Evaluation of Invasive Plant Centaurea balsamita Cold Acclimated in the Spring to Chilling and Freezing Str

Document Type : Research Article

Authors

• A Abbasian
• Gh Asadi
• R Ghorbani

Ferdowsi University of Mashhad

10.22067/gsc.v15i2.51308

Abstract

Introduction Invasive plants threaten the ecosystems of agriculture, forests and rangelands. In this regard, Centaurea balsamita is an annual plant of asteraceae family that invades the fallow and slope lands. This plant is reproduced by seed and distributed in Syria, Turkey, Iran, Afghanistan and Central Asia (Turkmenistan to Tian Shan). Given that, according to studies accomplished in the world, threats of invasive plants are widely known, and since most studies related to freezing and cold stress tolerance are conducted on crop plants and there is little information about invasive plants, and considering that the precise identification of problematic invasive species. This study was performed to evaluate invasive plant Centaurea balsamita cold acclimated in spring to chill and freezing stress.
Materials and Methods The experiments were conducted at Ferdowsi University of Mashhad, Faculty of Agriculture, in 2014 in a completely randomized design. In this experiment Centaurea balsamita in 2 to 4 and 4 to 6 leaf stage after the period of cold acclimation in the spring exposed to ten chilling and freezing temperatures (4, 2, 0, -2, -4,-6, -8, -10, -12 and -14 °C). In the experiment the pots were kept in the nursery from March 12, 2014 to April 19, 2014 and after cold acclimation period under these conditions, they were exposed to Chilling and freezing temperatures using thermogradient freezer. Freezer temperature was 8 °C at the beginning of the experiment and after placing the samples inside, the temperature decreased with the speed of 8 °C per hour. The cytoplasm membrane stability of Centaurea balsamita was evaluated using electrolyte leakage; then the lethal temperature of 50% of samples was determined based on leakage percentage (LT50el). Survival percentage was evaluated by counting the number of live plants in each pot. Then, at the end of the recovery period, the lethal temperature for 50% of survival percentage (LT50su) and reduction temperature for 50% of dry weight (RDMT50) were determined (three weeks after the application of freezing treatment).
Results and Discussion The lowest and highest percentage of electrolyte leakage in cold acclimated Centaurea balsamita, with spring conditions influenced by chilling and freezing temperatures were at 4 °C and -14 °C, respectively. Temperature reduction from -6 °C to -8 °C caused significant increase (P ≤ 0.05) in electrolyte leakage in Centaurea balsamita. In addition, the lowest and highest percentage of survival in cold acclimated Centaurea balsamita, with spring conditions influenced by chilling and freezing temperatures were observed at 4 °C and -14 °C, respectively. Temperature reduction from -6 °C to -8 °C caused significant decrease (P ≤ 0.05) in survival percentage in Centaurea balsamita. The highest and lowest dry weight in cold acclimated Centaurea balsamita, with spring conditions influenced by freezing temperatures, after the recovery period (21 days after application of freezing treatment) were observed at 4 °C and -14 °C, respectively. Temperature reduction to below -6 °C caused significant decrease (P ≤ 0.05) in biomass of Centaurea balsamita. According to the results of the present experiment, LT50el, LT50su and RDMT50 for acclimated Centaurea balsamita with spring conditions are -7.05 °C, -7.6 °C and -7.15 °C, respectively. Researchers reported that the plants with more tolerant to cold have less LT50el, LT50su and RDMT50.
Conclusions This study showed that in invasive plants such as Centaurea balsamita, using electrolyte leakage and survival tests can be relatively good methods in assessing and identifying invasive plants tolerant to cold, and help to identify invasive plant cold tolerance, predict their distribution and invasion. However, to ensure more about the cold tolerance of this invasive plant, it is recommended to cold acclimate this plant in areas with higher elevation and colder than Mashhad to achieve more accurate assessment of cold tolerance. Yet, due to low LT50su of this plant when distributed, migration this invasive plant to wheat, barley and rangelands is highly plausible.

Keywords

• LT50el
• LT50su
• RDMT50
• Recovery

20.1001.1.20081472.1396.15.2.12.8

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References

1. Anderson, J. A., Taliaferro, C. M., and Martin, D. L. 1993. Evaluating freeze tolerance of Bermuda grass in a controlled environment. Horticulture Sciences 28: 955.

2. Azizi, H., Nezami, A., Nassiri, M., and Khazaie, H. R. 2007. Evaluation of cold tolerance in Wheat (Triticum aestivum L.) cultivars under controlled conditions. Iranian Journal of Field Crops Research 5: 109-121. (in Persian with English abstract).

3. Baeka, K.H., and Skinner, D.Z. 2003. Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic Wheat lines. Plant Sciences 165: 1221-1227.

4. Bethany, A., Bradley. D. S., and Oppenheimer. M. 2010. Climate change increases risk of plant invasion in the Eastern United States. Biology Invasions 12: 1855-1872.

5. Bridger, G.M., Falk, D.E. Mckersie, B.D., and Smith, D.L. 1996. Crown freezing tolerance and field winter survival of winter cereals in eastern Canada. Crop Sciences 36: 150-157.

