The Effect of Combined Use of Nitrogen Fertilizer and Seaweed on Yield Components and Grain Yield of Rice (Oryza sativa L.) Variety Hashemi

Document Type : Research Article

Authors

1 Lahijan Branch, Islamic Azad University, Lahijan, Iran

2 Department of Agriculture, Lahijan Branch, Islamic Azad University, Lahijan, Iran

Abstract

Introduction
Rice (Oryza sativa L.) is the staple food of half of the world's population, providing 21% of the energy and 15% of the protein of residents of rice-growing regions. Compared to other essential elements, rice needs more nitrogen. Therefore, for higher plant growth and productivity, nitrogen is used excessively. Nitrogen plays a vital role in the growth and development of rice and is an important part of enzymes, chlorophyll, nucleic acids, storage proteins, cell walls, and other cellular components. Excessive nitrogen application has resulted in many adverse effects such as increased nitrogen oxide emissions, reduced nitrogen use efficiency (NUE), and poor economic returns. Combining chemical fertilizers with biological and organic fertilizers is one of the ways to reduce the excessive consumption of chemical fertilizers. The use of seaweed as fertilizer in recent years has led to an increase in the quantity and quality of agricultural products due to its bioactive compounds. The purpose of this experiment was to investigate the effect of different levels of nitrogen fertilizer and seaweed foliar application on yield components, biomass, and grain yield of Hashemi rice cultivar in the Rudsar region.
Materials and Methods
In order to determine the appropriate level of nitrogen fertilizer and seaweed extract on the yield and yield components of Hashemi cultivar, an experiment was conducted, in Kishakjan village, Rudsar city, in Guilan province in 2022 and 2023. The experiment used the split plots in the form of randomized complete block design with three replications, the main factor being nitrogen fertilizer from the urea source at five levels (zero (control: without fertilizer use), 25, 50, 75 and 100 percent of nitrogen needed by the plant) and the sub factor of foliar spraying of fertilizer containing seaweed extract at four levels (zero (control: without seaweed use), 0.5, 1 and 1.5 liters per hectare) were considered.
Results and Discussion
The results showed that the application of nitrogen fertilizer levels and seaweed foliar application was significant on the investigated characteristics. So that the highest number of chlorophyll content (35), spike length (29.3 cm), thousand grain weight (26.3 g), grain yield (4014 kg ha-1), biological yield (7690.8 kg ha-1) and the harvest index (59.5%) was obtained in the application of 75% nitrogen fertilizer. In addition, the highest amount of the characteristics expressed in foliar spraying of seaweed fertilizer with a concentration of 1 lit ha-1 was obtained, which was not significantly different from the concentration of 1.5 1 lit h-1. The results of the interaction of experimental treatments also showed that the highest plant height (150 cm), the number of spike (29), the number of full seeds (132.4), respectively, were related to the application of 100% nitrogen fertilizer* 1 lit ha-1 of seaweed and 75% nitrogen fertilizer* 1 lit ha-1 of seaweed. The lowest number of unfilled seeds (4.2) and percentage of unfilled seeds (3.5) were recorded for the application of 75% nitrogen fertilizer*no use of seaweed fertilizer and 75% nitrogen fertilizer*1 lit ha-1of seaweed fertilizer, respectively.
Conclusion
Nowadays, due to the high environmental and economic costs of chemical fertilizers, the use of fertilizers of biological and organic origin has received more attention than ever before. Based on the results of this study, foliar application of seaweed fertilizer in combination with nitrogen fertilizer significantly increased plant height, number of panicles, number of grain per panicle, and number of full grain per panicle. Nitrogen fertilizer application and foliar application of seaweed extract also increased chlorophyll content, panicle length, and 1000-grain weight, improving paddy yield and biological yield of Hashemi rice cultivar. In general, foliar application of one liter per hectare of seaweed and use of 50 and 75% nitrogen fertilizer was the best treatment to increase yield and yield components of Hashemi variety in the study region.

