Investigating the Changes of Some Agronomic and Biochemical Characteristics of Thymus vulgaris L. with Application of Mycorrhizal Species and Foliar Spraying Humic Acid

Document Type : Research Article

Authors

1 Department of Agriculture, Payam Noor University, Tehran, Iran

2 Department of Agriculture and Environment, Payam Noor University, Tehran, Iran

Abstract

Introduction
Garden thyme (Thymus vulgaris L.), is a small perennial plant native to the Mediterranean region that is now found worldwide. It is commonly used as a culinary herb and for medicinal purposes as well (Silva et al., 2021). Thymol is one of the main and active chemical compounds of thyme. It has been shown to have antibacterial, antifungal and antiviral properties and is often used as a natural preservative in food and cosmetic products (Hammoudi et al., 2022). Golubkina et al. (2020) reported that the use of mycorrhizal fungi as a technology that is compatible with the environment and by increasing plant access to nutrients and increasing resistance to stresses as well as improving water absorption, can lead to improved plant growth. Today, the use of organic compounds such as humic acid can affect many morphological, physiological and biochemical processes of plants and lead to improvement in plant growth (Mousavi et al., 2022).
Materials and Methods
In order to investigate the effects of foliar application of humic acid fertilization and mycorrhizal fungi symbiosis on Thymus vulgaris L., a factorial layout has been applied, based on a randomized complete block design with three replications at the field of the research farm of Islamic Azad University, Takestan Branch during 2020-2021 and 2021-2022 growing seasons. Experimental factors included three species of mycorrhiza fungi (control, Glomus mosseae, G.etunicatum and G.intradices) and humic acid fertilizer levels included (control, 150 and 300 mg.L-1). Important traits such as plant height, herbal fresh weight, essential oil percentage, and yield, flavonoid content, root colonization percentage, thymol and carvacrol percentage were measured and analyzed.
Results and Discussion
The results showed that the effects of the treatments were significant at the 1% level for all investigated traits. For the characteristics of essential oil percentage and essential oil yield, the interaction effect was also significant at 1%, with an observed increase of over 200%. The application of 300 mg/liter humic acid resulted in a 14% increase in root colonization and a 16% increase in flavonoid percentage. Similarly, the application of mycorrhiza increased these traits by 23% and 30%, respectively. Thymol and carvacrol were the most abundant essential oil compounds. It can be concluded that the quality of thyme essential oil improved under the treatments. Among the mycorrhizae used in this research, G. mosseae mycorrhizae were more effective than the other two types. The results suggest that the application of mycorrhiza has a more significant impact on improving the examined traits compared to humic acid.
Conclusion
Based on the results of this study, application of mycorrhiza due to creating a symbiotic relationship with the roots of the garden thyme plant and humic acid spraying could increase the quantity and quality of the produced product. mycorrhiza was better than the use of humic acid in most treatments.

