Water Stress Alleviation in Triticale Using of Bio-fertilizer and Intercropping Systems in an Arid Area of Southern Iran

Document Type : Research Article

Authors

• I Rashidipour ¹
• V Barati ²

¹ MSc Student, Agro-Ecology Department, Faculty of Agriculture and Natural Resources of Darab, Shiraz University, Shiraz, Iran

² Agro-Ecology Department, Faculty of Agriculture and Natural Resources of Darab, Shiraz University, Shiraz, Iran

10.22067/jcesc.2023.82040.1239

Abstract

Introduction
South of Iran has been located in the dry belt and desert strip thus water stress has always been one of the serious problems in its agriculture (Buzarjomehri et al., 2020). Intercropping is the cultivation of two or more plant species in a specific land and growing season, which is important in agricultural systems with limited resources and low input (Brooker et al., 2015). Due to the differences in the rooting depth, lateral expansion, and root density of cereals and legumes, they have been the best candidates for intercropping traditionally for limited soil water and nutrient availability environments (Babalola, 1980; Haynes, 1980). Application of bio-fertilizers (PGPR bacteria) that have nitrogen (N) fixation and phosphorus (P) solubilizing activity (Azospirillum brasilense and Pseudomonas fluorescence, respectively) is a promising approach for obtaining N, P, and water-restricted areas (Tien et al, 1979; Barea, 2015). Organic manures enhance soil water holding capacity and serve as excellent slow-release sources of nitrogen (N) and phosphorus (P) in the soil (Risse et al., 2006). This study aimed to investigate the effect of different fertilizer systems (chemical, integrated, and bio-organic) on triticale grain yield and its components in sole and intercropped triticale in triticale/chickpea system under late season water stress in a hot and dry area of southern Iran (Fars province - Darab).
Materials and Methods
To evaluate the yield and yield components of triticale (× Triticosecale Wittmack) in monoculture and intercropping with chickpea (Cicer arietinum L.) under late season water stress conditions, a split-factorial experiment in a randomized complete block design with three replications was implemented at the research field of the College of Agriculture and Natural Resources of Darab - Shiraz University in 2019-2020 growing season. Treatments were two levels of irrigation (Ir): [1- normal (IR_N): irrigation based on the plant water requirement up to the physiological maturity stage (ZGS92) and 2- water stress (WS): irrigation based on the plant water requirement up to the milking stage] as the main plots and three fertilizer sources (F_S) [1- chemical: ( 50 kg P ha^-1 + 150 kg N ha^-1), 2- integrated: (25 kg P ha^-1 + 75 kg N ha^-1 + 20 tons sheep manure ha^-1 + inoculation with Pseudomonas fluorescens and Azospirillum brasilense) 3- bio-organic: 40 tons sheep manure ha^-1 + inoculation with Pseudomonas fluorescens and Azospirillum brasilense] and two cropping systems (Cs) [1- monoculture of triticale, 2- intercropped triticale with chickpea (1:1)] as the sub-plots. Triticale grain yield and its components were measured and the harvest index (%) was calculated. Data were analyzed using SAS 9.1 software and the means were separated by the least significant difference (LSD) test at 5% probability level.
Results and Discussion
The results demonstrated that water stress reduced the grain yield and its components in triticale, with the extent of reduction varying among different treatments. In the Ir × Cs interaction, the Ws treatment reduced the triticale grain yield as compared to IR_N by 38% and 55 % in the intercropped and sole-triticale, respectively. The Ir × Fs interaction showed that the grain yield decreased by water stress as a function of the fertilizer system (60.4%, 43.7%, and 30.7% reduction in the chemical, integrated, and bio-organic treatments, respectively). Evaluation of the results related to the triticale yield components (grain weight, biological yield, and harvest index) also showed that the Ws treatment reduced these traits. However, the least reduction occurred in bio-organic fertilizer as compared with the other fertilizer systems (significant Ir × Fs interaction). Furthermore, the Ir × Cs interaction showed that the Ws treatment reduced triticale grain weight and its biological yield as compared to IR_N in both cropping systems with different manners. The least reduction of their traits by Ws treatment was showed in intercropped triticale as compared to its sole cropping.
Conclusion
Based on the results of this study, intercropped triticale with chickpea and using of bio-organic fertilizer were recommended for hot and dry areas of southern Iran where water stress may occur at the end of the growing seasons. Using these strategies leads to water stress alleviation and therefore less reduction of triticale grain yield in these environments.

