Evaluation of Quantitative and Qualitative Traits of the First and Second Cuttings of Peppermint (Mentha piperita L.) under the Influence of the Integrated Application of Conventional, Nano Fertilizers and Mycorrhizal

Document Type : Research Article

Authors

• A Ostadi
• A Javanmard
• M. R Morshedloo
• S Mola Ali Abasiyan

University of Maragheh

10.22067/gsc.v17i2.75005

Abstract

Introduction
     In order to protect biodiversity, especially medicinal herb, and crude herbal drugs from the wild origin, Biological Diversity Act (2002) and Rule (2004) were enforced which compelled the herbal industries and medicinal plant growers for conventional cultivation of medicinal herbs. However, intensive cultivation of these medicinal herbs and herbal drugs to meet the global demand has led to aggravated use of chemical fertilizers and pesticides and consequently questioning their safety and integrity. Moreover, total fertilizer nutrient (N+P2O5+K2O) consumption was estimated at 170.7 and 175.7 million tons in 2010 and 2011, respectively which estimated consumption of N, P, and K fertilizers is expected to increase from current consumption levels by 172%, 175%, and 150%, respectively by 2050. Excessive using of chemical fertilizers caused serious environmental issues globally such as reduction of plant diversity, instability of economic yield, an increase of pest and disease damages, intensification of soil erosion. These increasing concerns regarding the negative impacts of these systems on the environment and human health suggest that more effort is needed to develop sustainable agricultural systems with higher productivity and improved efficiency. Application of nano-fertilizers and biofertilizers such as arbuscular mycorrhizal fungi (AM) is regarded as one of the promising approaches to increasing crop productivity. Nano-materials (NMs) are defined as materials that with at least one dimension less than 100 which can improve one or more nutrients absorption for plants and enhance their growth and yields. The AM fungi are a fundamental group of soil micro-organisms, symbiotically associated with nearly 80% of plant species.
Peppermint (Mentha piperita L.), which is a hybrid between Mentha spicata and Mentha Aquatica belonging to a family, Lamiaceae, is one of the most important essential oil-bearing plants. The objective of this experiment was an evaluation of the quantity and quality traits of peppermint under the integrated application of chemical and nano-fertilizers and arbuscular mycorrhizal fungi.
 Materials and Methods
In order to investigate the effects of different fertilizers on the quantity and quality of peppermint at the first and second cuttings, a field experiment study was carried out as split-plot in time based on a randomized complete block design (RCBD) with seven treatments and three replications and two cutting times at the faculty of Agriculture, University of Maragheh, Iran, in 2017. The main factor was different fertilizer treatments included no fertilizer (control), chemical fertilizer (CF), arbuscular mycorrhiza fungi (AMF) (Glomus mussels), 50% CF+ AMF, Nano-fertilizers (NF), 50% NF+ 50% CF, NF+ AMF and the sub-factor was cutting time (first and second cutting). In AM fungi treatments, 80 g of the soil containing mycorrhizal fungi hyphae and the remains of the root and spores (1000 g spore.10-1 g soil) was added to soil in planting times. Also, Nitrogen Nano-fertilizer used with 2 ml/L concentration. Moreover, phosphorus, potassium, iron and zinc Nano-fertilizers applied with 1 g.L-1 concentration. Furthermore, manganese Nano-fertilizer used in 0.5 g/L concentration. Additionally, the foliar application of Nano-fertilizers was carried out in two-stage (One month after the cultivation and one month after first cutting).
 Results and Discussion
The results demonstrated that the highest total dry matter yield (382.6 g.m-2) was achieved under 100% CF followed by 50% CF+ AMF and 50% NF+ 50% CF treatments, respectively. On the other hand, the highest (6.1 g.m-2) and lowest (2.9 g.m-2) essential oil yield were obtained under 50% CF+ AMF and control, respectively. Chemical analysis of the essential oil demonstrated that in all treatments, menthol, menthone, 1, 8 cineole, neo-iso menthol, neo-menthol, Menthofuran, and p-Menth-1-en-9-ol were the main dominant components. The highest amount of menthol was obtained in nano-fertilizer treatment and at the second cutting. Furthermore, monoterpene hydrocarbons were the major fraction of the essential oil in both harvests.
 Conclusions
Based on the results, integrative application of 50% CF+ AMF and 50% CF+ 50% NF had a higher significant impact on the morphological traits compared with other treatments. The highest dry matter yield was achieved in the second cutting with the application of CF that was not significantly impacted with 50% CF+ AMF and 50% CF+ 50% NF treatments. Also, the highest essential oil content was obtained the integrative application of 50% CF+ 50% NF that was 7.1% higher than CF in the first cutting. In addition, the essential oil yield productivity increased 198% with application of 50% CF+ AMF and 50% CF+ 50% NF compared with control. Based on the essential oil compounds, the highest (46.31%) and the lowest (26.73%) content of menthol was recorded in the second cutting fertilized with NF and first cutting fertilized with CF. Generally, based on the obtained results, integrative application of AMF and NF with 50% CF increased the quality and quantity productivity of peppermint compared with 100% CF.
 