Keywords

Main Subjects

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References

  1. Ahmadzadeh, M., Sedaghati, E., Sabri-Riseh, R., Rahimi, A., Hatami, N., & Mohammadi Mirik, A. A. (2021). Evaluation of root mycorrhizal colonization and uptake of some microelements by Zea mays root treated with some organic compounds and microbial inoculation. Agricultural Engineering, 44(2), 141-158. https://doi.org/10.22055/AGEN.2021.36868.1602
  2. Akbari, I., & Gholami, A. (2016). Evaluation of Mycorrhizal Fungi, Vermicompost and Humic Acid on Essence Yield and Root Colonization of Fennel. Iranian Journal of Field Crops Research, 13(4), 840-853. (in Persian with English abstract). https://doi.org/10.22067/GSC.V13I4.42567
  3. Akbari, T., Rostami, M., Ghabooli, M., & Movahedi, Z. (2021). Effect of piriformospora indica on reducing the negative effects of salinity stress in lemon balm (Melissa officinalis). Crop Science Research in Arid Regions, 2(2), 219-229. (in Persian with English abstract). https://doi.org/10.22034/CSRAR.2021.265529.1077
  4. Alavi Asl, S. A., Majidian, M., Modares Sanavy, S. A. M., & Esfahani, M. (2023). Effect of Wood Vinegar and Humic Acid on Morphological and Biochemical Traits, Antioxidant Enzymes, and Essential Oil of Peppermint (Mentha piperita) under Water Deficit Stress Conditions. Iranian Journal of Field Crop Science, 54(3), 163-176. (in Persian with English abstract). https://doi.org/10.22059/ijfcs.2023.356625.654990
  5. Alizadeh Ahmadabadi, A., Khorasaninejad, S., & Hemti, K. (2016). Effect of Drought Stress and Humic Acid on Content Phenol, Flavonoid and Antioxidant of Purple coneflower (Echinacea purpurea). The first national conference of medicinal, aromatic and spicy plants. Gonbad Kavus University. 2016.
  6. Alizadeh, A., Najafi, F., Hadian, J., & Salehi, P. (2018). Effect of different levels of humic-acid and vermicompost extract on growth, yield, morphological and phytochemical properties of Satureja khuzistanica Journal of Agroecology, 10(1), 69-80. (in Persian with English abstract). https://doi.org/10.22067/jag.v10i1.47161
  7. Almeida, D. J., Alberton, O., Otênio, J. K., & Carrenho, R., (2020). Growth of chamomile (Matricaria chamomilla ) and production of essential oil stimulated by arbuscular mycorrhizal symbiosis. Rhizosphere, 15, 100208. https://doi.org/10.1016/j.rhisph.2020.100208
  8. Al-Saif, A. M., Sas-Paszt, L., Awad, R. M., & Mosa, W. F. A. (2023). Apricot (Prunus armeniaca) Performance under Foliar Application of Humic Acid, Brassinosteroids, and Seaweed Extract. Horticulturae, 9(4), 519. https://doi.org/10.3390/horticulturae9040519
  9. Amani Machiani, M., Javanmard, A., Ostadi, A., Aghaee, A., & Rasouli, F. (2021b). Effects of arbuscular mycorrhizal fungi and harvesting time on the macro- and micronutrients and antioxidant enzymes activity of thyme (Thymus Vulgaris) in the different irrigation levels. Plant Process and Function, 10(44), 235-250. (in Persian with English abstract).
  10. Amani Machiani, M., Javanmard, A., Ostadi, A., Morshedlou, M., & Chabokpour, J. (2021a). Effects of harvest time and mycorrhiza fungus application on quantitative and qualitative yield of thyme (Thymus vulgaris) essential oil at different irrigation levels. Iranian Journal of Medicinal and Aromatic Plants Research, 36(6), 1022-1037. (in Persian with English abstract). https://doi.org/10.22092/ijmapr.2021.351323.2835
  11. Arjmand Alavi, M., Hatamzadeh, A., & Ehteshami, S. M. (2014). Effect of bulb inoculation with four species mycorrhizal fungi on quantitative and qualitative yield of two lily species. Iranian Journal of Seed Science and Research, 1(2), 57-65. (in Persian with English abstract).
  12. Auge, R. M., Stodola, A. J. W., Tims, J. E., & Saxton, A. M. (2001). Moisture retention properties of a mycorrhizal soil. Plant and Soil, 230, 87-97.
  13. Azimi, R., Jangju, M., & Asghari, H. (2014). The effect of mycorrhiza inoculation on the initial establishment and morphological characteristics of the thyme medicinal plant in natural conditions. Iranian Journal of Field Crops Research, 11(4), 666-676. (in Persian). https://doi.org/10.22067/GSC.V11I4.32903