Keywords

• Azospirillum
• Pseudomonas
• Grain yield
• Integrated fertilizer

20.1001.1.20081472.1402.21.4.3.5

Main Subjects

• Mixed cultivation

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©2024 The author(s). This article is licensed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

References

Abdolahi, A., Dabbagh Mohammadinasab, A., & Nasrolahzadeh, S. (2015) Investigation of yield and some traits of wheat and weeds in intercropping with chickpea under nitrogen management in rainfed condition. Cereal Research, 5 (3), 247-259. (In Persian with English abstract). https://dorl.net/dor/20.1001.1.22520163.1394.5.3.4.9

Ahmadvand, G., & Hajinia, S. (2016). Ecological aspects study of replacement intercropping patterns of Soybean (Glycine max L.) and Millet (Panicum miliaceum L.). Journal of Agroecology, 7(4), 485-498.

Asadi, Gh. A., and Khorramdel, S. (2014). Ratio effects of barley intercropped with hairy vetch on plant nitrogen content, population and diversity of weeds and yield. Gorgan university agriculture science and natural resources, 7(1), 131-156. (In Persian with English abstract). https://dorl.net/dor/20.1001.1.2008739.1393.7.1.8.1

Babalola, O. (1980). Water relations of three cowpea cultivars (Vigna unguiculata L.). Plant and Soil, 56(1): 59-69. https://doi.org/10.1007/bf02197953

Bahari Saravi, S. H., & Pirdashti, H. A. (2013). Evaluation of application of plant growth promoting bacteria (PGPR) and phosphate solvent (PSM) on yield and yield components of wheat (cultivar N80) at different levels of nitrogen and phosphorus in greenhouse conditions. Iranian Journal of Field Crops Research, 10(4), 681-689. (In Persian).

Barati, S., Basiri, M., Vahabi, M. R., & Mosadeghi, M. R. (2020). Effects of plant density and drought stress on Alfalfa Leaf Water potential (Medicago sativa L.) and Flesh Grass (Bromus tomentellus Boiss) and Soil weight moisture in sole cropping and intercropping. Range Land, 13(1), 65-75. http://dorl.net/dor/20.1001.1.20080891.1398.13.1.6.7

Barati, V., & Bijanzadeh, E. (2020). Grain Yield and its Components of triticale as affected by Silicon Foliar Application, Nitrogen Fertilizer and Water Stress in Reproductive Phase. Iranian Journal of Field Crops Research, 18(4), 435-449. (In Persian with English abstract). https://doi.org/10.22067/jcesc.2020.88386 

Barati, V., & Ghadiri, H. (2016). Effects of Drought Stress and Nitrogen Fertilizer on Yield, Yield Components and Grain Protein Content of Two Barley Cultivars. Journal of Crop Production and Processing, 6(20), 191-207. (In Persian with English abstract). https://doi.org/10.18869/acadpub.jcpp.6.20.191

Barati, V., Ghadiri, H., Zand-Parsa, S., & Karimian, N. (2015). Nitrogen and water use efficiencies and yieldresponse of barley cultivars under different irrigation and nitrogen regimes in a semi-arid mediterranean climate. Archives of Agronomy and Soil Science, 61(1), 15-32. https://doi.org/10.1080/03650340.2014.921286

Barea, J. M. (2015). Future challenges and perspectives for applying microbial biotechnology in sustainable agriculture based on a better understanding of plant-micro biome interactions. Journal of Soil Science and Plant Nutrition, 15(2), 261-282. https://doi.org/10.4067/s0718-95162015005000021

Barker, A. V. (2016). Science and Technology of Organic Farming. Emam Reza University, Mashhad, Iran. (In Persian).

