The Effect of Seed Treatment and Foliar Spray with Ellagic Acid on Physiological Traits of Plants Soybean under Accelerated Aging

Document Type : Research Article

Authors

S Arab ¹

M Baradaran Firouzabadi ²

A Gholami ²

M Haydari ²

¹ Ph D. Student of Agronomy, Shahrood University of Technology, Shahrood, Iran

² Department of Agronomy, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran

10.22067/jcesc.2022.74387.1131

Abstract

Introduction
Aging seed can be defined as loss of seed quality, viability and vigor. This process is irreversible over time and its intensity increases with increasing temperature and humidity. Aging seed is one of the major factors reducing quality and quantity in the agricultural sector, especially oilseeds. The use of antioxidants can reduce the damaging effects of aging. Ellagic acid is a natural plant antioxidant that can play a role in reducing the effects of stress on plants. This substance is a phenolic compound and among different antioxidants, due to its polyphenolic properties, it is one of the best scavengers for oxygen free radicals.
Materials and Methods
To investigate the effect of ellagic acid on improving the quality of plants obtained from normal and aged soybean seeds, an experiment was conducted in the research farm of Shahroud University of Technology during the two cropping years 2019 and 2020. In this study two sets of seeds including control seeds (un-aged) and aged seed were used and ellagic acid (50 mg. l^-1) was applied for control, seed pretreatment, foliar spray and seed pretreatment+foliar spray. Farm research was conducted a factorial experiment based on a randomized complete block design (RCBD) in three replications. Soybean seeds var. DPX were collected from Mazandaran agricultural research center. The water content of seeds was 12%. The seeds were those that harvested in the same year and kept in a controlled storage room at the temperature between 14 to 17 °C and relative humidity of 30 to 40% at the Agricultural Research and Training Center and Natural Resources of Mazandaran Province. To prepare aged seeds in the laboratory, the seeds were incubated at 41°C and 95% relative humidity for 72 hours. Seed pretreatment with a concentration of 50 mg.L^-1 ellagic acid was performed under ventilation condition for 6 hours. The seeds were then dried in the shade and used to continue testing and measuring traits compared to normal seeds. Foliar application was done early in the morning at the beginning of flowering (R1) and in favorable environmental conditions.
Results and Discussion
The results showed that aging reduced the mean daily seedling field emergence by 61.39% compared to normal seeds. The ratio of chlorophyll a to b, total chlorophyll, stomatal conductance and membrane stability index in the leaves of plants from aged seeds decreased by 9.9, 6.3, 7.0 and 28.9%, respectively, compared to the control. The amount of free amino acids and soluble sugars in the leaves of plants from aged seeds increased compared to normal seeds. Pretreatment of normal and aged seeds with ellagic acid increased the mean daily seedling field emergence by 23.1% and 19. 7%, respectively. The content of insoluble sugars were increased with application of both pretreatment and foliar spray of ellagic acid 32.0% respectively. The highest oil yield in both conditions was observed with application of both pretreatment and foliar spray of ellagic aicd. The yield seed increased with ellagic acid compared to the control 23.6%. Grain yield was higher than 23.2% in the plants that had received ellagic acid in a form foliar spray. In the end, the pretreatment+foliar spray of ellagic acid had the highest grain yield, which increased to control 55.5%.
Conclusion
However, in this study, the application of ellagic acid as a seed pretreatment and foliar spray had a positive effect under normal and aging conditions, but in the scope of the research, the combined application of ellagic acid as seed pretreatment and foliar spraying can be recommended to increase seed yield and seed oil yield in soybean. In general, ellagic acid can be introduced as a powerful antioxidant in aging conditions to reduce some of the destructive effects of the reactions involved in aging.

