Effect of Hexaconazole and Penconazole on Kochia (Kochia scoparia) Freezing Tolerance

Document Type : Research Article

Authors

• A Kamandy
• A Nezami
• M Kafi
• J Nabati

Ferdowsi University of Mashhad

10.22067/gsc.v14i1.21453

Abstract

Introduction
Cold and freezing are the most important limiting factors on development of sensitive plants. When the ambient temperature deviates from optimal, physiological, and biochemical, metabolic and molecular changes will occur within plants. This is an effort of plants to maximize growth and developmental processes and to maintain cellular homeostasis during such adverse conditions. At the extremes of the natural temperature range of plant, the degree of physiological, cellular, metabolic and molecular dysfunction becomes so severe that it leads to death. Triazoles are the most potent groups of growth retardants with multiple effects. Plant growing with paclobutrazol generally has little effect on rates of net photosynthetic rate. However, because the compound reduces leaf area, net photosynthesis on a per plant basis is probably reduced. They have exhibited growth regulating, fungicidal, herbicidal, apicidal and antibacterial activities. More recently, it was found that triazole compounds are able to increase tolerance of plants to cold and freezing stress. Kochia (Kochia scoparia (L.) Schrad.), an out crossing species whose pollen move between plants in windy areas, has recently been considered as a forage or fodder crop in marginal lands. Steppuhn and Wall (1993) claimed that Kochia offers great potential as a crop that can be grown on saline soils, yielding fodder in quantities approaching that produced by alfalfa (Medicago sativa L.). Shamsutdinov et al. (1996) also reported more than 15 Mg ha-1 dry-matter production for Kochia under saline conditions and concluded that it is a good candidate for forage hay. Thus, the aim of the present study was to determine whether the triazole compounds could increase tolerance of kochia against freezing stress.
Materials and Methods
An experiment was carried out with hexaconazole and penconazole in 0, 10 and 20 mg.L-1 and freezing temperatures 0, -2, -4, -6 and -8 degree centigrade in factorial based on CRBD with three replications at research greenhouse of college of agriculture, Ferdowsi University of Mashhad in 2009. Plants were kept in natural condition until three weeks after planting, which in this stage they had three leaves, and then treated with fungicide. After 24 hours plants were frozen in thermo gradient freezer, in the dark. At first, the temperature of thermo gradient freezer was five degrees centigrade, and then temperature decreased two degree centigrade per hour. In order to make ice nucleation in plant at -3-degree centigrade ice nucleation active bacteria was sprayed. Plants were keeping an hour in each temperature treatment. In order to balance the ambient temperature, the plants are kept at the desired temperature for one hour and then the pots transport in a cold chamber at a temperature of 5 ± 2°C for 24 hours. The cell membrane stability was measured through electrolyte leakage (EL) and the lethal temperature 50 (LT50el) (according to El) also were determined in the youngest developed leaf from each plant. The electrolyte leakage in the solution was measured after 24 h of floating at room temperature using a conductivity meter. Total conductivity was obtained after keeping the flasks in an oven (75°C) for 90 minutes. Results were expressed as percentage of total conductivity. Survival percentage, plant height, dry weight and (LT50su) (according to survival) were determined after three weeks recovery in the natural condition. The data were analyzed statistically using a two-way ANOVA, applied to the various measured and calculated parameters, followed by a Duncan test for mean comparison between treatments at a 95 % confidence level by MSTAT-C program.
Results and Discussion
The results showed that increase of fungicide concentration by 10 and 20 mg.L-1 higher than control decreased electrolyte leakage but, electrolyte leakage build-up with decrease freezing temperature. Fungicide application increased -2 °C freezing tolerance in kochia. Increasing fungicide concentration in 10 and 20 mg.L-1 than control causes decreased LT50el from -5.9 to -7.2. Application of triazoles responsible of reduced electrolyte leakage but they did not effect on survival. The use of different concentrations of fungicides hexaconazole and penconazole of photosynthetic pigments in Kochia showed no significant effect. However, the concentration of chlorophyll a and b in the use of fungicides hexaconazole was more than penconazole.
Conclusions
Triazole application initially had a positive effect on the reduction of electrolyte leakage, but survival and re-growth did not affect. Generally, the use of Triazoles compounds prior to stresses such as cold, could expect the plants can tolerate oxidative stress, which usually occurs after cold stress.

