The Effect of Drought Stress and Plant Density on Biochemical and Physiological Characteristics of Two Garlic (Allium sativum L.) Ecotypes

Document Type : Research Article

Authors

• Sh Akbari
• M Kafi
• Sh Rezvan Beidokhti

Azad Islamic University Damghan branch

10.22067/gsc.v14i4.41406

Abstract

Introduction
Drought stress is the most important growth limiting factor for crop production. Sugar accumulation under drought stress strengthens and stabilizes cell membranes and maintains the water absorption and turgid property. Under stress conditions, proline will also maintain the turgor pressure and decreased the damages caused to plant membrane. Although proline concentrations may have undesirable effects on plant growth, because of deflecting photosynthetic resources to the processes that are not involved in plant growth. Chloroplasts and its pigments are also affected by drought stress. Density is one of the factors that has a significant impact on plant growth. Garlic is one of the edible plants which has generated considerable interest throughout human history because of its pharmaceutical properties. This study aimed to determine the effects of drought stress and plant density on some biochemical and physiological treats of two garlic ecotypes and determining the more resistant ecotype.
Materials and Methods
The study was carried out in 2011-2012 in a farm land at the south east of Semnan city. The experimental layout was a split-plot factorial with a randomized complete block design in three replications. The treatments were comprised of three factors: irrigation regimes (60%, 80% and 100% of estimated crop evapotranspiration (ETC)) that were assigned as the main plot and the factorial combination of 3 levels of planting density (30, 40 and 50 plants. m-2) and two ecotypes (Tabas and Toroud) made up the sub-plots. The water requirement was calculated based on FAO-56 crop water requirements instruction. FAO-56 Penman-Monteith equation was used to calculate evapotranspiration. To calculate the content of soluble sugar, proline and leaves pigment, the samples were collected in a random way from the youngest fully expended leaves one month before the final harvest. Relative water content was estimated by measuring dry weight, fresh weight and turgor weight. All calculations were performed with the help of the SAS and MSTATC soft wares. The statistical significances of mean values were assessed by analysing of variance and LSD tests at P

Keywords

• Chlorophyll
• Evapotranspiration
• Irrigation
• Proline
• Soluble sugars

20.1001.1.20081472.1395.14.4.9.2

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References

1. Abedi Baba Arabi, S., Movahhedi Dehnavi, M., Yadavi, A. R., and Adhami, E. 2011. Effects of ZN and K foliar application on physiological traits and yield of spring safflower under drought stress. Electronic Journal of Crop Production 4(1):75-95. (In Persian with English summary).

2. Allen, R. G., Periera, L.S, Raes, D., and Smith, M. 1998. Crop evapotranspiration guidelines for computing crop water requirements. FAO Irrigation and Drainage Paper 56. FAO, Rome.

3. Arvin, M.J., and Kazemi-Pour, N. 2003. Effects of salinity and drought stresses on growth and chemical and biochemical compositions of 4 Onion (Allium cepa) cultivars. Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil Science 5(4): 41-51. (In Persian).

4. Baghalian, K., Ziaee, A., Naghavi, M. R., and Naghdibady, H. A. 2004 Evaluation of garlic ecotypes Iranian culture allicin content and botanical characteristics. Medicinal Plant Journal 4(13): 50-59. (In Persian).

5. Bates, L. S., Waldran, R. P., and Teare, I. D. 1973. Rapid determination of free proline for water studies. Plant Soil 39: 205-208.

6. Ben Ahmed, C. H., Ben Rouina, B., Sensoy, S., Boukhris, M., and Ben Abdallah, F. 2009. Changes in gas exchange, proline accumulation and antioxidative enzyme activities in three olive cultivars under contrasting water availability regimes. Environmental and Experimental Botany 67(2): 345-352.

7. Bideshki, A., Arvin, M. J., and Maghsoudi, K. 2012. Effect of indole-3 butyric acid (IBA) foliar application on growth, bulb yield and allicin of garlic (Allium sativum L.) under water deficit stress in field. Iranian Journal of Medicinal and Aromatic Plants, 28(3): 567-577. (In Persian with English summary).

8. Bozin, B., Mimica-Dukic, N., Samojlik, I., Goran, A., and Igic, R. 2008. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chemistry 111: 925-929.

9. Buysee, J., and Mercks, R. 1993. An improved colorimetric method to quantify sugar content of plant tissue. Journal of Experimental Botany 44: 1627-1629.

10. Cha-um, S., Yooyongwech, S., and Supaibulwatana, K. 2010. Water deficit stress in the productive stage of four indica rice (Oryza sativa L.) genotypes. Pakistan Journal of Botany 42(5): 3387-3398.