Bedoussac, L., & Justes, E. (2011). Dynamic analysis of competition and complementarity for light and N use to understand the yield and the protein content of a durum wheat–winter pea intercrop. Plant and Soil, 330(1), 37-54. https://doi.org/10.1007/s11104-010-0303-8

Bhattacharjee, R., & Dey, U. (2014). Biofertilizer, a way towards organic agriculture: A review. African Journal of Microbiology Research, 8(24), 2332-2343. https://doi.org/10.5897/ajmr2013.6374

Bouzarjomehri, K., Khatami, S. S., Zarrin, A., & Falsolayman, M. (2022). Analysis of water resources management studies in Iran and the world. Journal of Geography and Environmental Hazards, 11(2). (In Persian with English abstract). https://doi.org/10.22067/geoeh.2022.73891.1136

Brooker, R. W., Bennett, A. E., Cong, W. F., Daniell, T. J., George, T. S., Hallett, P. D., Hawes, C., Iannetta, P. P., Jones, H. G., Karley, A. J., & Li, L. (2015). Improving intercropping: a synthesis of research in agronomy. New Phytologist, 206(1), 107-117. https://doi.org/10.1111/nph.13132

Cassan, F., & Diaz-Zorita, M. (2016). Azospirillum sp. in current agriculture: from the laboratory to the field. Soil Biology and Biochemistry, 103, 117-130. https://doi.org/10.1016/j.soilbio.2016.08.020

Chamkhi, I., Cheto, S., Geistlinger, J., Zeroual, Y., Kouisni, L., Bargaz, A., & Ghoulam, C. (2022). Legume-based intercropping systems promote beneficial rhizobacterial community and crop yield under stressing conditions. Industrial Crops and Products, 183, 114958. https://doi.org/10.1016/j.indcrop.2022.114958

Chapagain, T., & Riseman, A. (2014). Intercropping wheat and beans: effects on agronomic performance and land productivity. Crop Science, 54(5), 2285-2293. https://doi.org/10.2135/cropsci2013.12.0834

Cominelli, E., & Tonelli, C. (2010). Transgenic crops coping with water scarcity. New Biotechnology, 27(5), 473-477. https://doi.org/10.1016/j.nbt.2010.08.005

Diaz-Zorita, M., & Fernandez-Canigia, M. V. (2009). Field performance of a liquid formulation of Azospirillum brasilense on dryland wheat productivity. European Journal of Soil Biology, 45(1), 3-11. https://doi.org/10.1016/j.ejsobi.2008.07.001

Emam, Y. (2007). Cereal production. Shiraz University, Shiraz, Iran. (In Persian).

Ercoli, L., Lulli, L., Mariotti, M., Masoni, A., & Arduini, I. (2008). Post-anthesis dry matter and nitrogen dynamics in durum wheat as affected by nitrogen supply and soil water availability. European Journal of Agronomy, 28: 138-147. https://doi.org/10.1016/j.eja.2007.06.002

Farahmand, A., & Liaghat, A. (2010). Evaluation of soil nitrate contamination due to irrigation water and nitrogen fertilizer. Water Resources Engineering, 4(10), 85-92. (In Persian).

Feledyn-Szewczyk, B., Nakielska, M., Jonczyk, K., Berbec, A. K., & Kopinski, J. (2020). Assessment of the suitability of 10 winter triticale cultivars (× Triticosecale Wittmac) for organic agriculture: Polish case study. Agronomy, 10, 1144. https://doi.org/10.3390/agronomy10081144

Frederick, J. R., & Camberato, J. J. (1994). Leaf net CO₂-exchange rate and associated leaf traits of winter wheat grown with various spring nitrogen fertilization rates. Crop Science, 34, 432-439. https://doi.org/10.2135/cropsci1994.0011183x003400020024x

Garabet, S., Wood, M., & Ryan, J. (1998). Nitrogen and water effects on wheat yield in a Mediterranean type climate. I. Growth, water-use and nitrogen accumulation. Field Crops Research, 57, 309-318. https://doi.org/10.1016/s0378-4290(98)00075-6

Ghasemi, A., Ghanbari, A., Fakheri, B. A., & Fanaie, H. R. (2016). Effect of different fertilizer resources on yield and yield components of grain maize (Zea mays L.) influenced by tillage managements. Journal of Agroecology, 7(4), 499-512.

