Investigating the Application of Phosphorus and Spraying of Micronutrients on Forage and Seed Yield in Alfalfa Ecotypes

Document Type : Research Article

Authors

Isfahan Agricultural and Natural Resources Research and Education Center

Abstract

Introduction
Alfalfa (Medicago sativa L.) is known as the most valuable forage in the world due to its resistance to drought, the high quality of forage and high adaptability, high shelf life and high protein. Desirable nutrition of alfalfa is one of the key factors in the quantity and quality of seed and forage production. In alfalfa production, optimum nutrition is one of the key factors in the growth and development of the product and has a direct effect on the yield of forage and seed. The provision of phosphorus and micronutrient elements of soil in order to balance the nutrients of soil plays an important role in quantitative and qualitative improvement of the product, and it is necessary to carry out a comprehensive research on alfalfa. The purpose of this study was to investigate the effect of different levels of phosphorus and micronutrient fertilizer on quantitative and qualitative yield of alfalfa seed and forage.
Material and Methods
In a three-year research (2011-2014), the effects of using phosphorus and micronutrients including iron, manganese, copper and zinc on quantitative and qualitative characteristics of seeds and forage production of alfalfa were studied at Golpayegan Research Station Isfahan province. For this purpose, using a split- plot experiment based on a randomized complete block design with three replications, the effect of two alfalfa ecotypes (as the main plot) and 6 levels of phosphorus and micronutrient fertilization (as a sub-plot) on the yield of seed and forage production was investigated. The main plots were consisted of alfalfa Hamedani (V1) and Rehnani (V2) and sub plots (sub-treatment) were included phosphorus (P2O5) at three levels: zero (P0), 65 (P65) and 120 (P120) kg.ha-1, and micronutrient elements in two levels of no applied (R0) and micronutrient application as spraying (R1). Parameters were measured, including forage weight, dry forage weight, seed yield, 1000 seed weight and seed vigor percentage. In the first year, there were two cutting for forage and one for seed production, in the second year there were three cutting for forage and one for seed production, and in the third year there were three cutting for forage production and one for seed production.
Results and Discussion
The results showed that there was no significant difference in forage production, seed yield, 1000 seed weight, and percentage of seed vigor between two ecotypes of alfalfa, and the average yield of forage and seed in Hamedi and Rehnani ecotypes was 35.40 and 34.77 ton.ha-1 and 498 and 487 kg.ha-1 respectively. The year had a significant effect on the yield of fresh and dry forage, seed yield and 1000-seed weight. Also, the maximum of these traits were observed in the second year. The effect of the year on vigor seed was not significant. Comparison of means showed that P fertilizer application of 120 kg.ha-1 with micronutrient spraying (P120-R1) had the highest seed production and increased of 105% compared to control treatment. The application of 120 kg.ha-1 phosphorus without micronutrient application was in the next place. The percentage of seed vigor in the treatment of 120 kg.ha-1 P, with the micronutrient application (95.1%) was significantly higher than other treatments.
Conclusions
In this three-year research, the study of the use of phosphorus and micronutrients on fresh and dry forage yield and some quantitative and qualitative characteristics of seed produced in two alfalfa ecotypes was carried out. The results showed that the highest amount of forage production with the amount of 42.45 ton.ha-1 and the highest amount of seed produced with the amount of 689 kg.ha-1, belonged to the treatment 120 kg.ha-1 phosphorus with micronutrient spraying.

