Improvement of Functional, Morphological, and Physiological Traits of Camelina (Camelina sativa) Using Iron Nanoparticle and Putrescine Sprying

Document Type : Research Article

Authors

1 M.Sc. graduted, Horticultural Science- Medicinal plants, Sana Institute of Higher Education, Sari, Iran

2 Biology Department, Sana Institute of Higher Education, Sari, Iran

3 Crop and Horticultural Science Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, AREEO, Sari, Iran

Abstract

Introduction
Applying innovative nanotechnology in agriculture is considered as one of the promising approaches to obtain significant increases of crop yield. Nanoparticles (NPs) are considered potential agents for agriculture as fertilizers and growth enhancers and using of nano-fertilizers has led to an increasing in the efficiency of nutrients, the correct management of fertilizer consumption, and a reduction of the frequency of fertilizer application. Stimulants are compounds that initiate signals for cells to increase or decrease the production of secondary metabolites and plant defense response. Stimulants such as Putrescine play a role in regulating various plant physiological processes. In this regard, considering the importance of using new technologies, including nanotechnology, in sustainable agriculture to increase the quantitative and qualitative performance of agricultural products, especially oil-medicinal plants, and the lack of sufficient information about the use of iron nanoparticles and polyamine putricine in Camelina plant nutrition, The effect of foliar spraying of these stimulants on functional, morphological and physiological traits of Camelina plant (Soheil veriety) was evaluated.
Materials and Methods
This research was conducted as factorial layout based on a randomized complete block design with three replications at the research fields of Baye-Kala Agricultural Research Station (BARS) at Neka city in 2021. The studied factors included iron nanoparticles in four concentrations (0, 20, 40, and 60 ppm) and polyamine putrescine in four concentrations (0, 0.5, 1, and 1.5 mM). Each experimental plot was prepared in 6 square meters area and the studied treatments were applied by foliar spraying at the beginning of the reproductive phase of the plant. Two weeks after applying the treatments, sampling was done to evaluate the different traits of the plant. After checking the normality of the data, they were analyzed with SAS (ver 9.1) software; obtained averages compared with using Duncan Test at the 5% probability level.
Results and Discussion
The results of variance analysis indicated the significance of the simple effect of iron nanoparticles and putrescine on all the studied traits at the 5% level, as well as the significance of the interaction of the two factors on all the studied traits except carotenoid and peroxidase in the reproductive stage at the 5% level. According to the results, the highest amount of carotenoid (0.142 and 0.141 mg.g-1, respectively) and peroxidase (4.96 and 4.38 mg.g-1, respectively) were observed in application of 60 ppm iron nanoparticles and 1.5 mM putrescine which had no statistically significant difference with the concentration of 40 ppm iron nanoparticles and 1 mM putrescine. The results indicated that the highest amount of flavonoid (40.72 mg.g-1), soluble sugar (139.27 mg.g-1), and plant height (115.75 cm) were observed in the treatment combination of 60 ppm iron and 1 mM putrescine, the highest percentage of oil (41.76) and protein (27.77) were observed in the treatment combination of 40 ppm iron and 1.5 mM putrescine and the highest amount of grain yield (210.27 g) and morphological components of yield were observed in the treatment combination of 40 ppm iron and 1 mM putrescine. The correlation result showed that there were the most positive and significant correlation values among the physiological traits and the yield trait had a positive and significant correlation with plant dry weight (0.44) and oil percentage (0.40).
Conclusion
The results have demonstrated that foliar application of 40 ppm iron nanoparticles and 1.5 mM putrescine significantly enhanced various growth characteristics, including photosynthetic pigment content, crude protein, oil content, as well as the physiological and morphological aspects of Camelina. Additionally, the application of iron nanoparticles and putrescine via a uniform supply of low-consumption nutrients has been shown to strengthen the plant's defense system, ultimately leading to improvements in the growth, development, and yield of the medicinal-oil Camelina plant.