Keywords

Main Subjects


©2025 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

  1. Aminpanah, H., & Abassian, A. (2016). Effect of crop rotation, Azotobacter chroococcum inoculation and nitrogen rate on rice (Oryza sativa ) paddy yield. Crop Production, 9(3), 211-230. https://doi.org/10.22069/EJCP.2016.10291.1804
  2. Amiri, E., Razavipour, T., Farid, A., & Bannayan, M. (2011). Effects of crop density and irrigation management on water productivity of rice production in Northern Iran: Field and modeling approach. Communications in Soil Science and Plant Analysis, 42(17), 2085-2099. https://doi.org/10.1080/00103624.2011.596238
  3. Ashouri, M. (2012). The effect of water saving irrigation and nitrogen fertilizer on rice production in paddy fields of Iran. International Journal of Bioscience, Biochemistry and Bioinformatics, 2(1), 56-59. https://doi.org/7763/IJBBB.2012.V2.70
  4. Baňoc, D. M. (2022). Ratooning response of lowland rice (Oryza sativa) to foliar application of seaweed extracts grown under high maximum temperatures. SVU-International Journal of Agricultural Sciences, 4(3), 68-78. https://doi.org/10.21608/SVUIJAS.2022.133392.1205
  5. De Carvalho, M. E. A., De Camargo, P. R., Gallo, L. A., & Junior, M. V. C. F. (2014). Seaweed extract provides development and production of wheat. Agrarian, 7(23), 166-170. https://doi.org/15281/zenodo.51607
  6. Deepana, P., Bama, K. S., Santhy, P., & Devi, T. S. (2021). Effect of seaweed extract on rice (Oryza sativa ADT53) productivity and soil fertility in Cauvery delta zone of Tamil Nadu, India. Journal of Applied and Natural Science, 13(3), 1111-1120. https://doi.org/10.31018/jans.v13i3.2906
  7. Food and Agriculture Organization (FAO). (2018) .
  8. Fageria, N. K., Dos Santos, A. B., & De Oliveira, J. P. (2013). Nitrogen-use efficiency in lowland rice genotypes under field conditions. Communications in Soil Science and Plant Analysis, 44(17), 2497-2506. https://doi.org/10.1080/00103624.2013.812732
  9. Fei, L., Pan, Y., Ma, H., Guo, R., Wang, M., Ling, N., Shen, Q., & Guo, Sh. (2024). Optimal organic-inorganic fertilization increases rice yield through source-sink balance during grain filling. Field Crops Research, 308, 1-12. https://doi.org/10.1016/j.fcr.2024.109285
  10. Fu, Y., Yang, G., Pu, R., Li, Z., Li, H., Xu, X., & Zhao, C. (2021). An overview of crop nitrogen status assessment using hyperspectral remote sensing: Current status and perspectives. European Journal of Agronomy, 124, 126241. https://doi.org/10.1016/j.eja.2021.126241
  11. Ghosh, M., Swain, D. K., Jha, M. K., Tewari, V. K., & Bohra, A. (2020). Optimizing chlorophyll meter (SPAD) reading to allow efficient nitrogen use in rice and wheat under rice-wheat cropping system in eastern India. Plant Production Science, 23(3), 270-285. https://doi.org/10.1080/1343943X.2020.1717970
  12. Goyal, P., & Thind, S. K. (2016). Photosynthetic attributes and carbohydrates in relation to yield of aerobic rice as influenced by nitrogen and sea weed extract application. Agricultural Research Journal, 53(4), 1-13.
  13. Halvorson, A. D., Snyder, C. S., Blaylock, A. D., & Del Grosso, S. J. (2014). Enhanced‐efficiency nitrogen fertilizers: Potential role in nitrous oxide emission mitigation. Agronomy Journal, 106(2), 715-722. https://doi.org/10.2134/agronj2013.0081
  14. Hatamifar, B., Ashoury, M., Shokri-Vahed, H., & Shahin-Rokhsar, P. (2013). Effects of irrigation and various rates of nitrogen and potassium on yield and yield components of rice plant (Oryza sativa). Persian Gulf Crop Protection, 2(2), 19-25.
  15. Hernández-Herrera, R. M., Santacruz-Ruvalcaba, F., Ruiz-López, M. A., Norrie, J., & Hernández-Carmona, G. (2014). Effect of liquid seaweed extracts on growth of tomato seedlings (Solanum lycopersicum). Journal of Applied Phycology, 26, 619-628.
  16. Kumar, A., Singh, K., Verma, P., Singh, O., Panwar, A., Singh, T., & Raliya, R. (2022). Effect of nitrogen and zinc nanofertilizer with the organic farming practices on cereal and oil seed crops. Scientific Reports, 12(1), 6938. https://doi.org/10.1038/s41598-022-10843-3