Keywords

Antioxidant

Grain yield

Seedling emergence

20.1001.1.20081472.1401.20.3.5.8

Subjects

• Plant Physiology

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References

1. Abu El Soud, W., Hegab, M. M., Abdelgavad, H., Zinta, G., and Asard, H. 2013. Ability of ellagic acid to alleviate osmotic stress on chickpea seedlings. Journal of Plant Physiology and Biochemistry 71 (1): 173-183. https://doi.org/10.1016/j.plaphy.2013.07.007
2. Akbari, M., Baradaran firouzabadi, M., Amerian, M. R., and Farrokhi, N. 2019. The effect of foliar application and seed pretreatment with cinnamic acid on physiological characteristics of Cowpea (Vigna unguiculata) aged seeds and resulting plants. PhD Dissertation, Faculty of Agriculture, Shahrood University of Technology, Iran. (in Persian with English abstract).
3. Arab, S., Baradaran Firouzabadi, M., Gholami, A., and Haydari, M. 2021. Physiological responses of soybean plant (DPX) to pretreatment and foliar application of seaweed extract (Ascophyllum nodosum) and seed primary quality. Iranian Journal of Field Crops Research 20 (1): 105-119. (in Persian with English abstract). https://doi. 22067/jcesc.2021.74004.1119
4. Bagheri, H., Andalibi, B., and Azimi moghaddam, M. R. 2014. The effect of foliar application of atrazine on stomatal and nonstomatal control factors of photosynthesis in safflower in drought stress condition. Journal of Plant Research (Iranian Journal of Biology) 27 (2): 168-179. (in Persian with English abstract). https://doi. 20.1001.1.23832592.1393.27.2.2.2
5. Barranco, D., Ruiz, N., and Gomes, M. 2005. Frost tolerance of eight olive cultivars. European Journal of Horticultural Science 40: 558-560.
6. Bonnett, G. D., and Incoll, L. D. 1992. Effects on the stem of winter barley of manipulating the soucrose and sink during grain-filling1. Changes in accumulation and loss of mass from intemodes. Journal of Experimental Botany 44: 75-82. https://doi.org/10.1093/jxb/44.1.83
7. Braunberger, C., Zehl, M., Conrad, J., Fischer, S., Adhamib, H. R., Beifuss, U., and Krenn, L. 2013. C–MR, NMR, and LC–MS identification and LC– DAD quantification of flavonoids and ellagic acid derivatives in Droserapeltata. Journal of Chromatography 932: 111-116. https://doi.org/10.1016/j.jchromb.2013.06.015
8. Debnath, B., Singh, W. S., Das, M., Goswami, S., and Manna, K. 2021. Biodynamic activities of ellagic acid: A Dietary Polyphenol. Journal of Nature and Science of Medicine 3 (20): 83-90. DOI: 4103/JNSM.JNSM_32_19
9. Evtyugin, D., Magina, S., and Evtuguin, D. 2020. Recent advances in the production and applications of ellagic acid and its derivatives. A Review. Journal of Molecules 25: 27-45. https:// doi: 10.3390/molecules25122745
10. Hiscox, J. D., and Israelstom, G. F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1332-1334. https://doi.org/10.1139/b79-163
11. ISTA (International Seed Testing Association). 2009. International rules for seed testing. Seed Science and Technology 49: 86-41.
12. ISTA (International Seed Testing Association). 2006. International rules for seed testing. Basserdorf, Switzerland, 379 p.
13. Kalali, T., Lahooti, M., and Mahmoodzade, H. 2015. Investigation the effect of salicylic acid on morphological and physiological traits of soybean (Glycine max) under drought tension conditions. Crop Physiology Journal. 7(25): 75-88.
14. Kapoor, R., Arya, A., Siddiqui, M. A., Amir, A., and Kumar, H. 2010. Seed deterioration in chickpea (Cicer arietinum) under accelerated ageing. Asian Journal of Plant Sciences 9 (3): 158-162. DOI: 10.3923/ajps.2010.158.162
15. Khan, A., Nazar, S., Lang, I., Nawaz, H., and Hussain, M. A. 2017. Effect of ellagic acid on growth and physiology of canola (Brassica napus) under saline conditions. Journal Plant Interaction 12 (1): 520-525. https://doi.org/10.1080/17429145.2017.1400122
16. Leonardo, S., Alberto, A., Raul, R., Antonio, A., and Cristobal, N. 2011. Ellagic acid: biological properties and biotechnological development for production processes. African Journal of Biotechnology 10 (45): 18-23.
17. Liu, Y., Liu, Y., Liu, H., and Shang, Y. 2018. Evaluating effects of ellagic acid on the quality of kumquat fruits during storage. Scientia Horticulturae 227: 244-254. https://doi.org/10.1016/j.scienta.2017.08.055
18. Lopez, S. G., and Luderer, U. 2004. Effects of cyclophosphamide and buthionine sulfoximine on ovarian glutathione and apoptosis. Free Radical Biology and Medicine 36 (11): 1366-1377. https://doi: 10.1016/j.freeradbiomed.2004.02.067.
19. Mangena, P. 2021. Analysis of correlation between seed vigour, germination and multiple shootinduction in soybean (Glycine max Merr.). Heliyon 7 (1): 1-8. https://doi: 10.1016/j.heliyon.2021.e07913