Keywords

Main Subjects

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References

  1. Aghazadeh-Khalkhali, D., Mehrafarin, A., Abdossi, V., & Naghdi Badi, H. (2015). Mucilage and seed yield of psyllium (Plantago psyllium) in response to foliar application of nano-iron and potassium chelate fertilizer. Journal of Medicinal Plants, 14(56), 23-34. (In Persian). 20.1001.1.2717204.2015.14.56.4.1
  2. Akk, E., & Ilumae, E. (2005). Possibilities of growing Camelina sativa in ecological cultivation. Estonian Research Institute of Agriculture, Teaduse 13, 75501, Saku, Estonia.
  3. Alcázar, R., Marco, J. C., Cuevas, M., Patrón, A., Ferrando, P., Carrasco, A. F., Tiburcio, F., & Altabella, T. (2006). Involvement of polyamines in plant response to abiotic stress. Biotechnology Letters, 28, 1867-1876. https://doi.org/10.1007/s10529-006-9179-3
  4. Amaliotis, D., Velemis, D., Bladenopoulou, S., & Karapetsas, N. (2002). Leaf nutrient levels of strawberries (cv. Tudla) in relation to crop yield. Acta Horticulturae, 567, 447-450. https://doi.org/10.17660/actahortic.2002.567.93
  5. Amraee-Tabar, S., Ershdi, A., & Robati, T. (2016). The effect of putrescine and spermine on drought tolerance of almond and peach. Journal of Crops Improvement, 18, 203-218. (In Persian). https://doi.org/10.22059/jci.2016.56558
  6. Armin, M., Akbari, S., & Mashhadi, S. (2014). Effect of time and concentration of nano-Fe foliar application on yield components of wheat. International Journal of Biosciences, 4(9), 69-75. https://doi.org/10.12692/ijb/4.9.69-75
  7. Arnon, D. I. (1949). Copper enzymes in isolated chloroplast polyphenol oxide in betavulgaris. Plant Physiology, 24, 1-15.
  8. Askary, M., Amini, F., Talebi, M., & Shafiei Gavari, M. (2018). Effects of Fe-chelate and iron oxide nanoparticles on some of the physiological characteristics of alfalfa (Medicago sativa). Environmental Stresses in Crop Sciences, 11(2), 449-458, (In Persian). https://doi.org/10.22077/escs.2017.522.1104
  9. Azad, H., Fakheri, B., Mahdinejhad, N., & Parmoon, Gh. (2018). The study the efficacy of drought stress and foliar application of nano iron chelated on antioxidant enzymes activity and yield flower in plant in chamomile genotypes (Matricaria Chamomilla). Journal of Plant Process and Function, 7(26), 223-238. (In Persian). https://doi.org/20.1001.1.23222727.1397.7.26.3.2
  10. Bakhtiari, M., Moaveni, P., & Sani, B. (2015). The effect of iron nanoparticles spraying time and concentration on wheat. Biological Forum-An International Journal, 7(1), 679-683.
  11. Berti, M., Gesch, R., Eynck, C., Anderson, J., & Cermak, S. (2016). Camelina uses, genetics, genomics, production, and management. Industrial Crops Production, 94, 690-710. https://doi.org/10.1016/j.indcrop.2016.09.034
  12. Beygi, A., Oveisi, M., & Tarigh eslami, M. (2011). Investigating the effect of drought stress and foliar application of iron fertilizer in the flowering stage on the amount of seed yield, protein and seed oil in soybean plant. Conference on new achievements in the production of plants of oil origin. Bojnourd. Iran. (In Persian).
  13. Bradford, M. M. (1976). Rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248-254.
  14. Briat, J. F., Curie, C., & Gaymard, F. (2007). Iron utilization and metabolism in plants. Current Opinion in Plant Biology, 10, 276-282. https://doi.org/10.1016/j.pbi.2007.04.003