Grimes, D. W., Yamada, H., & Hughes, S. W. (1987). Climate-normalized cotton leaf water potentials for irrigation scheduling. Agricultural Water Management, 12(4), 293-304. https://doi.org/10.1016/0378-3774(87)90004-7

Haynes, R. J. (1980). Competitive aspects of the grass-legume association. Advances in Agronomy, 33, 227-261. https://doi.org/10.1016/s0065-2113(08)60168-6

Izan, T., Javanmard, A., Shekari, F., & Abbasi, A. (2020). Evaluation of Yield, Yield Components and Some Physiological Traits of Sunflower with Integrative Application of Biological, Chemical, and Organic Fertilizers under Different Irrigation Levels. Journal of Agricultural Science and Sustainable Production, 30(3), 87-111. (In Persian with English abstract).

Ledgard, S. F., & Steele, K. W. (1992). Biological nitrogen fixation in mixed legume/grass pastures. Plant and Soil, 141(1), 137-153. https://doi.org/10.1007/bf00011314

Liu, X., Liu, W., Tang, Q., Liu, B., Wada, Y., & Yang, H. (2022). Global agricultural water scarcity assessment incorporating blue and green water availability under future climate change. Earth's Future, 10, e2021EF002567. https://doi.org/10.1029/2021ef002567

Mazaheri, D. (1998). Intercropping. Tehran University, Tehran, Iran. (In Persian).

Mcgoverin, C. M., Snyders, F., Muller, N., Botes, W., Fox, G., & Manley, M. (2011). A review of triticale uses and the effect of growth environment on grain quality. Journal of the Science of Food and Agriculture, 91, 1155-1165. https://doi.org/10.1002/jsfa.4338

Moradi, M., Soleymanifard, A., Naseri, R., Ghasemi, M., & Abromand, K. (2016). The changes of agronomic traits and harvest index of wheat under the effect of manure and plant growth promotion bacteria at different levels of nitrogen. Crop Physiology Journal, 7(28), 73-90. (In Persian).

Munareto, J. D., Martin, T. N., Fipke, G. M., Cunha, V. D. S., & Rosa, G. B. D. (2019). Nitrogen management alternatives using Azospirillum brasilense in wheat. Pesquisa Agropecuária Brasileira, 54, e00276. https://doi.org/10.1590/s1678-3921.pab2019.v54.00276

Nabati, J., & Yousefi, A. (2019). Biological fertilizers approach for ecological compression. First Conference on Basic Research in Agricultural and Environmental Sciences. (In Persian with English abstract).

Naiman, A. D., Latronico, A., & de Salamone, I. E. G. (2009). Inoculation of wheat with Azospirillum brasilense and Pseudomonas fluorescens: impact on the production and culturable rhizosphere microflora. European Journal of Soil Biology, 45(1), 44-51. https://doi.org/10.1016/j.ejsobi.2008.11.001

Nasiri, Y., Musavi Zadeh, S. A., & Asadi, M. (2019). Effect of application of livestock, biological and chemical fertilizers on yield, yield components and some morphological characteristics of wheat. Agricultural Science and Sustainable Production, 30(1), 313-328. (In Persian).

Neugschwandtner, R. W., & Kaul, H. P. (2014). Sowing ratio and N fertilization affect yield and yield components of oat and pea in intercrops. Field Crops Research, 155, 159-163. https://doi.org/10.1016/j.fcr.2013.09.010

Niazi Ardakani, M., Barati, V., Bijanzadeh, E., & Behpouri, A. (2020). Effects of different nitrogen fertilizer sources and crop residues on yield and yield components of barley (Hordeum vulgare L.) under late season water stress. Journal of Agroecology, 12(1), 107-126. (In Persian with English abstract). https://doi.org/10.22067/jag.v12i1.79989

Pelzer, E., Hombert, N., Jeuffroy, M. H., & Makowski, D. (2014). Meta‐analysis of the effect of nitrogen fertilization on annual cereal-legume intercrop production. Agronomy Journal, 106(5), 1775-1786. https://doi.org/10.2134/agronj13.0590

Rezaei-Chiyaneh, E., Rasouli, Y., Jalilian, J., & Ghodsi, M. (2019). Evaluation of quantitative and qualitative yield of chickpea (Cicer arietinum L.) and barley (Hordeum vulgare L.) in intercropping affected by biological and chemical fertilizers in supplemental irrigation condition. Agroecology, 11(1).‏ (In Persian with English abstract).

