The Effect of Biofertilizers and Winter Cover Crops on Essential Oil Production and Some Agroecological Characteristics of Basil (Ocimum basilicum L.)

Document Type : Research Article

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Abstract

In searching for new strategies of medicinal plant production with high yield but without undesirable compounds or effects, it is important to investigate unconventional alternatives such as application of PGPR and cover crops cultivation. This experiment was conducted in a split plots arrangement with two factors based on randomized complete block design with three replications during years 2009-10, at Research Farm of Ferdowsi University of Mashhad. Cultivation and no cultivation of cover crops in autumn assigned to the main plots. The sub factor was biofertilizer application with four levels, included 1-Nitroxin (containing Azotobacter spp. and Azospirillum spp.), 2-Biophosphorous (Bacillus sp. and Pseudomonas sp.), 3-Nitroxin + Biophosphorous and 4-Control. During growing season plants were harvested by three cuts. Results showed that total shoots dry weight, leaves yield and LAI in plants under no cover crop cultivation had a significant advantage. Biofertilizers increased most characteristics e.g. fresh and dry total shoot yield, dry leaves and LAI. The interaction between fertilizer and cover crop was significant, as the highest yield of fresh shoots was observed in mix of nitroxin and biophosphorous with no cover crop, the highest and the lowest of leaf and green area index were obtained in plants treated by nitroxin without cover crop and biophosphorous with cover crop, respectively. Plants harvested in cut 3 had the lowest LAI and other two cuts had no significant difference concerning this trait. The highest and the lowest fresh and dry shoot yield were observed in cut 2 and 1, respectively. The most essential oil yield was in cut 2 and 3 (without significant difference) and cut 1 was the lowest. The results showed that the interaction between biofertilizers and no cover crop cultivation was significant, as use of the biofertilizers especially nitroxin and biophosphorous in no cover crop condition enhanced the most characteristics of basil in an ecological production system framework.

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