different quinoa (Chenopodium quinoa Willd.) cultivars grown under field conditions in Southwestern Germany. Agronomy, 8(10), 1-19. https://doi.org/10.3390/agronomy8100197
24.       Saberi, A. (2024). The effects of plant density and irrigation management on forage production of quinoa and forage sorghum. Journal of Crop Production17(1), 82-98. (In Persian). https://doi.org/10.22069/ejcp.2024.21796.2602
25.       Soltani, A. (2009). Mathematical Modeling in Field Crops. Mashhad Scientific Press, Mashhad, Iran. 175 p. (in Persian).
26.       Spehar, C. R., & Rocha, J. D. S. (2009). Effect of sowing density on plant growth and development of quinoa, genotype 4.5, in the Brazilian savannah highlands. Journal of Bioscience, 25(3), 53-58.
27.       Vahedi, M. R., Tohidi Nejad, E., & Pasandi Pour, A. (2022). Evaluation of yield and yield components of quinoa (Chenopodium quinoa Willd.) in as affected by different planting densities. Journal of Crop Ecophysiology (Agriculture Science), 15(4), 593-608. (In Persian).
28.       Van Minh, N., Hoang, D. T., Van Loc, N., & Long, N. V. (2020). Effects of plant density on growth, yield and seed quality of quinoa genotypes under rain-fed conditions on red basalt soil regions. Australian Journal of Crop Science, 14(12), 1977-1982. https://doi.org/10.21475/ajcs.20.14.12.2849
29.       Van Soest, P. V., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
30.       Waghorn, G. C., Burke, J. L., & Kolver, E. S. (2007). Principles of feeding value. New Zealand Society of Animal Production: Hamilton, New Zealand, 42(3), 35-59.
31.       Zafari Ghalehrodkhani, B., Soltani, A., Zeinali, E., Kamkar, B., & Firozfard, M. (2017). Effect of plant density on allometric relationships between leaf area and vegetative traits of wheat. Iranian Journal of Field Crops Research15(3), 546-558. (In Persian). https://doi.org/10.22067/gsc.v15i3.46036
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  • Receive Date 04 November 2025
  • Revise Date 13 February 2026
  • Accept Date 17 February 2026
  • First Publish Date 12 May 2026