Keywords

• Harvest
• Integrated fertilizers application
• Menthol
• Menthone
• Sustainable agriculture

20.1001.1.20081472.1398.17.2.11.1

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References

1. Abdullahi, R., and Sheriff, H. 2013. Effect of arbuscular mycorrhizal fungi and chemical fertilizer on growth and shoot nutrients content of onion under field condition in Northern Sudan Savanna of Nigeria. Journal of Agriculture and Veterinary Science 3 (5): 85-90.

2. Adams, R. P. 2017. Identification of essential oil components by gas chromatography/mass spectrometry. Allured publishing corporation. (4): 1-804 : Allured Publishing Corporation Carol Stream, IL.

3. Amani Machiani, M., Javanmard, A., Morshedloo, M. R., and Maggi, F. 2018. Evaluation of yield, essential oil content and compositions of peppermint (Mentha piperita L.) intercropped with faba bean (Vicia faba L.). Journal of Cleaner Production 171: 529-537.

4. Amuamuha, L., Pirzad, A., and Hadi, H. 2012. Effect of varying concentrations and time of Nanoiron foliar application on the yield and essential oil of Pot marigold. International Research Journal of Applied and Basic Sciences 3 (10): 2085-2090.

5. Aslani, Z., Hassani, A., Rasooli, S. M., Sefidkon, F., and Barin, M. 2011. Effect of two fungi species of arbuscular mycorrhizal (Glomus mosseae and Glomus intraradices) on growth, chlorophyll contents and P concentration in Basil (Ocimum basilicum L.) under drought stress conditions. Iranian Journal of Medicinal and Aromatic Plants Research 27 (3): 471-486. (in Persian with English abstract).

6. Azizi, A., Yan, F., and Honermeier, B. 2009. Herbage yield, essential oil content and composition of three oregano (Origanum vulgare L.) populations as affected by soil moisture regimes and nitrogen supply. Industrial Crops and Products 29 (2-3): 554-561.

7. Babaei, K., Seyed Sharifi, R., Pirzad, A., and Khalilzadeh, R. 2017. Effects of bio fertilizer and nano Zn-Fe oxide on physiological traits, antioxidant enzymes activity and yield of wheat (Triticum aestivum L.) under salinity stress. Journal of Plant Interactions 12 (1): 381-389.

8. Bahadori, F., Ashorabadi, E. S., Mirza, M., Matinizade, M., and Abdosi, V. 2013. Improved growth, essential oil yield and quality in Thymus daenensis Celak on mycorrhizal and plant growth promoting rhizobacteria inoculation. International Journal of Agronomy and Plant Production 4: 3384-3391.

9. Baranauskienė, R., Venskutonis, P. R., Viškelis, P., and Dambrauskienė, E. 2003. Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris). Journal of Agricultural and Food Chemistry 51 (26): 7751-7758.

10. Baum, C., El-Tohamy, W., and Gruda, N. 2015. Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi: a review. Scientia Horticulturae 187: 131-141.

11. Cabello, M., Irrazabal, G., Bucsinszky, A. M., Saparrat, M., and Schalamuk, S. 2005. Effect of an arbuscular mycorrhizal fungus, Glomus mosseae, and a rock‐phosphate‐solubilizing fungus, Penicillium thomii, on Mentha piperita growth in a soilless medium. Journal of Basic Microbiology: An International Journal on Biochemistry, Physiology, Genetics, Morphology, and Ecology of Microorganisms 45 (3): 182-189.

12. Court, W. A., Roy, R. C., Pocs, R., More, A. F., and White, P. H. 1993. Optimum Nitrogen Fertilizer Rate for Peppermint (Mentha piperita L.) in Ontario, Canada. Journal of Essential Oil Research 5 (6): 663-666.