11. Farshadfar, E., and Mohammadi, R. 2003. An evaluation of physiological indices of drought tolerance in Agropyron using multiple selection index. Iranian Journal of Agricultural Sciences 34(3): 635-646. (In Persian with English summary).

12. Hanson, B. R., May, D., Voss, R., Cantwell, M., and Rice, R. 2003. Response of garlic to irrigation water. Agricultural Water Management Journal 58: 29-43.

13. Hoekstra, F. A., and Buitink, J. 2001. Mechanisms of plant desiccation tolerance. Trends in Plant Science 8: 431-438.

14. Jones, M. M., Osmond, C. B., and Turner, N. C. 1980. Accumulation of solutes in leaves of sorghum and sunflower in response to water stress. Australian Journal of Plant Physiology 7: 193-205.

15. Kafi, M., Rezvan Beidokhti, Sh., and Sanjani, S. 2008. The effect of planting date and density on yield and morpho-physiological characteristics of Shallot (Allium altissimum Regel) in Mashhad. Journal of Horticultural Science 25(3): 310-319. (In Persian).

16. Kafi, M., Borzouey, A., Salehi, M., Kamandi, A., Masoumi, A., and Nabati, J. 2009. Environmental stress physiology of plants. Jahad Daneshgahi Mashhad press. (In Persian).

17. Kaiser, W. M. 1989. Effect of water deficit on photosynthetic capacity. Plant Physiology 71:142-149.

18. Kameli A., and LoÈsel, D. M. 1996. Growth and sugar accumulation in durum wheat plants under water stress. New Phytologist 132: 57- 62.

19. Keyvan, S. H. 2010. The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. Journal of Animal & Plant Sciences 8(3): 1051-1060.

20. Khajeh-pour, M. R. 2008. Principles and basics of agronomy. Jahad Daneshgahi Publications Centers of Industrial Branch of Isfahan. (In Persian).

21. Lichtenthaler, H. K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology 148: 350-382.

22. Lichtenthaler, H. K., and Buschmann C. 2001. Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Current Protocols in Food Analytical Chemistry 431-438.

23. Manivannan P., Jaleel C. A., Sankar B., Kishorekumar A., Somasundaram R., Lakshmanan G. M., and Panneerselvam, R. 2007. Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids Surf B Biointerfaces 59(2): 141-149.

24. Manoharan P. T., Shanmugaiah V., Balasubramanian N., Gomathinayagam S., Mahaveer P., Sharma M. P., and Muthuchelian, K. 2010. Influence of AM fungi on the growth and physiological status of Erythrina variegata Linn. Grown under different water stress conditions. European Journal of Soil Biology 46: 151-156.

25. Mirshekari, B., and Mobasher, M. 2010. Effect of sowing date, planting density and onion size on seed yield of Azarshahr Red onion variety in Tabriz. Journal of Agricultural Sciences 12(2): 398-404. (In Persian with English summary).

26. Mousavifar, B. E., Behdani M. A., Jami Al-Ahmadi, M., and Hosseini Bajd, M. S. 2011. Changes of chlorophyll index (SPAD), Relative water content, electrolyte leakage and seed yield in spring Safflower genotypes under irrigation termination. Iranian Journal of Field Crop Research 9(3): 525-534. (In Persian).

27. Naseri, Z., Abbassi, F., and Mahmoodzadeh, H. 2011. Effects of different water deficit levels and GA3 on the accumulation of proline and soluble and insoluble sugars in leaves of a new cultivar of barley (Hordeum vulgar L.). Journal of Plant Science Researches 6(2): 1-10. (In Persian with English summary).

28. Parameshwara, G., Paleg, I.G., Aspinall, D., and Jones, G. P. 1990. Proceedings of International Congress of Plant Physiology, New Delhi, India.

29. Sanchez, F.J., Manzanares, M., Andres, E. F., Temorio, J. l., Ayerbe, L., and De Andres, E .F. 1998. Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crop Research 59: 225-235.

30. Siddique, M. R. B., Hamid, A., and Islam, M. S. 2000. Drought stress effects on water relations of wheat. Botanical Bulletin of Academia Sinica 41: 35-39.

31. Veisipoor, A., Majidi, M. M., and Mirlohi, A. 2012. Traits relationship in Sainfoin (Onobrychis viciifolia) under normal and water stress conditions. Iranian Journal of Field Crop Science 42 (4): 745-756.

32. Xoconostle-Cazares, B., Ramirez-Ortega, F.A, Flores-Elenes, L., and Ruiz-Medrano, R. 2010. Drought tolerance in crop plants. American Journal of Plant Physiology 5(5): 241-256.

33. Zhu, Q. Y., Hackman, R. M., Ensunsa, J. L., Holt, R. R. and Keen, C. L. 2002. Antioxidative activities of oolong tea. Journal of Agriculture and Food Chemistry 50: 6929-6934.