20. Marthandan, V., Geetha, R., Kumutha, K., Renganathan, V. G., Karthikeyan, A., and Ramalingam, J. 2020. Seed priming: A feasible strategy to enhance drought tolerance in crop plants. International Journal of Molecular Sciences 21: 58-82. https://doi: 10.3390/ijms21218258
21. Motacefi, M., Sirousmehr, A., and Mohsen Mousavi Nik, M. 2021. Effect of organic growth stimulator on yield, yield components, oil percentage, and‎ some physiological indices of safflower under drought stress condition. Journal of Crops Improvement 23 (3): 335-348. https://doi.10.22059/JCI.2021.300601.2378
22. Movahhedi Dehnavi, M., Niknam, N., Behzadi, Y., and Mohtashami, R. 2017. Comparison of physiological responses of linseed (Linum usitatissimum) to drought and salt stress and salicylic acid foliar application. Iranian Journal of Plant Biology 9 (3): 39-62. (in Persian with English abstract).
23. Nazari, R., Parsa, S., Tavakol Afshari, R., and Mahmoodi, S. 2020. Salicylic acid priming before and after accelerated aging process increases seedling vigor in aged soybean seed. Journal of Crop Improvement 34 (2): 1-20. (in Persian with English abstract). https://doi.org/10.1080/15427528.2019.1710734
24. Oliveira, K. R., Sampaio, F. R., Siqueira, G. S., Galvao, I. M., Bennett, S. J., Gratao, P. L., and Barbosa, R. M. 2021. Physiological quality of soybean seeds grown under different low altitude field environments and storage time. Plant, Soil and Environment 67 (2): 92-98. https://doi.org/10.17221/512/2020-PSE
25. Onder, S., Tonguc, M., Guvercin, D., and Karakurt, Y. 2020. Biochemical changes stimulated by accelerated aging in safflower seeds (Carthamus tinctorius) Journal of Seed Science 42: 1-12. https://doi.org/10.1590/2317-1545v42227873
26. Oomah, B. D., Mazza, G., and Przyblski, R. 1995. Comparison of flaxseed meal lipids extracted with different solvent. Journal of Food Science and Technology 29: 654-658. https://doi.12691/jfnr-2-1-10
27. Pari, L., and Sivasankari, R. 2008. Effect of ellagic acid on cyclosporine A-induced oxidative damage in the liver of rats. Fundamental & Clinical Pharmacology 22: 395-401. https://doi.org/10.1111/j.1472-8206.2008.00609.x
28. Rahimi, G., Bradaran Firouzabadi, M., Makarian, H., and Gholipoor, M. 2014. The effect of seed aging and pretreatment with pyridoxine on growth and yield of soybean in weed competition. M.Sc. Thesis. Shahrood University of Technology. Iran. (in Persian with English abstract).
29. Sadeghi, H., Shaeidaei, A., Gholami, H., and Yari, L. 2014. Effect of packaging materials, storage duration and conditions on seed germination traits in laboratory and field emergence of soybean seedling. Iranian Journal of Seed Science and Research 1(1): 67-82.
30. Saha, R. R., and Sultana, W. 2008. Influence of seed ageing on growth and yield of soybean. Bangladesh Journal of Botany 37 (1): 21-26.
31. Santos, R. F., Placido, H. F., Bosche, L. L., Neto, H. Z., Ferando, H., and Alessandro, B. 2021. Accelerated aging methodologies for evaluating physiological potential of treated soybean seeds. Journal of Seed Science 43 (4): 1-10. https://doi.org/10.1590/2317-1545v43250605
32. Sheidaei, S., Hamidi, A., Sadeghi, H., Oskouei, B., and Zare, L. 2019. Impact of storage fungi on soybean seed deterioration in different storage conditions and seed moisture content. Iranian Journal of Seed Science and Technology 6 (1): 65-76. (in Persian with English abstract).
33. Taji, M., Rahemi karizaki, A., and Daneshmand khosravi, K. 2014. Effect of seed aging on emergence and morphological characteristics of sunflower cultivars (Helianthus annus). Journal of Applied Research of Plant Ecophysiology 1 (4): 19-30. (in Persian with English abstract).
34. Turk, G., Ceribas, A. O., Sahna, E., and Atessahin, A. 2011. Lycopene and ellagic acid prevent testicular apoptosis induced by cisplatin. Phytomedicine 18 (5): 356-361. DOI: 1016/j.phymed.2010.07.008
35. Verma, S. K., Bjpai, G. C., Tewari, S. K., and Singh, J. 2005. Seedling index and yield as influenced by seed size in pigeon pea. Legume Resarch 28: 143-145.
36. Weerasekara, I., Sinniah, U. R., Namasivayam, P., Nazli, M. H., Abdurahman, S. A., and Ghazali, M. N. 2021. Priming with humic acid to reverse ageing damage in soybean (Glycine max (L.) Merrill.] seeds. Agriculture 11 (966): 1-18. https://doi.org/10.3390/agriculture11100966
37. Xiong, Z. T., Chao, L., and Bing, G. 2006. Phytotoxic effects of copper on nitrogen metabolism and plant growth in Brassica pekinensis Ecotoxicology and Environmental Safety 64: 273-280. https://doi.org/10.1016/j.ecoenv.2006.02.003
38. Yang, J., Zhang, J., Huang, Z., Zhu, Q., and Wang, L. 2000. Remobilization of carbon reserved is improved by controlled soil drying during grain filling of wheat. Crop Science 40: 1645-1655. https://doi.org/10.2135/cropsci2000.4061645x