13. Elfeky, S. A., Mohammed, M. A., Khater, M. S., Osman, Y. A. and Elsherbini, E. 2013. Effect of magnetite Nano-Fertilizer on Growth and yield of Ocimum basilicum L. International Journal of Indigenous Medicinal Plants 46 (3): 1286-1293.

14. Francis, C. A., Bulter, F. C., and King, L. D. 2000. Crop growth and relative growth rates in (Matricaria chamomilla L.). Crop Science 88: 1207-1212.

15. Galvan, G. A., Paradi, I., Burger, K., Baar, J., Kuyper, T. W., Scholten, O. E., and Kik, C. 2009. Molecular diversity of arbuscular mycorrhizal fungi in onion roots from organic and conventional farming systems in the Netherlands. Mycorrhiza 19 (5): 317-328.

16. Geneva, M. P., Stancheva, I. V., Boychinova, M. M., Mincheva, N. H., and Yonova, P. A. 2010. Effects of foliar fertilization and arbuscular mycorrhizal colonization on Salvia officinalis L. growth, antioxidant capacity, and essential oil composition. Journal of the Science of Food and Agriculture 90 (4): 696-702.

17. Giovannetti, M., and Mosse, B. 1980. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist 84 (3): 489-500.

18. Gryndler, M., Hršelova, H., Sudova, R., Gryndlerova, H., Řezačova, V., and Merhautova, V. 2005. Hyphal growth and mycorrhiza formation by the arbuscular mycorrhizal fungus Glomus claroideum BEG 23 is stimulated by humic substances. Mycorrhiza 15 (7): 483-488.

19. Gutierrez‐Mañero, F. J., Ramos‐Solano, B., Probanza, A., Mehouachi, J., R Tadeo, F., and Talon, M. 2001. The plant‐growth‐promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiologia Plantarum 111 (2): 206-211.

20. Hagh Parast Tanha, M. 1992. Nutrition and Metabolism Plants. Islamic Azad University Publishing, Rasht.

21. Hassiotis, C. N., Ntana, F., Lazari, D. M., Poulios, S., and Vlachonasios, K. E. 2014. Environmental and developmental factors affect essential oil production and quality of Lavandula angustifolia during flowering period. Industrial Crops and Products 62: 359-366.

22. Hofmeyer, P. V., Seymour, R. S., and Kenefic, L. S. 2010. Production ecology of Thuja occidentalis. Canadian Journal of Forest Research 40 (6): 1155-1164.

23. Izadi, Z., Ahmadvand, G., Asna-Ashari, M., and Piri, K. 2010. The effect of nitrogen and plant density on some growth characteristics, yield and essential oil in peppermint (Mentha piperita L.). Iranian Journal of Field Crops Research 8 (5): 827-836. (in Persian with English abstract).

24. Kahiluoto, H., Ketoja, E., and Vestberg, M. 2009. Contribution of arbuscular mycorrhiza to soil quality in contrasting cropping systems. Agriculture, Ecosystems & Environment 134 (1-2): 36-45.

25. Kapoor, R., Anand, G., Gupta, P., and Mandal, S. 2017. Insight into the mechanisms of enhanced production of valuable terpenoids by arbuscular mycorrhiza. Phytochemistry Reviews 16 (4): 677-692.

26. Kapoor, R., Chaudhary, V., and Bhatnagar, A. 2007. Effects of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua L. Mycorrhiza 17 (7): 581-587.

27. Kapoor, R., Giri, B., and Mukerji, K. G. 2004. Improved growth and essential oil yield and quality in Foeniculum vulgare mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technology 93 (3): 307-311.

28. Karagiannidis, N., Thomidis, T., Lazari, D., Panou-Filotheou, E., and Karagiannidou, C. 2011. Effect of three Greek arbuscular mycorrhizal fungi in improving the growth, nutrient concentration, and production of essential oils of oregano and mint plants. Scientia Horticulturae 129 (2): 329-334.

29. Khan, M., Mobin, M., Abbas, Z., and Alamri, S. 2018. Fertilizers and their contaminants in soils, surface and groundwater. Encyclopedia Anthropocene 5: 225-240.

30. Khater, M. S. 2015. Magnetite-Nanoparticles Effects on Growth and essential oil of Peppermint. Current Science International 4 (2): 140-144.

31. Koske, R., and Gemma, J. 1989. A modified procedure for staining roots to detect VA mycorrhizas. Mycological Research 92 (4): 486-488.