Keywords


1. Alizadeh, A., and Kamali, G. H. 2007. Water requirement of plants in Iran. Imam Reza University Press. First Printing P: 223.
2. Al-Kahtani, S. N., El-Kazafy, A. T., and Al-Abdulsalam, M. 2017. Alfalfa (Medicago sativa L.) seed yield in relation to phosphorus fertilization and honeybee pollination. Saudi Journal of Biological Sciences 24: 1051-1055.
3. Anonymous. 1985. International Rules, for Seed Testing. Seed Science and Technology 13 (2): 299-513.
4. Bai, B. Z. 1992. Plant physiology. Beijing: China Agricultural Press P: 183.
5. Baligar, V. C., and Fageria, N. K. 2005. Soil aluminum effects on growth and nutrition of Cacao. Soil Science and Plant Nutrition 51: 709-713.
6. Berg, W. K., Cunningham, S. M., Brouder, S. M., Joern, B. C., Johnson, K. D., Santini, J., and Volenec, J. J. 2005. Influence of phosphorus and potassium on alfalfa yield and yield components. Crop Science Abstract – Forage and Grazing Lands 45 (1): 297-304.
7. Bolanos-Aguilar, E. D., Huyghe, C., Julier, B., and Ecalle, C. 2000. Genetic variation for seed yield and its components in alfalfa (Medicago sativa L.) Populations. Agronomy 20 (3): 335-345.
8. Brown, B., and Gibson, R. 2000. Nutrient availability for alfalfa seed production. Project No. BJK-214. Brad Brown, Parma Research and Extension Center, 29603 U of I Lane, Parma, Idaho 83660.
9. Davis, J. G., Smithand, D. H., and Croissant, R. L. 2005. Fertilizing alfalfa and grasses. Colorado State University, Cooperative Extension 3/96.
10. Dordas, C. H. 2006. Foliar boron application improves seed set, seed yield and seed quality of alfalfa. Agronomy Journal 98 (4): 907-913.
11. Du, W. H., Tian, X. H., Cao, Z. Z., and Humphries, A. 2009. Effects of micronutrients on seed yield and yield components of alfalfa. Journal of Plant Nutrition 32 (5): 809-820.
12. Habibi, H., Aghighi Shahverdi, M., Nasiri, Z., Chaichi, M. R., and Fotokian, M. H. 2015. Effect of seed rate and efficiency of PGPR on quality of alfalfa (Medicago sativa L.) seed, using standard germination and accelerated. Iranian Journal of Seed Research 1 (2): 1-17. (in Persian with English abstract)
13. Hall, M. H., Jennings, J. A., and Shewmaker, G. E. 2004. Alfalfa establishment guide. Available at http://www.plantmanagementnetwork.org/sub/fg/management/2004/alfalfa/Hall.pdf (verified 24 Apr. 2008. Forage and Grazinglands. DOI:10.1094/FG-2004-0723-01-MG.
14. Hashemi Majd, K., Mohammadi Farani, T., Homaporgurabajiri, M., Yaghoubi, A., Kuckakpur, Sh., Komaklaei, F., and Abdollahi, J. 2009. Determination of critical phosphorus limit for alfalfa in Ardabil area soils. 11th Iranian Soil Science Congress, Gorgan, University of Gorgan. (in Persian).
15. Malakouti, M. J., Nafisi, M., and Motshrazadeh, B. 2001. National determination to produce fertilizer inside the country is a valuable step toward self-sufficiency and access to sustainable agriculture. Agricultural Research and Education Organization. Soil and Water Research Institute. Agriculture Education Publication. Tehran, Iran. P: 300. (in Persian).
16. Malakouti, M. J., Tabatabai, J., and Kasafi, M. 2005. New methods for timely supply of nutrients in plants. Department of Horticulture, Ministry of Jihad-e-Agriculture. Tehran, Iran. (in Persian).
17. Markarian, Sh., Najafi, N., Asgharzadeh, N. A., and Avesta, Sh. 2016. Interactive effects of Ensifer Meliloti (Sinorhizobium meliloti) and phosphorus on some growth characteristics of alfalfa under soil water deficit conditions. Journal of Soil Biology 3 (2): 163-177. (in Persian with English abstract).
18. Mehnatkesh, A. 2005. Effect of soil nutrient utilization on nutrient yield and quality characteristics of alfalfa. 9th Soil Science Congress of Iran. Soil Conservation and Watershed Management Research Center, Tehran. (in Persian).
19. Moeinizadeh, M., Piri, E., Tavasoli, A., and Shojaei, S. 2017. Study of quantitative and qualitative forage yield of alfalfa cultivars (Medicago sativa L.) in different harvest in Khash region. Journal of Applied Research of Plant Ecophysiology 3 (2): 127-140. (in Persian with English abstract).
20. Moyer, J. R. 1992. Alfalfa yields in establishment and subsequent years after herbicide and phosphorus application during establishment. Canadian Journal of Plant Science 72 (2): 619-625.
21. Mullen, R. W., Johnson, G. V., Stritzke, J. F., Caddel, J. L., Phillips, S. B., and Raun, W. R. 2000. Alfalfa yield response to method and rate of applied phosphorus. Better Crops 84 (3): 18-23.
22. Nabiei, M. Gh. 2001. Effect of phosphate on forage yield of different alfalfa cultivars in rain fed condition. Iranian Journal of Rangeland and Desert Research 8 (4): 25-34. (in Persian with English abstract).
23. Ottman, M. 2010. Alfalfa nutrient requirements, deficiency symptoms and fertilizer application. 2010 California Alfalfa & Forage Symposium, Visalia Convention Center. Visalia, CA.
24. Sengul, S. 2006. Using path analysis to determine lucerne (Medicago sativa L.) seed yield and its components. New Zealand Journal of Agricultural Research 49 (1): 107-115.
25. Shabani, G., Chaichi, M. R., Ardakani, M. R., Khavazi, K., and Friedel, J. 2014. The effect of different fertilizing systems on seed yield and phosphorous absorption in annual medic var. Robinson. Applied Field Crops Research 27 (104): 87-95. (in Persian with English abstract).
26. Torabi, M. 2001. Investigating and determining the most suitable seed rats and cutting time before seeding of alfalfa. Agricultural Research, Education and Extension Organization (AREEO). Final Report No. 806/79. (in Persian).
27. Zhang, T., Kang, J., Zhao, Z., Guo, W., and Yang, Q. 2014. Frequency, depth and rate of phosphorus fertilizer application effects on alfalfa seed yields. Canadian Journal of Plant Science 94 (7): 1149-1156.
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  • Receive Date: 17 December 2018
  • Revise Date: 01 September 2019
  • Accept Date: 14 December 2019
  • First Publish Date: 20 March 2020