Keywords

• Cold
• Electrolyte leakage
• Lethal temperature 50
• Triazole

20.1001.1.20081472.1395.14.1.2.9

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References

1. Abdul Jaleel, C., Gopi, R., and Panneerselvam, R. 2008. Growth and photosynthetic pigments responses of two varieties of Catharanthus roseus to triadimefon treatment. Comptes Rendus Biologies 331: 272-277.

2. Abdul Jaleel, C., Manivannan, P., Sankar, B., Kishorekumar, C. A., Gopi, R., Somasundaram, R., and Panneerselvam, R. 2007. Induction of drought stress tolerance by ketoconazole in Catharanthus roseus is mediated by enhanced antioxidant potentials and secondary metabolite accumulation. Colloids and Surfaces B: Biointerfaces 60: 201-206.

3. Azizi, H., Nezami, A., Nasiri Mahalati, M., and Khazaie, H. R. 2007. Evaluation of cold tolerance in wheat (Triticum aestivum L.) cultivars under controlled conditions. Iranian Journal of Field Crops Research 1: 1-12. (in Persian with English abstract).

4. Bisht, R., Singariya, P., Mathur, N., and Bohra, S. P. 2007. Triazoles: Their effects on net photosynthetic rate, transpiration rate and stomatal resistance in Setaria italica plants grown in vivo. Asian Journal of Experimental Sciences 21: 271-276.

5. Blum, A. 1988. Plant breeding for stress environments. CRC Press, Inc., Boca Raton, FL.

6. Boughanmi, N., Michonneau, P., Verdus, M. C., Piton, F., Ferjani, E., Bizid, E., and Fleurat-Lessard, P. 2003. Structural changes induced by NaCl in companion and transfer cells of Medicago sativa blades. Protoplasma 220: 179-187.

7. Dubey, R. S. 1997. Photosynthesis in plants under stressful conditions. In: Pessarakli. M (Eds.) Hand book of Photosynthesis. Marcel Dekker Inc, New York.

8. Eugenia, M., Nunes, S., and Ray Smith, G. 2003. Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. Crop Science 43: 1349-1357.

9. Fletcher, R., and Hofstra, G. 1988. Triazoles as potential plant protectants. In: Sterol synthesis inhibitors in plant protection. Eds D. Berg, M. Plempel, Cambridge, Ellis Horwood Ltd, 321-331.

10. Fletcher, R. A., Gilley, A., Davis, T. D., and Sankhla, N. 2000. Triazoles as plant growth regulators and stress protectants. Horticultural Reviews 24: 55-138.

11. Fowler, D. B., and Gusta, L. V. 1979. Selection for winter hardiness in wheat. I. Identification of genotypic variability. Crop Science 19: 769-772.

12. Gomathinayagam, M., Jaleel, C. A., Lakshmanan, G. M. A., and Panneerselvam, R. 2007. Changes in carbohydrate metabolism by triazole growth regulators in cassava (Manihot esculenta Crantz); effects on tuber production and quality. Comptes Rendus Biologies 330: 644-655.

13. Gopi, R., Jaleel, C. A., Sairam, R., Lakshmanan, G. M. A., Gomathinayagam, M., and Panneerselvam, R. 2007. Diferential efects of hexaconazole and paclobutrazol on biomass, electrolyte leakage, lipid peroxidation and antioxidant potential of Daucus carota L. Colloids and Surfaces B: Biointerfaces 60: 180-186.

14. Gusta, L. V., Fowler, D. B., and Tyler, N. J. 1982. Factors influencing hardening and survival in winter wheat. In: Plant cold hardiness and freezing stress - mechanisms and crop implications, Vol. 2, In. Li P.H. and Sakai A. (Eds.). Academic Press, New York, 23-40.

15. Gusta, L. V., O’Connor, B. J., Gao, Y. P., and Jana, S. 2001. A re-evaluation of controlled freeze-test and controlled environment hardening conditions to estimate the winter survival potential of winter wheats. Canadian Journal of Plant Science 81: 241-246.

16. Hana, B., and Bischofa, J. C., 2004. Direct cell injury associated with eutectic crystallization during freezing. Cryobiology 48: 8-21.

17. Hasegawa, P. M., Bressan, R. A., Zhu, J. K., and Bohnert, H. J. 2000. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51: 463-499.