32. Liu, R., and Lal, R. 2015. Potentials of engineered nanoparticles as fertilizers for increasing agronomic productions. Science of the Total Environment 514: 131-139.

33. Mafakheri, S., Asghari, B., and Shaltooki, M. 2016. Effects of biological, chemical and nano-fertilizers on quantitative and qualitative characteristics of Lallemantia iberica (MB) Fischer & Meyer. Iranian Journal of Medicinal and Aromatic Plants Research 32 (4): 667-677.

34. Mandal, S., Upadhyay, S., Wajid, S., Ram, M., Jain, D. C., Singh, V. P., Abdin, M. K., and Kapoor, R. 2015. Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels. Mycorrhiza 25 (5): 345-357.

35. Marcum, D. B., and Hanson, B. R. 2006. Effect of irrigation and harvest timing on peppermint oil yield in California. Agricultural Water Management 82 (1): 118-128.

36. Masoumi Zavarian, A., Yousefi Rad, M., and Asghari, M. 2015. Effects of Mycorrhizal Fungi on Quantitative and Qualitative Characteristics of Anise Plant (Pimpinella anisum) under Salt Stress. Journal of Medicinal Plants 4 (56): 139-148.

37. McGonigle, T., Miller, M., Evans, D., Fairchild, G., and Swan, J. 1990. A new method which gives an objective measure of colonization of roots by vesicular arbuscular mycorrhizal fungi. New Phytologist 115 (3): 495-501.

38. Mishra, P., Singh, A., Mishra, R. R., Ong, S., and Prasad, S. M. 2018. Application of Nanotechnology to Enhance the Nutrient Quality of Food Crops and Agricultural Production Nanomaterials in Plants, Algae, and Microorganisms (pp. 453-472): Elsevier.

39. Moghadam, E., Mahmoodi, S. M., Farrokhian, F. A., Ramazani, Z., and Eskandari, F. 2015. The effect of foliar application of iron chelate type on morphological traits and essential oil content of holy basil (Ocimum sanctum). Journal of Crops Improvement 17 (3): 595 -606.

40. Morshedloo, M. R., Craker, L. E., Salami, A., Nazeri, V., Sang, H., and Maggi, F. 2017. Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono-and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies. Plant Physiology and Biochemistry 111: 119-128.

41. Morshedloo, M. R., Maggi, F., Neko, H. T., and Aghdam, M. S. 2018. Sumac (Rhus coriaria L.) fruit: Essential oil variability in Iranian populations. Industrial Crops and Products 111: 1-7.

42. Mura, S., Seddaiu, G., Bacchini, F., Roggero, P. P., and Greppi, G. F. 2013. Advances of nanotechnology in agro-environmental studies. Italian Journal of Agronomy 8 (3): 127-140.

43. Ormeño, E., and Fernandez, C. 2012. Effect of soil nutrient on production and diversity of volatile terpenoids from plants. Current Bioactive Compounds 8 (1): 71-79.

44. Pavela, R., Žabka, M., Vrchotova, N., and Třiska, J. 2018. Effect of foliar nutrition on the essential oil yield of Thyme (Thymus vulgaris L.). Industrial Crops and Products 112: 762-765.

45. Peter, K. 2006. Handbook of herbs and spices. Woodhead Publishing.

46. Phillips, J. M., and Hayman, D. 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society 55 (1): 158-161.

47. Poshtdar, A., Mashhadie, A., Moradi, F., Siadat, S., and Bakhshandeh, A. 2016. Effect of source and rate of nitrogen fertilizer on yield and water and nitrogen use efficiency of peppermint (Mentha piperita L.). Iranian Journal of Crop Sciences 18 (1): 14-31. (in Persian with English abstract).

48. Rahmani, N., Valadabadi, S. A., Daneshian, J., and Bigdeli, M. 2008. The effects of water deficit stress and nitrogen on oil yield of Calendula officinalis L. Iranian Journal of Medicinal and Aromatic Plants Research 24 (1): 103-108.

49. Razavi, S., Jahan, M., Mahallti, M., and Ghalibaf, K. 2017. Radiation absorption and use efficiency of common mallow (Malva sylvestris L.) affected by different sources of organic, biological and chemical fertilizers and intercropping with fenugreek (Trigonella foenum-graecum). Iranian Journal of Field Crops Research 15 (1): 136-149.