  14. Azizi, M., & Safaii, Z. (2017). The Effect of Foliar Application of Humic Acid and Nano Fertilizer (Pharmks®) on Morphological Traits, Yield, Essential Oil Content and Yield of Black Cumin (Nigella sativa). Journal of Horticultural Science, 30(4), 671-680. (in Persian). https://doi.org/10.22067/jhorts4.v0i0.41136
  15. Baum, C., El-Tohamy, W., & Gruda, N. (2015). Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi: a review. Scientia Horticulturae, 187, 131-141. https://doi.org/10.1016/j.scienta.2015.03.002
  16. Begum, N., Qin, C., Ahanger, M. A., Raza, S., Khan, M. I., Ashraf, M., Ahmed, N., & Zhang, L. (2019). Role of arbuscular mycorrhizal fungi in plant growth regulation: implications in abiotic stress tolerance. Frontiers in Plant Science, 10, 1-15. https://doi.org/3389/fpls.2019.01068
  17. Boutasknit, A., Baslam, M., Ait-El-mokhtar, M., Anli, M., Ben-Laouane, R., Douira, A., Modafar, C. E., Mitsui, T., Wahbi, S., & Meddich, A. (2020). Arbuscular mycorrhizal fungi mediate drought tolerance and recovery in two contrasting carob (Ceratonia siliqua ) ecotypes by regulating stomatal, water relations, and (in) organic adjustments. Plants, 9, 1-19. https://doi.org/10.3390/plants9010080
  18. Canellas, L. P., Olivares, F. L., Canellas, N. O., Mazzei, P., & Piccolo, A. (2019). Humic acids increase the maize seedlings exudation yield. Chemical and Biological Technologies in Agriculture, 6(1), 1-14. https://doi.org/10.1186/s40538-018-0139-7
  19. Csongor, B., Viktória, L. B., Erika, K., Béla, K., Dávid, U. N., Péter, S., Giuseppe, M., Luigi, M., Judit, K., Dóra, P., & Györgyi, H. (2023). Flowering phenophases influence the antibacterial and anti-biofilm effects of Thymus vulgaris essential oil. BMC Complementary. Medicine and Therapies, 23, 168. https://doi.org/10.1186/s12906-023-03966-1
  20. Dordas, C. A., & Sioulas, C. (2008). Safflower yield, chlorophyll content, photosynthesis, and water use efficiency response to nitrogen fertilization under rainfed conditions. Industrial Crops and Products, 27(1), 75-85.
  21. Doskocil, L., Szewieczková, J. B., Enev, V., Kalina, L., & Wasserbauer, J. (2018). Spectral characterization and comparison of humic acids isolated from some European lignites. Fuel, 213, 123-132. https://doi.org/10.1016/j.fuel.2017.10.114
  22. Golubkina, N., Logvinenko, L., Novitsky, M., Zamana, S., Sokolov, S., Molchanova, A., Shevchuk, O., Sekara, A., Tallarita, A., & Caruso, G. (2020). Yield, essential oil and quality performances of Artemisia dracunculus, Hyssopus officinalis and Lavandula angustifolia as affected by arbuscular mycorrhizal fungi under organic management. Plants, 9, 1-16. https://doi.org/10.3390/plants9030375
  23. Gorgini Shabankareh, H., Khorasani nejad, S., Sadeghi, M., & Tabasi, A. (2018). Effect of irrigation cycle and humic acid on morphophysiological and biochemical properties of thyme medicinal plant. Journal of Plant Environmental Physiology, 13(51), 67-82. (in Persian).
  24. Haghir Ebrahimabadi, A., Hatami, M., Karimzadeh Asl, Kh., & Ghorbanpour, M. (2018). Effect of Mycorrhizal Fungi and Biophosphor Fertilizer on Growth Features, Yield and Yield Components, and Essntial Oil Constituents in Cuminum cyminum Journal of Medicinal Plants, 17(2), 74-91. (in Persian with English abstract).
  25. Hammoudi Halat, D., Krayem, M., Khaled, S., & Younes, S. (2022). A focused insight into thyme: Biological, chemical, and therapeutic properties of an indigenous Mediterranean herb. Nutrients, 14(10), 2104. https://doi.org/10.3390/nu14102104
  26. Kapoor, R., Chaudhary, V., & Bhatnagar, A. (2007). Effects of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua Mycorrhiza, 17(7), 581-587. https://doi.org/10.1007/s00572-007-0135-4
  27. Kormanik, P. P., & McGraw, A. C. (1982). Quantification of vesicular-arbuscular mycorrhizae in plant roots. In Methods and Principles of Mycorrhizal Research (N. C. Schenck, Ed.). pp. 37-45. American Phytopathological Society, st Paul