  17. Latique, S., Chernane, H., Mansori, M., & El Kaoua, (2013). Seaweed liquid fertilizer effect on physiological and biochemical parameters of bean plant (Phaesolus vulgaris variety Paulista) under hydroponic system. European Scientific Journal, 9(30). 174-191.
  18. Liu, K., Li, T., Chen, Y., Huang, J., Qiu, Y., Li, S., & Yang, J. (2020). Effects of root morphology and physiology on the formation and regulation of large panicles in rice. Field Crops Research, 258, 107946. https://doi.org/10.1016/j.fcr.2020.107946
  19. Luo, L., Zhang, Y., & Xu, G. (2020). How does nitrogen shape plant architecture? Journal of Experimental Botany, 71(15), 4415-4427. https://doi.org/10.1093/jxb/eraa187
  20. MacKinnon, S. L., Hiltz, D., Ugarte, R., & Craft, C. A. (2010). Improved methods of analysis for betaines in Ascophyllum nodosum and its commercial seaweed extracts. Journal of Applied Phycology, 22, 489-494. https://doi.org/10.1007/s10811-009-9483-0
  21. Nayak, P., Biswas, S., & Dutta, D. (2020). Effect of seaweed extracts on growth, yield and economics of kharif rice (Oryza sativa). Journal of Pharmacognosy and Phytochemistry, 9(3), 247-253. https://doi.org/10.22271/phyto.2020.v9.i3d.11269
  22. Nedunchezhiyan, M., & Laxminarayan, K. (2011). Site-specific nutrient management for rice. Orissa Review, 2, 62-64.
  23. Noor, H., Ding, P., Ren, A., Sun, M., & Gao, Z. (2023) Effects of nitrogen fertilizer on photosynthetic characteristics and yield. Agronomy, 13(1550), 1-20. https://doi.org/13390/agronomy13061550
  24. Nowak, R., Szczepanek, M., Kobus-Cisowska, J., Stuper-Szablewska, K., Graczyk, R., & Blaszczyk, K. (2024). Relationships between photosynthetic efficiency and grain antioxidant content of barley genotypes under increasing nitrogen rates. Agriculture, 14(1913), 1-21. https://doi.org/13390/agriculture14111913
  25. Patane, P., & Vibhute, A. (2014). Chlorophyll and nitrogen estimation techniques: A review. International Journal of Engineering Research and Reviews, 2(4), 33-41.
  26. Pawar, S. Y., Radhakrishnan, V. V., & Mohanan, K. V. (2016). The importance of optimum tillering in rice-an overview. South Indian Journal of Biological Sciences, 2(1), 125-127.
  27. Rahman, M. R. (2015). Effectiveness of nitrogen and potassium fertilizer application on lodging habit and yield attributes of Aman rice in Ganges Tidal Flood plain. International Journal of Business, Social and Scientific Research, 3(1), 1-12.
  28. Rajaie, M. (2022). Improving yield, yield components and the absorption of nutrients of wheat by growth stimulants under normal irrigation and drought stress, Iranian Journal of Field Crops Research, 20(2), 147-162. (in Persien with English Abstarct). https://doi.org/22067/JCESC.2022.72226.1083
  29. Ren, Ch., Zhang, X., Reis, S., & Gu, B. (2022). Socioeconomic barriers of nitrogen management for agricultural and environmental sustainability. Agriculture, Ecosystems & Environment, 333, 1-12. https://doi.org/1016/j.agee.2022.107950
  30. Sasikala, M., Indumathi, E., Radhika, S., & Sasireka, R. (2016). Effect of seaweed extract (Sargassum tenerrimum) on seed germination and growth of tomato plant. International Journal of ChemTech Research, 9(09), 285-293.
  31. Sheikhnazari, S., Niknezhad, Y., Fallah, H., & Barari Tari, D. (2022). Effect of application of nitrogen doses with biochar and zinc nanoparticles on quantitative and qualitative characteristic of rice (Oryza sativa ), Iranian Journal of Field Crops Research, 20(3), 349-361. (in Persien with English Abstract). https://doi.org/10.22067/JCESC.2022.75649.1150
  32. Shukla, P. S., Mantin, E. G., Adil, M., Bajpai, S., Critchley, A. T., & Prithiviraj, B. (2019). Ascophyllum nodosum-based biostimulants: Sustainable applications in agriculture for the stimulation of plant growth, stress tolerance, and disease management. Frontiers in Plant Science, 10, 462648. https://doi.org/10.3389/fpls.2019.00655
  33. Siavoshi, M., Nasiri, A., & Laware, S. L. (2011). Effect of organic fertilizer on growth and yield components in rice (Oryza sativa). Journal of Agricultural Science, 3(3), 217-221. https://doi.org/10.5539/jas.v3n3p217