50. Shajari Aghhavani, M., Rezvani Moghaddam, P., Ghorbani, R., and Nasiri Mahallati, M. 2016. Effects of single and combined application of organic, biological and chemical fertilizers on quantitative and qualitative yield of coriander (Coriandrum sativum). Journal of Horticultural Science 29 (4): 486-500. (in Persian with English abstract).

51. Smith, S., and Read, D. 2008. Mycorrhizal Symbiosis 3rd edn Academic Press: San Diego. CA, USA, 787.

52. Smitha, G., Basak, B., Thondaiman, V., and Saha, A. 2019. Nutrient management through organics, bio-fertilizers and crop residues improves growth, yield and quality of sacred basil (Ocimum sanctum Linn). Industrial Crops and Products 128: 599-606.

53. Solanki, P., Bhargava, A., Chhipa, H., Jain, N., and Panwar, J. 2015. Nano-fertilizers and their smart delivery system Nanotechnologies in food and agriculture (pp. 81-101): Springer.

54. Tarraf, W., Ruta, C., Tagarelli, A., De Cillis, F., and De Mastro, G. 2017. Influence of arbuscular mycorrhizae on plant growth, essential oil production and phosphorus uptake of Salvia officinalis L. Industrial Crops and Products 102: 144-153.

55. Telci, I., Kacar, O., Bayram, E., Arabacı, O., Demirtaş, İ., Yılmaz, G., Özcan, I., Sönmez, C., and Göksu, E. 2011. The effect of ecological conditions on yield and quality traits of selected peppermint (Mentha piperita L.) clones. Industrial Crops and Products 34 (1): 1193-1197.

56. Urcoviche, R. C., Gazim, Z. C., Dragunski, D. C., Barcellos, F. G., and Alberton, O. 2015. Plant growth and essential oil content of Mentha crispa inoculated with arbuscular mycorrhizal fungi under different levels of phosphorus. Industrial Crops and Products 67: 103-107.

57. Valentine, A., Osborne, B., and Mitchell, D. 2001. Interactions between phosphorus supply and total nutrient availability on mycorrhizal colonization, growth and photosynthesis of cucumber. Scientia Horticulturae 88 (3): 177-189.

58. Varma, A., Prasad, R., and Tuteja, N. 2018. Mycorrhiza-Nutrient Uptake, Biocontrol, Ecorestoration. Springer.

59. Weisany, W., Sohrabi, Y., Siosemardeh, A., and Ghassemi-Golezani, K. 2017. Funneliformis mosseae fungi changed essential oil composition in Trigonella foenum graecum L., Coriandrum sativum L. and Nigella sativa L. Journal of Essential Oil Research 29 (3): 276-287.

60. Willmann, M., Gerlach, N., Buer, B., Polatajko, A., Nagy, R., Koebke, E., Jansa, J., Flisch, R., and Bucher, M. 2013. Mycorrhizal phosphate uptake pathway in maize: vital for growth and cob development on nutrient poor agricultural and greenhouse soils. Frontiers in Plant Science 4: 1-6.

61. Yousefzadeh, S., Modarres, S. S. A. M., Sefidkon, F., and Ghiasy, O. M. 2016. Effect of biofertilizer, azocompost and nitrogen on oil yield and essential oil content of Dracocephalum moldavica L. Iranian Journal of Horticultural Sciences 46 (4): 604-611.

62. Zehtab-Salmasi, S., Heidari, F., and Alyari, H. 2008. Effects of microelements and plant density on biomass and essential oil production of peppermint (Mentha piperita L.). Plant Science Research 1 (1): 24-26.

63. Zeinali, H., Hosseini, H., and Shirzadi, M. 2014. Effects of nitrogen fertilizer and harvest time on agronomy, essential oil and menthol of Mentha piperita L. Iranian Journal of Medicinal and Aromatic Plants Research 30 (3): 486-495.

64. Zheljazkov, V. D., Cerven, V., Cantrell, C. L., Ebelhar, W. M., and Horgan, T. 2009. Effect of nitrogen, location, and harvesting stage on peppermint productivity, oil content, and oil composition. American Society for Horticultural Science 44 (5): 1267-1270.

65. Zolfaghari, M., Nazeri, V., Sefidkon, F., and Rejali, F. 2013. Effect of arbuscular mycorrhizal fungi on plant growth and essential oil content and composition of Ocimum basilicum L. Iranian Journal of Plant Physiology 3 (2): 643-650.