  15. Briat, J. F., Dubos, C., & Gaymard, F. (2015). Iron nutrition, biomass production, and plant product quality. Trends in Plant Science, 20(1), 33-40. https://doi.org/10.1016/j.tplants.2014.07.005
  16. Bromand-sivieri, M., Heydari, M., Gholami, A., & Ghorbani, H. (2021). Effects of biofertilizers and foliar application of iron oxide nanoparticle on grain yield and some physiological characteristics of black cumin (Nigella sativa). Iranian Journal of Field Crop Science, 51(4), 73-83. (In Persian). https://doi.org/10.22092/ijmapr.2020.125999.2542
  17. Chattopadayay, M. K., Tiwari, B. S., Chattopadhayay, G., Bose, A., Sengupta, D. N., & Ghosh, B. (2002). Protective role of exogenous polyamines on salinity-stressed rice (Oryza sativa) plants. Physiologia Plantarum, 116, 192-199. https://doi.org/10.1034/j.1399-3054.2002.1160208.x
  18. Chen, D., Shao, Q., Yin, L., Younis, A., & Zheng, B. (2019). Polyamine function in plants: Metabolism, regulation on development, and roles in abiotic stress responses. Frontiers in Plant Science, 9, 1945.
  19. Chinnamuthu, C. R., & Boopathi, M. P. (2009). Nanotechnology and agroecosystem. Plant Nutrition and Soil Science, 168, 558-573.
  20. Cohen, A. S., Popovic, R. B., & Zalik, S. (2004). Effects of polyamines on chlorophyll and protein content, photochemical activity and chloroplast ultrastructure of barley leaf discs during senescence. Plant Physiology, 64(5), 717-720. https://doi.org/10.1104/pp.64.5.717
  21. Couée, I., Hummel, I., Sulman, C., Gouesbet, G., & El-Amrani, A. (2004). Involvement of polyamines in root development. Plant Cell, Tissue and Organ Culture, 76, 1-10. https://doi.org/10.1023/a:1025895731017
  22. Das, C., Sengupta, T., Chattopadhyay, S., Setua, M., & Das, N. (2002). Involvement of kinetin and spermidine in controlling salinity stress in mulberry (Morus alba cv. S1). Acta Physiologiae Plantarum, 24, 53-57. https://doi.org/10.1007/s11738-002-0021-9
  23. Demirkiran, A. R. (2005). Determination of Fe, Cu and Zn Contents of Wheat and Corn grains from different growing site. Journal of Animal and Veterinary Advances, 8(8), 1563-1567. https://medwelljournals.com/abstract/?doi=javaa.2009.1563.1567
  24. Du, G., Li, M., Ma, F., & Liang, D. (2009). Antioxidant capacity and the relationship with polyphenol and vitamin C in Actinidia fruits. Food Chemistry, 113(2), 557-562. https://doi.org/10.1016/j.foodchem.2008.08.025
  25. El-Bassiouny, H. M., Mostafa, H. A., El-Khawas, S. A., Hassanein, R. A., Khalil, S. I., & Abd El- Monem, A. A. (2008). Physiological responses of wheat plant to foliar treatments with arginine or putrescine. Australian Journal of Basic and Applied Sciences, 2, 1390-1403.
  26. Gerami, M., Mohammadian, A., & Akbarpour, V. (2019). The Effect of Putrescine and Salicylic Acid on Physiological Characteristics and Antioxidant in Stevia Rebaudiana Under Salinity Stress. Journal of Crop Breeding, 11(29), 40-54. (In Persian). https://doi.org/10.29252/jcb.11.29.40
  27. Gill, S. S., & Tuteja, N. (2010). Polyamines and abiotic stress tolerance in plants. Plant Signal. Behavior, 5, 26-33. https://doi.org/10.4161/psb.5.1.10291
  28. Gupta, S., Sharma, M. L., Gupta, N. K., & Kumar, A. (2003). Productivity enhancement by putrescine in wheat (Triticum aestivum). Physiology and Molecular Biology of Plants, 9, 279-282.