6. Cardona, C.A., Duncan, R.R., and Lindstorm, O. 1997. Low temperature tolerance assessment in paspalm. Crop Sciences 37: 1283-1291.

7. Carlson, M,. Heys, J., Shephard, M., and Snyder, J. 2005. Invasive Plants of Alaska. Conservation Districts Publication.

8. Chen, T.H., Gusta, L.V., and Fowler, D.B. 1983. Freezing injury and root development in winter cereals. Plant Physiology 73: 773-777.

9. Eugenia, M., Nunes, S., and Ray Smith, G. 2003. Electrolyte leakage assay capable of quantifying freezing resistance in Rose Clover. Crop Sciences 43: 1349-1357.

10. Fowler D.B., Gusta L.V., and Tyler N.J. 1981. Selection for winter hardiness in wheat. III. Screening methods. Crop Science 21: 896-901.

11. Griffith, M., and McIntyre, C.H. 1993. The interrelationship of growth and frost tolerance in winter rye. Plant Physiology 87: 335-344.

12. Hekneby, M., Antolin, M.C., and Sanchez-Diaz, M. 2006. Frost resistance and biochemical changes during cold acclimation in different annual legumes. Environmental and Experimental Botany 55: 305-314.

13. Izadi, E., Nezami, A., Abbassian, A., and Haydari, M. 2013 Investigation of wild oat fitness to freezing stress using of electrolyte leakage Index. environmental stress in crop Sciences 5 (1): 81-94. (in Persian with English abstract).

14. Levitt J. 1980. Chilling injury and resistance. In Kozlowsky, T.T. (eds.) Chilling, Freezing and High Temperature Stresses. Responses of Plants to Environmental Stresses. Academic Press, New York. 1: 23-64.

15. Moorby, J. 1981. Transport systems in plant. Longman Pub. New York.

16. Murata N., and Los D. A. 1997. Membrane fluidity and temperature perception. Plant Physiology 115: 875-879.

17. Nezami, A., Bagheri, A., Rahimian, H., Kafi, and Nasiri, M. 2006. Evaluation of freezing tolerance of Chickpea (Cicer arietinum L.) genotypes under controlled conditions. Journal of Sciences and Technology of Agriculture and Natural Resources 10 (4): 257-269. (in Persian with English abstract).

18. Nezami, A., Borzooei, A., Jahani, M., and Azizi, M. 2007. Evaluation of freezing tolerance in Canola (Brassica napus L.) cultivars under controlled conditions. Iranian Journal of Field Crops Research 5 (1): 167-175. (in Persian with English abstract).

19. Nezami, A., Hajmohammad nia Ghalibaf, K., and Kamandi, A. 2010. Evaluation of freezing tolerance in sugar beet (Beta vulgaris) cultivars under controlled condition. Environmental Stresses in Agricultural Sciences 3(2).

20. Nezami, A., Khazaei, H., Dashti, M., Mehrabadi, H.R., Eyshi Rezaee, E., and Ahmadi, M. 2013. Evaluation of Morpho-physiological indices in autumn sugar beet (Beta vulgaris L.) cultivars under freezing stress at seedling stage. Journal of Sugar beet 29 (1): 15-31.

21. Nezami, A., Soleimani, M. R., Ziaee, M., Ghodsi, M., and Bannayan, M. 2010. Evaluation of Freezing Tolerance of Hexaploid Triticale Genotypes under Controlled Conditions. Notulae Scientia Biologicae 114-120.

22. Paull, R.E. 1981. Temperature induced leakage from chilling–sensitive and chilling-resistant plant. Plant Physiology 68: 149-153.

23. Perras, M., and Sarhan, F. 1988. Synthesis of freezing tolerance proteins in leaves, crown and roots during cold acclimation of Wheat. Plant Physiology 89: 577-585.

24. Rashed, M.H., Nezami, A., Bagheri, A., Haj mohammadnia, K., and Bannayan, M. 2009. Evaluation of freezing tolerance of two fennel (Foeniculum vulgar L.) ecotypes under controlled conditions. Journal of Herbs, Spices and Medicinal Plants 15: 131-140.

25. Rechinger, Kh. 1979: Compositae-Cynareae. In: Rechinger KH, editor. Flora Iranica., Akademische Druckund Verlagsanstalt 139: 331-333.

26. Rife, C.L., and Zeinali, H. 2003. Cold tolerance in oilseed rape over varying acclimation durations. Crop Science 43: 96-100.

27. Shashikumar, K., and Nus, J.L. 1993. Cultivar and winter cover effects on bermudagrass cold acclimation and crown moisture content. Crop Sciences 33: 813-817.

28. Wagenitz, G. 2006. A Revision of Centaurea (Compositae-Cardueae) in The Flora of Iraq. Rostaniha 7 (2): 390-391.

29. Ya Li, H. D., and Gan Yao, Q. W. 2011. Impacts of Invasive Plants on Ecosystems in Natural Reserves in Jiangsu of China. Russian Journal of Ecology 42 (2): 133-137.