  28. Lotfollahi, A., Bolandnazar, S., Aliasgharzad, N., Khoshru, B., & Siami. A. (2020). Effects of Inoculation with Arbuscular Mycorrhiza and Mycorrhiza-Like Fungi on Growth and Phosphorus Uptake of Coriander. Scientific Research Journal of Agricultural Knowledge and Sustainable Production, 31(1). 87-101. (in Persian with English abstract). https://doi.org/10.22034/SAPS.2021.12791
  29. Mafakheri, S., & Asghari, B. (2018). Effect of Seaweed Extract, Humic Acid and Chemical Fertilizers on Morphological, Physiological and Biochemical Characteristics of Trigonella foenum-graecum Journal of Agricultural Science and Technology, 20, 1505-1516.
  30. Mohammadi Nia Samakoush, A., Moradi, H., & Akbarpour, V. (2022). Investigating the effect of humic acid treatment on antioxidant capacity, phenol and photosynthetic pigments of the medicinal plant Artemisia annua under salt stress. Scientific Research Journal of Plant Ecophysiology, 13(46), 2-14. (in Persian).
  31. Monda, H., McKenna, A. M., Fountain, R., & Lamar, R. T. (2021). Bioactivity of Humic Acids Extracted From Shale Ore: Molecular Characterization and Structure-Activity Relationship with Tomato Plant Yield under Nutritional Stress. Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.660224
  32. Morshedloo, M. R., Maggi, F., Neko, H. T., & Aghdam, M. S. (2018). Sumac (Rhus coriaria) fruit: essential oil variability in Iranian populations. Industrial Crops and Products, 111, 1-7. https://doi.org/10.1016/j.indcrop.2017.10.002
  33. Mortazaei, S., Rafieian, M., Ansary Samani, R., & Shahinfard, N. (2012). Comparison of Phenolic Compounds Concentrations and Antioxidant Activity of Eight Medicinal Plants. Journal of Rafsanjan University of Medical Sciences, 12(7), 519-530.
  34. Mousavi, S. A. H., Barzegar, T., Nekounam, F., Ghahremani, Z., & Khani, A. (2022). The effect of humic acid on physiological characteristics, antioxidant activity and yield of Cape gooseberry (Physalis peruviana) under deficit irrigation. Plant Process and Function, 12(54), 171-185. (in Persian).
  35. Naji, Z., Babaakbari Sari, M., Vaezi, A., & Ahmadi, Sh. (2020). The Effect of Different Levels of Polyacrylic and Humic Acid on Aggregates Stability and Soil Moisture Content of Saline and Sodic Soils. Iran Water and Soil Research, 52(1), 15-24. (in Persian with English abstract). https://doi.org/10.22059/ijswr.2020.286599.668281
  36. Nardi, S., Pizzeghello, D., Muscolo, A., & Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, 34, 1527-1536.
  37. Nasiri Dehsorkhi, A., Makarian, H., Varnaseri Ghandali, V., & Salari, N. (2018). Investigation of effect of humic acid and vermicompost application on yield and yield components of cumin (Cuminum cyminum). Applied Research in Field Crops, 31(1), 93-113. (in Persian with English abstract). https://doi.org/10.22092/AJ.2018.121407.1277
  38. Saadi Moghaddam, S., Javanmard, A., Morshedloo, M. R., & Nouraein, M. (2020). Effect of integrated application of biological and chemical fertilizers on the quality ‎and quantity traits of dragon's head (Lallemantia iberica) under rainfed condition‎. Iranian Journal of Horticultural Science, 51(2), 459-477. (in Persian with English abstract). https://doi.org/10.22059/ijhs.2019.273123.1578
  39. Sabbagh, S., Sarafraz, M., Taheri, M., & Bolok, H. (2020). Effect of glomus intraradices fungus on enzymatic activities and growth condition of seven wheat genotypes. Crop Science Research in Arid Regions, 2(1), 43-53. (in Persian with English abstract). https://doi.org/10.22034/csrar.2020.119122
  40. Sabouri, F., Sirousmehr, A., & Gorgini Shabankareh, H. (2018). Effect of irrigation regimes and application of humic acid on some morphological and physiological characteristics of Savory (Satureja hortensis). Iranian Journal of Plant Biology, 9(34), 13-24. (in Persian with English abstract).
  41. Sanchez, F. J., Manzanares, M., de Andres, E. F., Tenorio, J. L., & Ayerbe, L. (1998). Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research, 59(3), 225-235. https://doi.org/10.1016/S0378-4290(98)00125-7