  29. He, L., Nada, K., & Tachibana, S. (2002). Effects of spermidine pretreatment through the roots on growth and photosynthesis of chilled cucumber plants (Cucumis sativus). Journal of the Japanese Society for Horticultural Science, 71, 490-498. https://doi.org/10.2503/jjshs.71.490
  30. Hiyasmin Rose, L., Benzon, M., Rosnah, U., Rubenecia, V. U., Litra, J. R., & Sang Chul, L. (2015). Nano fertilizer affects on the growth, development, and chemical properties of rice. International Journal of Agronomy and Agricultural Research, 7(1), 105-117.
  31. Hussein, M. M., EL-Gereadly, N. H. M., & El-Desuki, M. (2006). Role of putrescine in resistance to salinity of pea plants (Pisum sativum). Journal of Applied Science Research, 2(9), 598-604.
  32. Ibrahim, F. M., & El Habbasha, S. F. (2015). Chemical composition, medicinal impacts and cultivation of camelina (Camelina sativa). International Journal of Pharm Tech Research, 8, 114-122.
  33. In, B. C., Motomura, S., Inamoto, K., Doi, M., & Mori, G. (2007). Multivariente analysis of realationbetween preharvest environmental factors, postharvest morphological and physiological factorsand vase life of cut Asomi Red Roses. Journal of the Japanese Society for Horticultural Science, 76, 66-72. https://doi.org/10.2503/jjshs.76.66
  34. Jabeen, N., & Ahmad, R. (2011). Effect of foliar-application boron and manganese on growth and biochemical activities in sunflower under saline conditions. Pakestanian Journal of Botany, 43, 1271-1282.
  35. Jafarpour, F., Bakhshi, D., Ghasem nejhad, M., & Hassan Sajedi, R. (2014). Effect of Putrescine on Postharvest Quality, and Phenolic Compounds and Antioxidant Capacity of Broccoli (Brassica oleracea cv. Italica) Florets. Journal of Horticultural Science, 28(3), 303-311. (In Persian).
  36. Jalil-Marandi, R. (2011). Physiology of environmental stresses and resistance mechanisms in garden plants. Two-volume course. First Edition. Urmia Academic Jihad Publications, Urmia (In Persian).
  37. Kenneth, H. (1990). Official Methods of Analysis (AOAC). 15th Association of Official Analytical Chemist, Washington DC.
  38. Lichtenthaler, H. (1987). Cholorophyllas and carotenoids: pigments of photosynthetic biomembranes. Methods of Enzymology, 148, 350-382.
  39. Liu, J. H., Wang, W., Wu, H., Gong, X., & Moriguchi, T. (2015). Polyamines function in stress tolerance: from synthesis to regulation. Frontiers in Plant Science, 6, 827. https://doi.org/10.3389/fpls.2015.00827
  40. Luck, H. (1974). In Methods in Enzymatic Analysis (ed. Bergmeyer, H.) p885. Journal of Academic press, New York.
  41. Mahros, K. M., Badawy, E. M., Mahgoub, M. H., Habib, A., & El-Sayed, I. (2011). Effect of putrescine and uniconazole treatments on flower characters and photosynthetic pigments of (Chrysanthemum indicum) Plant. Journal of American Science, 7(3), 399-408.
  42. Malakoti, M. J., & Tehrani, M. M. (1999). Effect of micronutriens on the Yield and Quality of Agricultural Products. Tarbiat Modarres University Press, Tehran. (In Persian).
  43. Marschner, H. (1995). Mineral nutrition of higher plants. Acadmic Press London pp: 313-323.
  44. Mazaherinia, M. A., Astaraei, R., Fotovat, A., & Monshi, A. (2010). Effect of Nano iron oxide particles and on Fe, Mn, Zn, Cu concentrations in wheat plants. World Applied Science, 7(1), 156-162.