18. Jami Al Ahmadi, M., and Kafi, M. 2008. Kochia (Kochia scoparia): To be or not to be? In: Crop and forage production using saline waters, In. Kafi M. and Khan M.A. (Eds.). NAM S&T Centre. Daya Publisher, New Delhi.

19. Kraus, T.E., and Fletcher, R. A. 1994. Paclobutrazol protects wheat seedlings from heat and paraquat injury. Is detoxification of active oxygen involved? Plant Cell Physiology 35: 45-52.

20. Kraus, T. E., Evans, R. C., Fletcher, R. A., and Pauls, K. P. 1995. Paclobutrazol enhances tolerance to increased levels of UV-B radiation in soybean (Glycine max) seedlings. Canadian Journal of Botany 73: 797-806.

21. Murry, G. A., Eser, D., Gusta, L. V., and Eteve, G. 1988. Winter hardiness in pea, lentil, faba bean and chickpea. In Summerfield, R.J. (Eds.), World Crops Cool Season Food Legumes. Kluwer Academic Publishers. The Netherlands. pp. 831-843.

22. Nabati, J. 2009. Effect of salinity on physiological characteristics and qualitative and quantitative traits of forage Kochia (Kochia scoparia). PhD Thesis. Ferdowsi University of Mashhad. (in Persian with English abstract).

23. Nayyar, H., Bains, T. S., and Kumar, S. 2005. Chilling stressed chickpea seedlings: effect of cold acclimation, calcium and abscisic acid on cryoprotective solutes and oxidative damage. Environmental and Experimental Botany 54: 275-285.

24. Nezami, A., Borzooei, A., Jahani, M., Azizi, M., and Sharif, A. 2007. Electrolyte leakage as an indicator of freezing injury in colza (Brassica napus L.). Iranian Journal of Field Crops Research 5: 167-175. (in Persian with English abstract).

25. Nezami, A., Nabati, J., Borzooei, A., Kamandi, A., Masomi, A., and Salehi, M. 2010. Evaluation of freezing tolerance in barley (Hordeum vulgar L.) cultivars at seedling stage under controlled conditions. Environmental Stresses in Crop Sciences 3: 9-22. (in Persian with English abstract).

26. Nezami, A., Solimani, M. R., Ziaee, M., Ghodsi, M., and Bannayan Aval, M. 2010. Evaluation of freezing tolerance of hexaploid triticale genotypes under controlled conditions. Notulae Scientia Biologicae 2: 114-120.

27. Paliyath, G., and Fletcher, R. A. 1995. Paclobutrazol treatment alters peroxidase and catalase activities in heat-stressed maize coleoptiles. Physiology and Molucular Biology of Plants 1: 171-178.

28. Rainha, N., Lima, E., Baptista, J., and Rodrigues, C. 2011. Antioxidant properties, total phenolic, total carotenoid and chlorophyll content of anatomical parts of Hypericum foliosum. Journal of Medicinal Plant Research 5: 1930-1940.

29. Rivero, R. M., Ruiz, J. M., Garcia, P. C., Lopez-Lefebre, L. R., Sanchez, E., and Romero, L. 2001. Resistance to cold and heat stress: Accumulation of phenolic compounds in tomato and watermelon plants. Plant Science 160: 315-321.

30. Sailerova, E., and Zwiazek, J. J. 1997. Early effect of triadimefon on water relations, sterol composition and plasma membrane ATPase activity in white spruce (Picea gluca) needles. Physiologia Plantarum 97: 747-753.

31. Sakai, A., and Larcher, W. 1987. Frost survival of plants: Responses and adaptation to freezing stress. Springer-Verlag, Berlin.

32. Senaratna, T., Mackay, C., McKersie, B., and Fletcher, R. 1988. Uniconazole-induced chilling tolerance in tomato and its relationship to antioxidant content. Journal of Plant Physiology 133: 56-61.

33. Shashikumar, K., and Nus, J. L. 1993. Cultivar and winter cover effects on Bermudagrass cold acclimation and crown moisture content. Crop Science 33: 813-817.

34. Upadhyaya, A., Davis, T., Walser, R., Galbraith, A., and Sankhla, N. 1989. Uniconazole-induced alleviation of low temperature damage in relation to antioxidant activity. Horticultural Science 24: 955-957.

35. Zhang, Q., Fry J., Rajashekar, C., and Bremer, D. 2009. Membrane polar lipid changes in Zoysiagrass rhizomes and their potential role in freezing tolerance. Journal of the American Society for Horticultural Science 134:322-328.