  34. Sivakamipriya, J., Suresh, S., Manikandan, K., & Ramesh, P. T. (2022). Effect of water soluble fertilizer, micronutrients, humic acid and seaweed extract on growth and yield of rice. Biological Forum – An International Journal, 14(2), 493-498.
  35. Sridhar, S., & Rengasamy, R. (2010). Studies on the effect of seaweed liquid fertilizer on the flowering plant Tagetes erecta in field trial. Advances in Bioresearch, 1(2), 29-34.
  36. Sunarpi, H., Nikmatullah, A., Sunarwidhi, A. L., Jihadi, A., Ilhami, B. T. K., Ambana, Y., & Prasedya, E. S. (2021, March). Combination of inorganic and organic fertilizer in rice plants (Oryza sativa L.) in screen houses. In IOP Conference Series: Earth and Environmental Science (Vol. 712, No. 1, p. 012035). IOP Publishing. https://doi.org/1088/1755-1315/712/1/012035
  37. Szczepanek, M., Wszelaczynska, E., & Poberezny, J. (2018). Effect of seaweed biostimulant application in spring wheat. Agro Life Scientific Journal, 7(1), 1-7.
  38. Wang, N., Fu, F., Wang, H., Wang, P., He, S., Shao, H., & Zhang, X. (2021). Effects of irrigation and nitrogen on chlorophyll content, dry matter and nitrogen accumulation in sugar beet (Beta vulgaris). Scientific Reports, 11(1), 1-9. https://doi.org/10.1038/s41598-021-95792-z
  39. Wang, Y., Ren, T., Lu, J., Ming, R., Li, P., Hussain, S., & Li, X. (2016). Heterogeneity in rice tillers yield associated with tillers formation and nitrogen fertilizer. Agronomy Journal, 108(4), 1717-1725. https://doi.org/10.2134/agronj2015.0587
  40. Wen ZhiHui, W. Z., Shen JianBo, S. J., Blackwell, M., Li HaiGang, L. H., Zhao BingQiang, Z. B., & Yuan HuiMin, Y. H. (2016). Combined applications of nitrogen and phosphorus fertilizers with manure increase maize yield and nutrient uptake via stimulating root growth in a long-term experiment. Pedospher, 26(1), 62-73. https://doi.org/10.1016/S1002-0160(15)60023-6
  41. Xie, H., Wu, K., Iqbal, A., Ali, I., He, L., Ullah, S., & Jiang, L. (2021). Synthetic nitrogen coupled with seaweed extract and microbial inoculants improves rice (Oryza sativa) production under a dual cropping system. Italian Journal of Agronomy, 16(2), 1-10. https://doi.org/10.4081/ija.2021.1800
  42. Xu, G., Fan, X., & Miller, A. J. (2012). Plant nitrogen assimilation and use efficiency. Annual Review of Plant Biology, 63, 153-182. https://doi.org/1146/annurev-arplant-042811-105532
  43. Yang JianChang, Y. J., Zhang Hao, Z. H., & Zhang JianHua, Z. J. (2012). Root morphology and physiology in relation to the yield formation of rice. Journal of Integrative Agriculture, 11(6), 920-926. https://doi.org/10.1016/S2095-3119(12)60082-3
  44. Zayed, B. A., Elkhoby, W. M., Salem, A. K., Ceesay, M., & Uphoff, N. T. (2013). Effect of integrated nitrogen fertilizer on rice productivity and soil fertility under saline soil conditions. Journal of Plant Biology Research, 2(1), 14-24.
  45. Zayed, B. A., Salem, A. K. M., & El Sharkawy, H. M. (2011). Effect of different micronutrient treatments on rice (Oriza sativa) growth and yield under saline soil conditions. World Journal of Agricultural Sciences, 7(2), 179-184.
  46. Zhang, H., Liu, H., Hou, D., Zhou, Y., Liu, M., Wang, Z., & Yang, J. (2019). The effect of integrative crop management on root growth and methane emission of paddy rice. The Crop Journal, 7(4), 444-457. https://doi.org/10.1016/j.cj.2018.12.011
  47. Zhang, W., Wu, L., Wu, X., Ding, Y., Li, G., Li, J., & Wang, S. (2016). Lodging resistance of japonica rice (Oryza Sativa): Morphological and anatomical traits due to top-dressing nitrogen application rates. Rice, 9, 1-11. https://doi.org/10.1186/s12284-016-0103-8
  48. Zodape, S. T., Mukhopadhyay, S., Eswaran, K., Reddy, M. P., & Chikara, J. (2010). Enhanced yield and nutritional quality in green gram (Phaseolus radiata) treated with seaweed (Kappaphycus alvarezii) extract. Journal of Scientific and Industrial Research, 69, 468-471.
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Volume 23, Issue 3 - Serial Number 79
September 2025
Pages 271-284
  • Receive Date: 13 August 2024
  • Revise Date: 09 November 2024
  • Accept Date: 19 November 2024
  • First Publish Date: 12 April 2025