  45. McCready, R. M., Guggolz, J., Silviera, V., & Owens, H. S. (1950). Determination of starch and amylose in vegetables. Analytical Chemistry, 22(9), 1156-1158.
  46. Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7, 405-410. https://doi.org/10.1016/s1360-1385(02)02312-9
  47. Mohammadi, M., Majnoun Hosseini, N., & Dashtaki, M. (2016). Effects of nano-ferric oxide and zinc sulfate on chlorophyll, anthocyanin, flavonoid and leaf mineral elements of peppermint (Mentha piperita) at Karaj climatic conditions. Iranian Journal of Medicinal and Aromatic Plants, 5(32), 770-784. (In Persian). https://doi.org/10.22092/ijmapr.2016.107994
  48. Mohamadipoor, R., Sedaghathoor, S., & MahboubKhomami, A. (2013). Effect of application of iron fertilizer in two methods foliar and soil application on growth characteristics of Spathyphyllum illusion. European Journal of Experimental Biology, 3(1), 232-240.
  49. Moradizadeh, M., Shamsi, V., & Morovvati, A. (2013). The effect of using iron nanochelate on the performance of Sirna variety sunflower in the Meybod region. The first national conference on sustainable development in arid and semiarid regions. Abarkouh. Yazd. (In Persian).
  50. Mosavi, S. R., Galavi, M., & Ahmadvand, G. (2007). Effect of zinc and manganese foliar application on yield, quality and enrichment on potato (Solanum tuberosum). Asian Journal of Plant Science, 6, 1256-1260. https://doi.org/10.3923/ajps.2007.1256.1260
  51. Mustafavi, S. H., Badi, H. N., Sekara, A., & Al, E. (2018). Polyamines and their possible mechanisms involved in plant physiological processes and elicitation of secondary metabolites. Acta Physiologiae Plantarum, 40, 102. https://doi.org/10.1007/s11738-018-2671-2
  52. Naderi, M. R., & Abedi, A. (2012). Application of nanotechnology in agriculture and refinement of environmental pollutants. Journal of Nanotechnology, 11(1), 18-26.
  53. Nassar, A. H., El-Tarabily, K. A., & Sivasithamparam, K. (2003). Growth promotion of bean (Phaseolus vulgaris) by a polyamineproducing isolate of Streptomyces griseoluteus. Plant Growth Regulation, 40, 97-106. https://doi.org/10.1023/a:1024233303526
  54. Peyvandi, M., Mirza, M., & Kamali Jamakani, Z. (2012). The Effect of Nano Fe Chelate and Fe Chelate on the Growth and Activity of some Satureja hortensis New Cellular and Molecular Biotechnology Journal, 2(5), 25-32. (In Persian). 20.1001.1.22285458.1390.2.5.3.2
  55. Peyvandi, M., Parande, H., & Mirza, M. (2011). Comparison of nano Fe chelate with Fe chelate effect on growth parameters and antioxidant enzymes activity of Ocimum Basilicum. New Cellular and Molecular Biotechnology Journal, 4, 89-99. (In Persian). 1001.1.22285458.1390.1.4.3.7
  56. Rady, M. M., El-Yazal, M. A. S., Taie, H. A. A., & Ahmad, S. M. A. (2016). Response of wheat growth and productivity to exogenous polyamines under lead stress. Journal of Crop Science and Biotechnology, 19, 363-371.
  57. Reis, R. S., Vale, E. M., Heringer, A. S., & Al, E. (2016). Putrescine induces somatic embryo development and proteomic changes in embryogenic callus of sugarcane. Journal of Proteomics, 130, 170-179. https://doi.org/10.1016/j.jprot.2015.09.029
  58. Sajedi, N., Ardakani, M., & Jafarzade, M. (2007). Investigating the effect of different levels of nitrogen, iron and zinc on the growth and absorption of nutrients and protein percentage by fodder corn variety Single Cross 704 in Markazi Province. 9th Iranian Congress of Agricultural Sciences and Plant Breeding. Tehran. Iran. (In Persian). https://civilica.com/doc/297703