  42. Sangwan, N. S., Farooqi, A. H. A., Shabih, F., & Sangwan, R. S. (2001). Regulation of essential oil production in plants. Plant Growth Regulation, 34, 3-21.
  43. Siddiqui, M. H., Oad, F. C., & Jmaro, M. G. H. (2006). Emergence and nitrogen use efficiency of maize under different tillage operation and fertility levels. Asian Journal of Plant Sciences, 5(3), 508-510. https://doi.org/10.3923/ajps.2006.508.510
  44. Silva, A. S., Tewari, D., Sureda, A., Suntar, I., Belwal, T., Battino, M., Nabavi, S. M., & Nabavi, S. F. (2021). The evidence of health benefits and food applications of Thymus vulgaris Trends in Food Science & Technology. 117, 218-227. https://doi.org/10.1016/j.tifs.2021.11.010
  45. Stefanaki, A., & Andel, T. van. (2021). Mediterranean aromatic herbs and their culinary use. Aromatic herbs in food bioactive compounds, processing, and applications. 93-121. Publisher: Elsevier. Aromatic Herbs in Food. https://doi.org/10.1016/B978-0-12-822716-9.00003-2
  46. Tabrizi, L., Mohammadi, S., Delshad, M., & Moteshare Zadeh, B. (2015). The Effect of Arbuscular Mycorrhizal Fungi on Growth and Yield of Rosemary (Rosmarinus officinalis) under Lead and Cadmium Stress. Environmental Science Quarterly, 13(2), 37-48.
  47. Taheri Asghari, M. (2021). Effect of seed inoculation with Mycorrhiza fungi and leaf application of amino acid on some qualitative and herbaceous properties of pot marigold. Iranian Journal of Seed Science and Research, 9(3), 57-71. (in Persian with English abstract). https://doi.org/10.22124/jms.2022.6164
  48. Tarraf, W., Ruta, C., Tagarelli, A., De Cillis, F., & De Mastro, G. (2017). Influence of arbuscular mycorrhizae on plant growth, essential oil production and phosphorus uptake of Salvia officinalis L. Indian Journal Crop Products Process, 102, 144-153. (in Persian with English abstract). https://doi.org/10.1016/j.indcrop.2017.03.010
  49. Weisany, W., Raei, Y., Salmasi, S. Z., Sohrabi, Y., & Ghassemi-Golezani, K. (2016). Arbuscular mycorrhizal fungi induced changes in rhizosphere, essential oil and mineral nutrients uptake in dill/common bean intercropping system. Annals of Applied Biology, 169(3), 384-397. https://doi.org/10.1111/aab.12309
  50. Yadegari, M. (2022). Effects of NPK, botamisol, and humic acid on morphophysiological traits and essential oil of three Satureja species under drought stress. Iranian Journal of Medicinal and Aromatic Plants Research, 38(1), 61-80. https://doi.org/10.22092/ijmapr.2022.356264.3073
  51. Yadegari, M. (2022a). Effects of NPK complete fertilizer, botamisol, and humic acid on morphophysiological characteristics and essential oil in three Thymus species under drought stress conditions. Iranian Journal of Medicinal and Aromatic Plants Research, 38(2), 301-321. https://doi.org/10.22092/IJMAPR.2022.357303.3119
  52. Yazdan panah gohari, A., Ghanbari jahromi, M., & Zarrin nia, V. (2021). Effect of Some Mycorrhizal Fungi Species on Quantitative and Qualitative Properties of Two Landraces of Chicory in Greenhouse Conditions. Journal Crop Improv, 24(2), 527-544. https://doi.org/10.22059/jci.2021.314730.2484
  53. Yousefi, M., Enteshari, Sh., & Saadatmand, M. (2014). Effects of silica treatment on some morphological, anatomical and physiological characteristics of Iranian borage (Echium amoenum Fisch & C.A. Mey). Soil Culture Research Center, 5(2), 83-94. (in Persian).
  54. Zhang, F., Zou, Y. N., & Wu, Q. S. (2018). Quantitative estimation of water uptake by mycorrhizal extraradical hyphae in citrus under drought stress. Scientia Horticulturae, 229, 132-136. https://doi.org/10.1016/j.scienta.2017.10.038
  55. Zhishen, J., Mencheng, T., & Jianming, W. (1999). The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
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Iranian Journal of Field Crops Research
Volume 22, Issue 3 - Serial Number 75
October 2024
Pages 261-276

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  • Receive Date: 03 December 2023
  • Revise Date: 02 May 2024
  • Accept Date: 07 May 2024
  • First Publish Date: 31 July 2024

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