Risse, L.M., Cabrera, M. L., Franzluebbers, A. J., Gaskin, J. W., Gilley, J. E., Killorn, R., Radcliffe, D. E., Tollner, W. E., & Zhang, H. (2006). Land application of manure for beneficial reuse. Biological Systems Engineering: Papers and Publications, 283-316.

Shata, S. M., Mahmoud, A., & Siam, S. (2007). Improving calcareous soil productivity by integrated effect of intercropping and fertilizer. Research Journal of Agriculture and Biological Science, 3(6), 733-739.

Sieling, K., Schroder, H., Finck, M., & Hanus, H. (1998). Yield, N uptake and apparent N use efficiency of winter wheat and winter barley grown in different cropping systems. The Journal of Agricultural Science, 131, 375-387. https://doi.org/10.1017/s0021859698005838

Singh, J. S., Pandey, V. C., and Singh, D. P. (2011). Efficient soil microorganisms: a new dimension for sustainableagriculture and environmental development. Agriculture, ecosystems and environment, 140(3-4), 339-353.

Sori, S., Amirnia, R., Rezaei-Chiyaneh, E., and Sheikh, F. (2020). Evaluation of Yield and Yield Components of Different Faba Bean (Vicia faba L.) Varieties in Intercropping with Triticale (Tritico secale). Journal Of Agroecology, 12(1), 143-159. https://doi.org/10.22067/jag.v12i1.81918

Tarang, E., Ramroudi, M., Galavi, M., Dahmardeh, M., & Mohajeri, F. (2013). Evaluation grain yield and quality of corn (Maxima CV) in responses to Nitroxin bioferilizer and chemical fertilizers. International Journal of Agriculture and Crop Sciences, 5, 683-687.

Tavakoli, M., & Jalali, A. H. (2016). Effect of different bio fertilizers and nitrogen fertilizer levels on yield and yield components of wheat. Journal of Crop Production and Processing, 6(21), 34-45. (In Persian with English abstract). https://doi.org/10.18869/acadpub.jcpp.6.21.34

Tien, T. M., Gaskins, M. H., & Hubbell, D. (1979). Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Applied and Environmental Microbiology, 37(5): 1016-1024. https://doi.org/10.1128/aem.37.5.1016-1024.1979

Van Herwaarden, A. F. (1996). Haying-off in wheat, enduring myth or current problem. In Proceedings of the 8^th Australian Agronomy Conf. CSIRO. ASA, Australia.

Van Herwaarden, A. F., Farquhar, G. D., Angus, J. F., Richards, R. A., & Howe, G. N. (1998). Haying-off, the negative grain yield response of dryland wheat to nitrogen fertilizer I. Biomass, grain yield, and water use. Australian Journal of Agricultural Research, 49, 1067-1081. https://doi.org/10.1071/a97039

Wojcik-Gront, E., & Studnicki, M. (2021). Long-term yield variability of triticale (× Triticosecale wittmack) tested using a cart model. Agriculture, 11, 92. https://doi.org/10.3390/agriculture11020092

Zadoks, J.C., Chang, T.T., & Konzak, C.F. (1974). A decimal code for the growth stages of cereals. Weed Research, 14, 415-421. https://doi.org/10.1111/j.1365-3180.1974.tb01084.x

Zia, R., Nawaz, M. S., Siddique, M. J., Hakim, S., & Imran, A. (2021). Plant survival under drought stress: Implications, adaptive responses, and integrated rhizosphere management strategy for stress mitigation. Microbiological Research, 242, 126626. https://doi.org/10.1016/j.micres.2020.126626