  59. Seyed Sharifi, R., & Narimani, H. (2021). Effect of biofertilizers and putrescine on biomass and some physiological and biochemical traits of vetch (Vicia villosa Roth) under rainfed condition. Iranian Journal of Plant Biology, 13(3), 1-20. (In Persian). https://doi.org/10.22108/IJPB.2022.131436.1267
  60. Sozer, N., & Kokini, J. L. (2008). Nanotechnology and its application in the food sector (Review). Trends in Biotechnology, 27(2), 82-89. https://doi.org/10.1016/j.tibtech.2008.10.010
  61. Srivastava, N., Srivastava, M., Manikanta, A., Singh, P., Ramteke, P. W., Mishra, P. K., & Malhotra, B. D. (2017). Production and optimization of physicochemical parameters of cellulase using untreated orange waste by newly isolated Emericella variecolor NS3. Applied Biochemistry and Biotechnology, 183, 601-612. https://doi.org/10.1007/s12010-017-2561-x
  62. Taiz, L., & Zeiger, E. (2010). Plant Physiology. 5th Sinauer Associates Inc. Sunderland.
  63. Torabian, Sh., & Zahedi, M. (2013). Effects of Foliar Application of Common and Nano-sized of Iron Sulphate on the Growth of Sunflower Cultivars under Salinity. Iranian Journal of Field Crop Science, 44(1), 109-118. (In Persian). https://doi.org/10.22059/IJFCS.2013.30488
  64. Van Acker, S. A. B. E., Tromp, M. N. J. L., Haenen, G. R. M. M., Van Der Vijgh, W. J. F., & Bast, A. (1995). Flavonoids as scavengers of nitric oxide radical. Biochemical and Biophysical Research Communications, 214, 755-759. https://doi.org/10.1006/bbrc.1995.2350
  65. Verma, S., & Mishra, S. N. (2005). Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. Journal of Plant Physiology, 162, 669-677. https://doi.org/10.1016/j.jplph.2004.08.008
  66. Willekens, H., Chamnongpol, S., Davey, M., Schraudner, M., Angebartels, C., Van Montagu, M., & VanCamp, W. (1997). Catalase is a sink for H2O2 and is indispensable for stress defense in C3 plants. The EMBO Journal, 16, 4806-4816. https://doi.org/10.1093/emboj/16.16.4806
  67. Xie, Z., Jiag, D., Dai, T., Jing, Q., & Cao, W. (2004). Effects of exogenous ABA and cytokinin on leaf photosynthesis and grain protein accumulation in wheat ears cultured in vitro. Plant Growth Regulation, 44, 25-32. https://doi.org/10.1007/s10725-004-1880-4
  68. Yousefzadeh, S., Naghdi Badi, H. A., Sabaghaniya, N., & Jan Mommadi, M. (2016). The Effect of foliar application of nano-iron chelate on physiological and chemical traits of dragonhead (Dracocephalum moldavica). Journal of Medicinal Plants, 4(60), 152-160. (In Persian).
  69. Zahedi, H., & Alipour, A. (2018). Effect of spraying of iron and manganese nano chelated on yield and yield component of barley (Hordeum vulgare) under water deficit stress at different growth stages. Journal of Applied Crop Research, 11(4), 847-861. (In Persian). https://doi.org/10.22077/escs.2018.890.1176
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Iranian Journal of Field Crops Research
Volume 21, Issue 4 - Serial Number 72
January 2024
Pages 403-418

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  • Receive Date: 26 March 2023
  • Revise Date: 30 July 2023
  • Accept Date: 05 August 2023
  • First Publish Date: 05 August 2023

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